[1]张正顺,张艳霞△,陈绍仪,等.当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的影响[J].西部中医药,2018,31(09):8-12.
　ZHANG Zhengshun,ZHANG Yanxia,CHEN Shaoyi,et al.Effects of Volatile Oil from DangGui on Cell Cycle and Proliferation of Human Lung Adenocarcinoma GLC-82 Cells[J].Western Journal of Traditional Chinese Medicine,2018,31(09):8-12.
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当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年09期
页码:
8-12
栏目:
出版日期:
2018-09-15

文章信息/Info

Title:
Effects of Volatile Oil from DangGui on Cell Cycle and Proliferation of Human Lung Adenocarcinoma GLC-82 Cells
文章编号:
1004-6852(2018)09-0008-05
作者:
张正顺1，张艳霞2△，陈绍仪3，孔学礼2，周佳玮2
1 甘肃省中医院白银分院，甘肃 白银 730900； 2 甘肃中医药大学/甘肃省中药药理与毒理学重点实验室/甘肃省高校中(藏)药化学与质量研究省级重点实验室； 3 白银市第二人民医院
Author(s):
ZHANG Zhengshun1, ZHANG Yanxia2△, CHEN Shaoyi 3, KONG Xueli 2, ZHOU Jiawei 2
1 Baiyin Branch, Gansu Provincial Hospital of Traditional Chinese Medicine, Baiyin 730900, China; 2 Gansu University of Chinese Medicine/Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicines of Gansu Province(nurturing station)/ Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Gansu University of Traditional Chinese Medicine; 3 The Second People′s Hospital of Baiyin City
关键词:
人肺腺癌GLC-82细胞; 细胞周期; 当归挥发油
Keywords:
human lung adenocarcinoma GLC-82 cells;  cell cycle;  volatile oil from DangGui
分类号:
R734
文献标志码:
A
摘要:
目的：探讨当归挥发油对人肺腺癌GLC-82细胞增殖及细胞周期的作用。方法：采用噻唑蓝(MTT)比色法检测当归挥发油对GLC-82细胞存活率的影响；倒置显微镜下观察细胞生长形态；流式细胞仪检测当归挥发油对GLC-82细胞周期的影响。结果：在6.25～200 μg/mL浓度范围内当归挥发油对GLC-82细胞增殖具有浓度依赖性抑制作用，当归挥发油对GLC-82细胞的IC50为113.03 μg/mL；倒置显微镜下观察发现阴性组细胞具有GLC-82细胞典型的形态特征，多边形，成片生长，随着当归挥发油浓度的升高，细胞数量减少，散在生长，形态发生变化，逐渐皱缩；经当归挥发油处理后GLC-82细胞表现出明显的G2期周期阻滞。结论：当归挥发油可抑制GLC-82细胞生长，引起细胞周期G2期的阻滞。
Abstract:
Objective: To discuss the effects of volatile oil from DangGui(Angelica sinensis) on cell cycle and the proliferation of human lung adenocarcinoma GLC-82 cells. Methods: The effects of volatile oil from DangGui on survival rate of GLC-82 cells were detected by using MTT chromometry; cell growth morphology was observed under the inverted microscope; the influence of GLC-82 cell cycle was measures by flow cytometry. Results: Volatile oil from DangGui inhibited the proliferation of GLC-82 cells in concentration-dependent manner ranged between 6.25~200 μg/mL, IC50 of volatile oil from DangGui to GLC-82 cells was 113.03 μg/mL; the results under the inverted microscope showed that the cells in negative group presented typical morphological features of GLC-82 cells, polygon, flaky growth, as the concentrations of volatile oil from DangGui raised, cellular numbers reduced, spread in growth, the shape has changed and shrunk gradually; the results of flow cytometry indicated that GLC-82 cells performed the obvious cell cycle arrest at phase G2 after dealing with volatile oil from DangGui. Conclusion: Volatile oil from DangGui could inhibit the growth of GLC-82 cells and cause cell cycle arrest at phase G2.

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备注/Memo

备注/Memo:
收稿日期：2017-10-28 *基金项目：白银市科技计划项目(编号2013-2-76Y)；甘肃中医学院中青年科研基金项目(编号2305016601)。 作者简介：张正顺(1973—)，男，主任医师。研究方向：超声医学。 △通讯作者：张艳霞(1981—)，女，硕士学位，副教授。研究方向：肿瘤药理学。
更新日期/Last Update: 2018-09-15