[1]王欣,吴银瓶,鞠洋,等.红毛五加叶水提取物对大鼠局灶性脑缺血损伤的预防作用[J].西部中医药,2024,37(05):44-47.[doi:10.12174/j.issn.2096-9600.2024.05.06]
　WANG Xin,WU Yinping,JU Yang,et al.Preventive Effect of Aqueous Extract of Hongmao Wujia Leaves on Focal Cerebral Ischemic Injury in Rats[J].Western Journal of Traditional Chinese Medicine,2024,37(05):44-47.[doi:10.12174/j.issn.2096-9600.2024.05.06]
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红毛五加叶水提取物对大鼠局灶性脑缺血损伤的预防作用
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年05期
页码:
44-47
栏目:
基础研究
出版日期:
2024-05-15

文章信息/Info

Title:
Preventive Effect of Aqueous Extract of Hongmao Wujia Leaves on Focal Cerebral Ischemic Injury in Rats
作者:
王欣, 吴银瓶, 鞠洋, 孟璇
兰州大学第二医院，甘肃 兰州 730030
Author(s):
WANG Xin, WU Yinping, JU Yang, MENG Xuan
Lanzhou University Second Hospital, Lanzhou 730030, China
关键词:
局灶性脑缺血; 一氧化氮合酶; 一氧化氮; 红毛五加叶水提取物; 大鼠
Keywords:
focal cerebral ischemia; nitric oxide synthase; nitric oxide; aqueous extract of leaf; rats
分类号:
R235.5
DOI:
10.12174/j.issn.2096-9600.2024.05.06
文献标志码:
A
摘要:
目的研究红毛五加叶水提取物（acanthopanax giraldii leaves extract，AGLE）对大鼠局灶性脑缺血损伤的预防作用。 方法将138只SPF级雄性SD大鼠随机分为假手术组、模型组、银杏叶片组（0.04 g/kg）及AGLE高、中、低剂量组（20、10、5 g/kg），每组23只，采用插线法阻塞大鼠大脑中动脉（middle cerebral artery，MCA）制备局灶性脑缺血模型；采用氯化三苯基四氮唑（2.3.5-Triphenytetrya-zolium chloride，TTC）染色法测定脑梗死范围；Longa法观测大鼠神经功能缺失评分；光镜下观察缺血侧大脑皮层病理学改变；分光光度法和硝酸还原酶法分别测定脑组织一氧化氮合酶（nitric oxide synthase，NOS）活性及一氧化氮（nitric oxide，NO）含量。 结果模型组大鼠行为指标评分、脑梗死范围、NOS活性及NO含量均高于假手术组（P＜0.01）；缺血侧大脑皮层出现神经元结构不清，神经细胞肿胀，核固缩、溶解、破裂或消失等病理改变。与模型组比较，AGLE各剂量组大鼠行为指标评分、脑梗死范围、NOS活性及NO含量均降低（P＜0.01）。与银杏叶片组比较，AGLE低剂量组改善大鼠行为指标评分更明显（P＜0.01）。AGLE各剂量组脑梗死范围、NOS活性及NO含量与银杏叶片组比较，差异无统计学意义（P＞0.05）。 结论AGLE对大鼠局灶性脑缺血损伤具有预防作用，其作用机制可能与降低脑组织中NOS活性及NO含量有关。
Abstract:
ObjectiveTo study the protective effect of aqueous extract of Hongmao Wujia leaves (acanthopanax giraldii leaves extract, AGLE) on focal cerebral ischemic injury in rats. MethodsAll 138 male SD rats were randomly divided into sham operation group, the model group, ginkgo biloba group (0.04 g/kg), and high, medium, and low dose groups (20, 10, and 5 g/kg) of AGLE, 23 ones in each group and focal cerebral ischemia model was prepared using the middle cerebral artery occlusion (MCAO) of rats by the wire-insertion method; the extent of cerebral infarction was determined by TTC staining; the neurological deficit scores of the rats were observed by the Longa method; and the pathological changes in the cerebral cortex on the ischemic side were observed under light microscope; the nitric oxide synthase (NOS) activity and nitric oxide (NO) content of brain tissue were measured by spectrophotometry and nitrate reductase method, respectively. ResultsBehavioral index scores, extent of cerebral infarction, NOS activity and NO content were higher in the model group than in the sham-operated group (P<0.01); the cerebral cortex on the ischemic side showed pathological changes such as neuronal structural ambiguity, neuronal cell swelling, and nuclear consolidation, lysis, rupture or disappearance. Behavioral index scores, extent of cerebral infarction, NOS activity and NO content were all reduced in each dose group of AGLE compared with the model group (P<0.01). Compared with ginkgo biloba group, behavioral index scores were improved more obviously in low dose group of AGLE (P<0.01). The difference had no statistical meaning in the comparisons of extent of cerebral infarction, NOS activity and NO content between different doses groups of AGLE (P>0.05). ConclusionAGLE has a preventive effect on focal cerebral ischemic injury in rats, and its mechanism of action may be related to the reduction of NOS activity and NO content in brain tissue.

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备注/Memo

备注/Memo:
王欣（1973—），男，主任医师。研究方向：血管超声诊断。E-mail：372802125@qq.com。甘肃省科技计划项目（20JR5RA326）。
更新日期/Last Update: 2024-05-15