[1]王林凤,魏国玲,康毅.蒲地兰颗粒的质量控制[J].西部中医药,2014,27(04):17-20.
　WANG Linfeng,WEI Guoling,KANG Yi.Quality Control of PuDiLan Granules[J].Western Journal of Traditional Chinese Medicine,2014,27(04):17-20.
点击复制

蒲地兰颗粒的质量控制
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年04期
页码:
17-20
栏目:
出版日期:
2014-04-15

文章信息/Info

Title:
Quality Control of PuDiLan Granules
文章编号:
1004-6852(2014)04-0017-04
作者:
王林凤，魏国玲，康毅
陕西中医学院附属医院，陕西 咸阳 712000
Author(s):
WANG Linfeng, WEI Guoling, KANG Yi
Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
关键词:
蒲地兰颗粒; 质量控制; 薄层色谱; 高效液相色谱法; 黄芩苷
Keywords:
PuDiLan granules;  quality control;  thin-layer chromatography;  high liquid chromatography; baicalin
分类号:
R284
文献标志码:
A
摘要:
目的：建立蒲地兰颗粒的质量控制方法。方法：采用薄层色谱法对黄芩进行定性鉴别，采用HPLC法对黄芩中的黄芩苷进行含量测定。色谱柱为Thermo C18(250 mm×4.6 mm，5 μm)，流动相为甲醇-0.2%磷酸水溶液(47∶53)，流速为1.0 mL/min，检测波长为280 nm。柱温为室温。结果：薄层色谱斑点清晰，阴性对照无干扰。黄芩苷在0.076～7.6 μg范围内线性关系良好(r=0.9999)，平均回收率为99.5%，RSD=0.71%(n=6)。结论：本研究建立的TLC和HPLC方法操作简便，结果准确，重复性好，专属性强，可用于该制剂的质量控制。
Abstract:
Objective: To establish the method for quality control of PuDiLan granules. Methods: HuangQin (Scutellaria baicalensis) was identified qualitatively by TCL, the contents of baicalin in HuangQin were determined by HPLC. Chromatographic column: Thermo C18 (250 mm×4.6 mm, 5 μm), mobile phase: methanol-0.2% phosphoric acid solution (47:53), flow rate was 1.0mL/min, detection wavelength: 280nm, room temperature. Results: The spots of TLC were distinguished and clear. Baicalin showed better linear relationship in the range between 0.076 and 7.6 μg (r=0.9999), average recovery was 99.5%, RSD=0.71%(n=6). Conclusion: TLC and HPLC method established in the study, simple, accurate, reproducible and specific, could be used to quality control of the preparation.

相似文献/References:

[1]陈涛.层级全责护理模式在护理质量控制中的应用[J].西部中医药,2013,26(12):124.
　CHEN Tao.Application of Hierarchical Full-responsibility Nursing System to Quality Control of Care[J].Western Journal of Traditional Chinese Medicine,2013,26(04):124.
[2]刘光斌,毛和平,谢六生,等.金锁二仙口服液质量标准研究[J].西部中医药,2014,27(12):21.
[3]张伟,王信,李彩东△,等.蛭龙镇咳通瘀胶囊中次黄嘌呤含量测定及峰归属研究[J].西部中医药,2015,28(12):38.
[4]殷建峰.朴硝散的制备及质量控制[J].西部中医药,2015,28(03):34.
[5]李妍芃,刘曦,臧艺玫,等.桑白皮的颜色与1-脱氧野尻霉素含量的相关性研究[J].西部中医药,2016,29(07):26.
　LI Yanpeng,LIU Xi,ZANG Yimei,et al.Study on the Relativity Between the Color of SangBaiPi and the Contents of 1-Deoxynojirimycin[J].Western Journal of Traditional Chinese Medicine,2016,29(04):26.
[6]文艳梅.互联网平台管理模式对消毒供应中心持续性质量改进的实效[J].西部中医药,2018,31(07):58.
　WEN Yanmei.Effects of Internet Platform Management Model on Continuous Quality Improvement of Central Sterile Supply Department[J].Western Journal of Traditional Chinese Medicine,2018,31(04):58.
[7]焦正花,李文萍,赵凤菊,等.清热解毒冲洗液质量标准研究[J].西部中医药,2018,31(08):16.
　JIAO Zhenghua,LI Wenping,ZHAO Fengju,et al.Study on Quality Standard of Heat-clearing Detoxifying Flush Fluid[J].Western Journal of Traditional Chinese Medicine,2018,31(04):16.
[8]张平,李冬华,王晓琳,等.不同产地及加工方法对党参饮片质量的影响[J].西部中医药,2019,32(08):20.
　ZHANG Ping,LI Donghua,WANG Xiaolin,et al.Effects of Different Processing Methods and Different Producing Areas on the Quality of Dangshen Decoction Pieces[J].Western Journal of Traditional Chinese Medicine,2019,32(04):20.
[9]贺友安,王冰清,胡辉,等.前胡配方颗粒HPLC含量测定及指纹图谱方法研究[J].西部中医药,2020,33(01):52.[doi:10.12174/j.issn.1004-6852.2020.01.14]
　HE Youan,WANG Bingqing,HU Hui,et al.Research on Fingerprint and Contents Determination of Qianhu Dispensing Granules by HPLC[J].Western Journal of Traditional Chinese Medicine,2020,33(04):52.[doi:10.12174/j.issn.1004-6852.2020.01.14]
[10]张生杰,王丽,田志梅,等.基于一测多评法测定白芍中6种成分含量[J].西部中医药,2022,35(03):44.[doi:10.12174/j.issn.2096-9600.2022.03.10]
　ZHANG Shengjie,WANG Li,TIAN Zhimei,et al.Content Determination of Six Components in Baishao by Quantitative Analysis of Multi-components by Single-marker[J].Western Journal of Traditional Chinese Medicine,2022,35(04):44.[doi:10.12174/j.issn.2096-9600.2022.03.10]

备注/Memo

备注/Memo:
收稿日期：2013-11-20作者简介：王林凤(1973—)，女，主管药师。研究方向：中药质量标准的研究。
更新日期/Last Update: 2014-04-15