[1]吴敏,李丽,顾文燕.β-榄香烯对结肠癌细胞耐长春新碱的影响[J].西部中医药,2020,33(07):28-32.[doi:10.12174/j.issn.1004-6852.2020.07.07]
 WU Min,LI Li,GU Wenyan.Effects of β-elemene on Vincristine of Colon Cancer Cells[J].Western Journal of Traditional Chinese Medicine,2020,33(07):28-32.[doi:10.12174/j.issn.1004-6852.2020.07.07]
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β-榄香烯对结肠癌细胞耐长春新碱的影响
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
33
期数:
2020年07期
页码:
28-32
栏目:
方药·药理研究
出版日期:
2020-07-15

文章信息/Info

Title:
Effects of β-elemene on Vincristine of Colon Cancer Cells
文章编号:
1004-6852(2020)07-0028-05
作者:
吴敏李丽顾文燕
恩施土家族苗族自治州中心医院手术室,湖北 恩施 445000
Author(s):
WU Min LI Li GU Wenyan△
Operation Room, The Central Hospital of Enshi Tujia and Miao Autonomous Perfecture, Enshi 445000, China
关键词:
结肠癌细胞β-榄香烯长春新碱耐药
Keywords:
colon cancer cells β-elemene vincristine drug-resistance
分类号:
R285.5
DOI:
10.12174/j.issn.1004-6852.2020.07.07
文献标志码:
A
摘要:
目的:观察β-榄香烯对结肠癌细胞(SW-480)耐长春新碱(vincristine,VCR)的影响。方法:体外传代培养SW-480细胞,采用CCK8法检测β-榄香烯和VCR对SW-480细胞增殖活力的影响;流式细胞仪检测β-榄香烯和VCR对SW-480细胞凋亡的影响;Western Blot方法分析β-榄香烯对细胞中WEE1蛋白表达的影响,并采用pcDFNA-WEE1质粒过表达技术对SW-480细胞中WEE1蛋白进行过表达。结果:β-榄香烯可以诱导VCR对结肠癌SW-480细胞增殖活力的抑制作用;β-榄香烯+VCR联合组SW-480细胞凋亡率为(35.12±6.39)%,高于VCR干预组的(22.66±5.37)%(P<0.05);β-榄香烯呈剂量、时间依赖性地抑制SW-480细胞中WEE1激酶的表达(P<0.05);pcDFNA-WEE1质粒转染能明显上调SW-480细胞中WEE1激酶表达,并且降低了β-榄香烯+VCR对结肠癌SW-480细胞增殖活力的抑制作用(P<0.05)。结论:β-榄香烯通过抑制WEE1表达,增强VCR对结肠癌细胞化疗敏感性的能力,并促使结肠癌细胞凋亡。
Abstract:
Objective: To observe the effects of β-elemene on vincristine (VCR) of colon cancer cells (SW-480). Methods: SW-480 cells were subcultured in vitro, CCK8 method was used to detect the effects of β-elemene and VCR on proliferation activity of SW-480 cells; flow cytometry to survey their influence on the apoptosis of SW-480 cells; Western blot method to analyze the effects of β-elemene on the expressions of cellular WEE1 protein, pcDFNA-WEE1 plasmid overexpression technique to perform the overexpression of WEE1 protein in SW-480 cells. Results: β-elemene could induce the inhibitory effects of VCR on the proliferation activity of colon cancer cells SW-480 cells; SW-480 cellular apoptosis rate of the combination group of β-elemene+VCR was ((35.12±6.39)%, higher than (22.66±5.37)% of VCR intervention group (P<0.05); β-elemene inhibited the expressions of WEE1 kinase in SW-480 cells in the dose and time dependent manner (P<0.05); pcDFNA-WEE1 plasmid transfection could obviously raise the expressions of WEE1 kinase in SW-480 cells, and relieve the inhibitory effects of β-elemene+VCR on proliferation activty of SW-480 cells in colon cancer cells (P<0.05). Conclusion: β-elemene could enhance the tolerance of VCR to chemotherapy of colon cancer cells through inhibiting the expressions of WEE1, and promote the apoptosis of colon cancer cells.

相似文献/References:

[1]熊刚,邹华,胡承莲,等.绿原酸基于PI3K/Akt信号通路对结肠癌细胞增殖和凋亡的调控作用[J].西部中医药,2020,33(05):71.[doi:10.12174/j.issn.1004-6852.2020.05.20]
 XIONG Gang,ZOU Hua,HU Chenglian,et al.Effects of Chlorogenic Acid on the Proliferation and Apoptosis of Colon Cancer Cells Mediated by P13k/Akt Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2020,33(07):71.[doi:10.12174/j.issn.1004-6852.2020.05.20]

备注/Memo

备注/Memo:
收稿日期:2019-02-12作者简介:吴敏(1982—),女,主治医师。研究方向:消化道恶性肿瘤耐药研究。△通讯作者:顾文燕(1986—),女,主治医师。研究方向:消化道恶性肿瘤耐药研究。
更新日期/Last Update: 2020-07-15