[1]鲁梦倩,尼玛次仁,于天源△,等.藏药佐木阿汤对高原红细胞增多症模型大鼠心肌细胞线粒体DNA的影响[J].西部中医药,2016,29(04):10-13.
　LU Mengqian,NIMA Ciren,YU Tianyuan,et al.Influence of Tibetan Medicine ZuoMuA Tang on Myocardial mtDNA of HAPC Rat Model[J].Western Journal of Traditional Chinese Medicine,2016,29(04):10-13.
点击复制

藏药佐木阿汤对高原红细胞增多症模型大鼠心肌细胞线粒体DNA的影响()
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年04期
页码:
10-13
栏目:
出版日期:
2016-04-15

文章信息/Info

Title:
Influence of Tibetan Medicine ZuoMuA Tang on Myocardial mtDNA of HAPC Rat Model
文章编号:
1004-6852(2016)04-0010-04
作者:
鲁梦倩1，尼玛次仁2，于天源1△，冼思彤1，多杰仁青2，潘璠1，巴桑德吉3，张林峰1，陈国勇1，吴剑聪4
1 北京中医药大学，北京 100029； 2 西藏藏医学院； 3 西藏自治区藏医药研究院； 4 北京按摩医院
Author(s):
LU Mengqian1, NIMA Ciren2, YU Tianyuan1△, XIAN Sitong1, DUOJIE Renqing2,PAN Fan1, BASANG Deji3, ZHANG Linfeng1, CHEN Guoyong1, WU Jiancong4
1 Beijing University of Chinese Medicine, Beijing 100029, China; 2 Tibetan Traditional Medical College;3 Tibet Autonomous Region Academy of Tibetan Medicine; 4 Beijing Massage Hospital
关键词:
高原红细胞增多症; 动物实验; 大鼠; 佐木阿汤; 线粒体DNA; 心肌
Keywords:
high altitude polycythemia;  animal experiments;  rats;  ZuoMuA Tang;  mitochondria DNA;  myocardium
分类号:
R285.5
摘要:
目的：观察藏药佐木阿汤对高原红细胞增多症(HAPC)模型大鼠心肌细胞线粒体DNA(mtDNA)及DNA 聚合酶γ等指标表达的影响，探究佐木阿汤对心肌线粒体的保护作用。方法：选用雄性Wistar大鼠32只，随机分为平原对照组、高原模型组、阳性药物组、佐木阿汤组，每组各8只。除平原对照组外，余组均饲养于拉萨以建立HAPC模型，饲养1个月后分别进行生理盐水、红景天和佐木阿汤灌胃干预，干预3个月后取材，采用Realtime PCR、ELISA、Western blot方法分别检测大鼠心肌mtDNA、8-OHdG、DNA聚合酶γ含量。结果：mtDNA含量高原对照组与平原对照组相比，显著降低差异有统计学意义(P＜0.05)；阳性药物组和佐木阿汤组与高原对照组相比，明显升高(P＜0.05)；但与平原对照组相比差异有统计学意义(P＜0.05)。8-OHdG高原对照组与平原对照组相比，显著增高，差异有统计学意义(P＜0.05)；阳性药物组和佐木阿汤组与高原对照组相比，显著降低，差异有统计学意义(P＜0.05)；阳性药物组和佐木阿汤组与平原对照组相比，差异无统计学意义(P＞0.05)。DNA聚合酶γ含量高原对照组与平原对照组相比，显著降低差异有统计学意义(P＜0.05)；阳性药物组和佐木阿汤组与高原对照组相比，差异无统计学意义(P＞0.05)；阳性药物组和佐木阿汤组与平原对照组相比，无明显增加差异无统计学意义(P＞0.05)。结论：佐木阿汤干预HAPC模型大鼠后，心肌mtDNA与DNA聚合酶γ均显著增多，损伤产物8-OHdG显著降低，佐木阿汤可促进线粒体DNA损伤的修复，从而使心肌线粒体的结构和功能逐渐恢复正常，改善HAPC模型大鼠心肌的缺血缺氧状态。
Abstract:
Objective: To explore the mechanism of ZuoMuA Tang protecting myocardial mitochondria by observing its influence on the expressions of mitochondria DNA (mtDNA) and DNA polymerase γ in myocardial cells of rat model with high altitude polycythemia(HAPC). Methods: Thirty-two rats were randomized into plain control group, plateau control group, positive medicine group and ZuoMuA Tang group. Except the rats in plain control group, other rats were kept in Lhasa to prepare HAPC models, and intervened by intragastric administration of physiological saline, HongJingTian(Rhodiola rosea L.) and ZuoMuA Tang after feeding in one months, the tissues were taken after intervened in three months, the contents of myocardial mtDNA, 8-OHdG and DNA polymerase γ of the rats were detected respectively by adopting Realtime PCR, ELISA and Western blot method. Results: The contents of mtDNA decreased notably when plateau control group was compared with plain control group(P＜0.05); they raised obviously when positive medicine group was compared with ZuoMuA Tang group and plateau control group (P＜0.05); but there was the difference when positive medicine group was compared with plain control group (P＜0.05). The levels of 8-OHdG rose significantly when plateau control group was compared with plain control group (P＜0.05); they lowered when positive medicine group was compared with ZuoMuA Tang group and plateau control group(P＜0.05); there was no obvious difference when positive medicine group was compared with ZuoMuA Tang group and plain control group(P＜0.05). The contents of DNA polymerase γ decreased evidently when plateau control group was compared with plain control group(P＜0.05); there was no obvious difference when positive medicine group and ZuoMuA Tang group were compared with plateau control group(P＞0.05); it rose indistinctly when positive medicine group and ZuoMuA Tang group were compared with plain control group(P＞0.05). Conclusion: The levels of myocardial mtDNA and DNA polymerase γ improved significantly, and the levels of damage product 8-OHdG decreased obviously after intervened HAPC rat model by ZuoMuA Tang. ZuoMuA Tang could promote the recovery of mtDNA damage, therefore to make the structure and the functions of myocardial mitochondria restore to normal, and improve ischemic and hypoxia state of HAPC rat model.

相似文献/References:

[1]青格乐图.步长脑心通胶囊治疗高原红细胞增多症40例[J].西部中医药,2012,25(12):63.
　QingGe Le-tu.BuChang NaoXinTong Capsule in the Treatment for 40 Cases of Altitude Erythrocytosis[J].Western Journal of Traditional Chinese Medicine,2012,25(04):63.
[2]丁连翠,路新国.药膳“益脾饮”对脾气虚证大鼠血液流变学及血浆TXB2、6—keto—PGF1α的影响[J].西部中医药,2012,25(05):33.
　DING Lian-cui,LU Xin-guo.Influence of YiPiYin on Hemorheology and TXB2, 6-Keto-PGF1α of Plasma in Rats with Syndrome of Spleen-Qi Deficiency[J].Western Journal of Traditional Chinese Medicine,2012,25(04):33.
[3]燕忠生,李俊,魏千程,等.生脉注射液防治放射性肺炎的实验研究[J].西部中医药,2015,28(04):15.
[4]徐克武,王长慧,强胜林,等.不同补肾方法对骨折大鼠BMP-2表达的影响[J].西部中医药,2013,26(06):15.
　XU Kewu,WANG Changhui,QIANG Shenglin,et al.Effects of Different Kidney-nourishing Methods on Expression of BMP-2 of Rats with Bone Fracture[J].Western Journal of Traditional Chinese Medicine,2013,26(04):15.
[5]潘文,康开彪,王煜,等.清利通淋汤体外和体内抗菌作用的实验研究*[J].西部中医药,2013,26(03):22.
　PAN Wen,KANG Kaibiao,WANG Yu,et al.Experimental Study on Antibacterial Effects of QingLi TongLinTang in Vitro and Vivo[J].Western Journal of Traditional Chinese Medicine,2013,26(04):22.
[6]张晓凤,田耘,程小红,等.肾复康Ⅱ号对MSCs旁分泌作用的实验研究[J].西部中医药,2018,31(10):4.
　ZHANG Xiaofeng,TIAN Yun,CHENG Xiaohong,et al.Experimental Research into ShenFuKang Ⅱin Promoting Paracrine Function of MSCs[J].Western Journal of Traditional Chinese Medicine,2018,31(04):4.
[7]李凯,王育良,张传伟.鬼针草治疗阿托品干眼兔模型的实验研究[J].西部中医药,2017,30(02):13.
　LI Kai,WANG Yuliang,ZHANG Chuanwei.Experimental study on Sticktight in Treating Atropine Dry Eye Rabbit Model[J].Western Journal of Traditional Chinese Medicine,2017,30(04):13.
[8]梁曦,薛建军△.甘草喷雾剂对大鼠全麻气管插管后呼吸道黏膜及细胞因子的影响[J].西部中医药,2017,30(02):16.
　LIANG Xi,XUE Jianjun.Effects of Licorice Spray on Respiratory Tract Mucosa and Cytokines of Rats after General Anesthesia Tracheal Intubation[J].Western Journal of Traditional Chinese Medicine,2017,30(04):16.
[9]潘文,康开彪,李淑玲,等.归甲疏通胶囊对大鼠离体子宫平滑肌收缩的影响[J].西部中医药,2017,30(02):20.
　PAN Wen,KANG Kaibiao,LI Shuling,et al.Effects of GuiJiaShuTong Capsule on Contractility of Rat Uterine Smooth Muscle in Vitro[J].Western Journal of Traditional Chinese Medicine,2017,30(04):20.
[10]陈丽如,刘源,聂玮,等.柴芪汤对代谢综合征模型大鼠血清TNF-α及IL-6水平的影响[J].西部中医药,2016,29(04):6.
　CHEN Liru,LIU Yuan,NIE Wei,et al.The Influence of ChaiQi Tang on the Levels of TNF-α and IL-6 of the Rat Model with Metabolic Syndrome[J].Western Journal of Traditional Chinese Medicine,2016,29(04):6.

备注/Memo

备注/Memo:
收稿日期：2015-11-09*基金项目：国家自然科学基金项目(编号81260567)。作者简介：鲁梦倩(1987—)，女，博士研究生。研究方向：针灸推拿治疗周围神经损伤的机理研究。△通讯作者：于天源(1965—)，男，博士学位，教授。研究方向：针灸推拿治疗周围神经损伤的机理研究。
更新日期/Last Update: 2016-04-15