[1]李欢,卢玲,廖现秋,等.基于网络药理学探讨天南星治疗癫痫的作用机制[J].西部中医药,2022,35(10):23-28.[doi:10.12174/j.issn.2096-9600.2022.10.06]
　LI Huan,LU Ling,LIAO Xianqiu,et al.The Effects of Tiannanxing in the Treatment of Epilepsy Based on Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2022,35(10):23-28.[doi:10.12174/j.issn.2096-9600.2022.10.06]
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基于网络药理学探讨天南星治疗癫痫的作用机制

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年10期
页码:
23-28
栏目:
出版日期:
2022-10-15

文章信息/Info

Title:
The Effects of Tiannanxing in the Treatment of Epilepsy Based on Network Pharmacology
作者:
李欢1, 卢玲1, 廖现秋1, 何乾超2, 陈炜2, 梁霜2, 刁丽梅2
1.广西中医药大学研究生学院,广西 南宁 530001
2.广西中医药大学第一附属医院
Author(s):
LI Huan1, LU Ling1, LIAO Xianqiu1, HE Qianchao2, CHEN Wei2, LIANG Shuang2, DIAO Limei2
1.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China
2.The First Affiliated Hospital of Guangxi University of Chinese Medicine
关键词:
癫痫; 网络药理学; 天南星; 有效成分; 机制
Keywords:
epilepsy; network pharmacology; active ingredients; mechanism
分类号:
R742.1
DOI:
10.12174/j.issn.2096-9600.2022.10.06
文献标志码:
A
摘要:
目的基于网络药理学探寻天南星治疗癫痫的潜在有效成分及作用机制。 方法根据口服生物利用度（oral bioavailability， OB）≥30%、类药性（drug likeness，DL）从中药系统药理学技术平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）中筛选出天南星的活性成分、作用靶点。通过 GeneCards、OMIM数据库寻找癫痫的相应靶点，将天南星作用靶点与癫痫相关靶点取交集，筛选出天南星治疗癫痫的活性成分及作用靶点。运用Cytoscape 3.7.2软件构建天南星治疗癫痫的“药物-活性成分-疾病靶点”网络。通过STRING数据库构建靶标蛋白相关作用网络并进行关键靶标的生物功能注释及通路分析。 结果1）天南星中有效活性成分有6种，作用于26个癫痫靶点；关键活性成分包括8、11、14-二十二碳三烯酸甲酯、24-表蛇甾醇、β-谷甾醇、谷甾醇、豆甾醇等。2）网络分析显示天南星治疗癫痫的核心靶点包括CASP3、ADRA1B、JUN、MAOA、CASP8、CASP9、MAOB、PTGS2、SLC6A4、ADRB2、BAX、BCL2、PRKCA等。3）基因富集分析得到GO功能条目84个，主要包括类固醇激素受体活性、核受体活性、转录因子活性、细胞因子活性、细胞因子受体结合、核糖核酸聚合酶二转录因子结合、泛素样蛋白连接酶结合等；KEGG富集通路74条，主要涉及富集在细胞凋亡-多种信号通路、乙型肝炎信号通路、人类免疫缺陷病毒1型感染信号通路、p53信号通路、铂耐药信号通路、细胞凋亡信号通路、EB病毒感染信号通路、肌萎缩性脊髓侧索硬化症（amyotrophic lateral sclerosis，ALS）信号通路、人类巨细胞病毒感染信号通路等通路。 结论基于网络药理学探讨天南星治疗癫痫的作用靶点及信号通路，为进一步研究其治疗癫痫的作用机制提供了新的线索。
Abstract:
ObjectiveTo explore the potential active ingredients and the mechanism of Tiannanxing (Arisaemetis rhizoma) in treating epilepsy based on network pharmacology. MethodsAccording to OB≥30% and DL, the active ingredients and the targets of Tiannanxing were screened from TCMSP. The corresponding targets of epilepsy were surveyed from GeneCards and OMIM databases, the active ingredients and the targets of Tiannanxing in treating epilepsy were investigated when the targets of Tiannanxing and the related targets of epilepsy intersected. The network of "disease-active ingredients-disease targets" of the treatment of epilepsy by Tiannanxing was constructed by using Cytoscape 3.7.2 software. Target protein associated action network was built via STRING databases, and the annotation of biological function and pathway analysis of key targets were carried out. Results1)There were six active ingredients contained in Tiannanxing, acting on 26 epilepsy targets; the principal active ingredients included 8, 11, 14-docosatrienoate methyl ester, 24-epistosterol, β-sitosterol, sitosterol and stigmasterol. 2) Network analysis displayed that the core targets of the treatment contained CASP3, ADRA1B, JUN, MAOA, CASP8, CASP9, MAOB, PTGS2, SLC6A4, ADRB2, BAX, BCL2, PRKCA and others. 3) Gene enrichment analysis indicated that there were 84 GO function items, mainly containing steroid hormone receptor activity, nuclear receptor activity, transcription factors activity, cell factor activity, cytokine receptor binding, and ribonucleic acid polymerase 2 transcription factor binding, as well as ubiquitin-like protein ligase binding; 74 KEGG enrichment pathways, mainly involved in enrichment in apoptosis - multiple signaling pathways, hepatitis B signaling pathway, human immunodeficiency virus type 1 infection signaling pathway, p53 signaling pathway, platinum resistance signaling pathway, apoptosis signaling pathway, EB virus infection signaling pathway, ALS signaling pathway and human cytomegalovirus infection signaling pathway. ConclusionThe paper discusses the targets and signaling pathway of the treatment based on network pharmacology, which could provide new clue for further study on the mechanism of treating epilepsy.

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备注/Memo

备注/Memo:
李欢（1994—），女，在读硕士研究生。研究方向：癫痫的基础与临床研究。国家自然科学基金地区基金（81760809）；广西壮族自治区科学技术厅面上项目（2017GXNSFAA198294）；广西卫生适宜技术研究与开发项目（S2017049）；广西中医药大学岐黄工程高层次人才团队培育项目（030030001-04B1804803-500101）；广西中医药大学第一附属医院学术团队（院字2018（146）号）；广西研究生教育创新计划项目（YJSY20190062）。
更新日期/Last Update: 2022-10-15