[1]赵耀,王立恒,李凤岩,等.针刀联合葛根素膝关节内注射治疗兔膝骨关节炎的实验研究[J].西部中医药,2023,36(10):27-30.[doi:10.12174/j.issn.2096-9600.2023.10.05]
　ZHAO Yao,WANG Liheng,LI Fengyan,et al.Experimental Study on Needle-knife Combined with Intra-articular Injection of Puerarin Injection in the Treatment of Rabbit with Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2023,36(10):27-30.[doi:10.12174/j.issn.2096-9600.2023.10.05]
点击复制

针刀联合葛根素膝关节内注射治疗兔膝骨关节炎的实验研究
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年10期
页码:
27-30
栏目:
出版日期:
2023-10-15

文章信息/Info

Title:
Experimental Study on Needle-knife Combined with Intra-articular Injection of Puerarin Injection in the Treatment of Rabbit with Knee Osteoarthritis
作者:
赵耀1, 王立恒1, 李凤岩2, 董嵩2, 苗兰英3, 林大勇3, 张丽萍3
1.大连市第二人民医院，辽宁 大连 116011
2.朝阳市第二医院，辽宁 朝阳 122000
3.辽宁中医药大学，辽宁 沈阳 110032
Author(s):
ZHAO Yao1, WANG Liheng1, LI Fengyan2, DONG Song2, MIAO Lanying3, LIN Dayong3, ZHANG Liping3
1.The Second People's Hospital of Dalian, Dalian 116011, China
2.The Second Hospital of Chaoyang, Chaoyang 122000, China
3.Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China
关键词:
骨关节炎; 针刀; 葛根素; 白细胞介素1; 白细胞介素6; 肿瘤坏死因子α; 基质金属酶蛋白; 兔; 动物实验
Keywords:
osteoarthritis; needle-knife; puerarin; IL-1; IL-6; TNF-α; MMP; rabbit; zoopery
分类号:
R684.3
DOI:
10.12174/j.issn.2096-9600.2023.10.05
文献标志码:
A
摘要:
目的探讨针刀联合葛根素膝关节腔内注射对兔膝骨关节炎模型的影响。 方法选择新西兰白兔30只，随机分为空白对照组、模型组、葛根素组、针刀联合葛根素组和针刀联合透明质酸钠组，每组6只。除空白对照组外，其余4组均制备兔膝骨关节炎动物模型。造模成功后，葛根素组关节腔内注入葛根素注射液，针刀联合葛根素组针刀松解术的同时注入葛根素注射液，针刀联合透明质酸钠组予以针刀松解术的同时注入透明质酸钠，空白对照组和模型组注射等体积的生理盐水，各组均连续治疗5周。RT-PCR检测膝兔软骨检测基质金属酶蛋白1（matrix metalloproteinases-1，MMP-1）和基质金属酶蛋白13（matrix metalloproteinases-1，MMP-13）的mRNA含量，ELISA法检测兔关节腔内细胞因子肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）和白细胞介素1（interleukin-1，IL-1）、白细胞介素6（Interleukin-1，IL-6）的含量。 结果针刀联合葛根素组中IL-1、IL-6和TNF-α水平及MMP-1和MMP-13的mRNA表达较其他组明显减少（P＜0.05）。 结论针刀联合葛根素可以明显减少软骨关节肿MMP-1和MMP-13的mRNA含量，降低IL-1、IL-6和TNF-α细胞因子表达。
Abstract:
ObjectiveTo explore the influence of needle-knife and intra-articular injection of puerarin injection on rabbit models suffering knee osteoarthritis (KOA). MethodsAll 30 New Zealand rabbits were randomized into blank control group, the model group, puerarin group, needle-knife combined with puerarin group, needle-knife and sodium hyaluronate group, with six ones in each group. Except for the blank control group, the remaining four groups were prepared into KOA rabbit models. After successful modeling, puerarin group accepted intra-articular injection of puerarin injection, needle-knife combined with puerarin group needle-knife relaxation and puerarin injection, blank control group and the model group were injected with equal volume of physiological saline, consecutively treated for five weeks. RT-PCR was used to detect the contents of MMP-1 and MMP-13 mRNA in rabbit knee cartilage, ELISA method to detect the expressions of TNF-α, IL-1 and IL-6 in knee articular cavity. ResultsThe expressions of IL-1, IL-6 and TNF-α, MMP-1 and MMP-13 mRNA in needle-knife combined with puerarin group reduced remarkably than the other groups (P<0.05). ConclusionNeedle-knife and intra-articular injection of puerarin injection could apparently lower the contents of MMP-1 and MMP-13 mRNA in knee cartilage and the expressions of IL-1, IL-6 and TNF-α.

相似文献/References:

[1]宋渊,李亚军,梁雄勇,等.针刀治疗神经根型颈椎病研究现状[J].西部中医药,2011,24(11):40.
[2]黄 菁,乌英别兢,建 军.针刀配合低浓度药物治疗跟痛症43例疗效观察[J].西部中医药,2012,25(10):9.
　HUANG Jing,WUYING Bie-jing,JIAN Jun. Curative Effects of Needle-knife Combined with Low Concentration Medicine in Treating 43 Cases of Calcanodynia[J].Western Journal of Traditional Chinese Medicine,2012,25(10):9.
[3]张建林.中药熏洗联合关节内注射玻璃酸钠治疗膝骨关节炎30例疗效观察[J].西部中医药,2012,25(09):80.
　ZHANG Jian-lin.Curative Observation on Fumigation with Herb and Intra-Articular Sodium Hyaluronate Injection in Treating 30 Cases of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2012,25(10):80.
[4]魏千程,燕忠生,丁辉,等.“Z”型拨针刀治疗颈项部肌筋膜炎45例[J].西部中医药,2014,27(02):120.
　WEI Qiancheng,YAN Zhongsheng,DING Hui,et al.Treatment for 45 Cases of Cervical Myofascitis by Z-shaped Needle-knife[J].Western Journal of Traditional Chinese Medicine,2014,27(10):120.
[5]周立亚,刘德玉,李福林,等.热敷散外敷治疗兔骨性关节炎的机理研究[J].西部中医药,2014,27(10):22.
[6]吕凤立,高莹,杨建.腰三横突综合征中医治疗研究进展[J].西部中医药,2015,28(01):142.
[7]鄢卫平,王玉泉.针刀松解枕下三角配合旋牵手法治疗椎动脉型颈椎病75例临床观察[J].西部中医药,2012,25(07):83.
　YAN Wei-ping,WANG Yu-quan.Clinical Observation on Needle-knife in Releasing Suboccipital Triangle and Rotation-Traction Manipulation in Treating 75 Cases of Cervical Spondylosis of Vertebral Artery Type[J].Western Journal of Traditional Chinese Medicine,2012,25(10):83.
[8]王亚伟,张彦军.中西医综合治疗膝骨性关节炎疗效观察[J].西部中医药,2012,25(05):80.
　WANG Ya-wei,ZHANG Yan-jun.Observation on Comprehensive Treatment of Knee Osteoarthritis with Integrated Traditional Chinese and Western Medicine[J].Western Journal of Traditional Chinese Medicine,2012,25(10):80.
[9]鄢卫平,李景周,强天明.针刀治疗强直性脊椎炎60例[J].西部中医药,2013,26(12):118.
　YAN Weiping,LI Jingzhou,QIANG Tianming.Treating 60 Cases of Ankylosing Spondylitis by Needle-knife[J].Western Journal of Traditional Chinese Medicine,2013,26(10):118.
[10]薄云,夏冰杰,赵丽艳,等.疏肝滋肾汤治疗绝经后女性骨关节炎的临床观察[J].西部中医药,2016,29(07):93.
　BO Yun,XIA Bingjie,ZHAO Liyan,et al.Clinical Observation on ShuGan ZiShen Tang in Osteoarthritis of the Female Patients after the Menopause[J].Western Journal of Traditional Chinese Medicine,2016,29(10):93.
[11]李粟玉,陈广峰,郝锦秋,等.针刀治疗膝骨关节炎的施术部位和机制研究[J].西部中医药,2022,35(12):163.[doi:10.12174/j.issn.2096-9600.2022.12.38]
　LI Suyu,CHEN Guangfeng,HAO Jinqiu,et al.Study on the Operation Sites of Treating Knee Osteoarthritis by Acupotomy and the Mechanism[J].Western Journal of Traditional Chinese Medicine,2022,35(10):163.[doi:10.12174/j.issn.2096-9600.2022.12.38]

备注/Memo

备注/Memo:
赵耀（1979—），男，硕士学位，主任医师。研究方向：骨科康复，运动损伤及颈肩腰腿痛的针灸推拿治疗。辽宁省自然科学基金（20180550084）；国家中医药管理局全国名老中医药专家传承工作室建设项目（周升平全国名老中医药专家传承工作室）。
更新日期/Last Update: 1900-01-01