[1]刘帅,曹保利.莪术油对大鼠子宫内膜异位症基质细胞衍生因子-1及其受体表达的影响[J].西部中医药,2018,31(03):26-29.
　LIU Shuai,CAO Baoli.The Effects of Curcuma Zedoary Oil on the Expressions of Stromal Cell Derived Factor-1 and Its Receptor in Endometriosis Rats Model[J].Western Journal of Traditional Chinese Medicine,2018,31(03):26-29.
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莪术油对大鼠子宫内膜异位症基质细胞衍生因子-1及其受体表达的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年03期
页码:
26-29
栏目:
出版日期:
2018-03-15

文章信息/Info

Title:
The Effects of Curcuma Zedoary Oil on the Expressions of Stromal Cell Derived Factor-1 and Its Receptor in Endometriosis Rats Model
文章编号:
1004-6852(2018)03-0026-04
作者:
刘帅1，曹保利2
1 天津中医药大学，天津 300193； 2 天津市南开医院妇产科
Author(s):
LIU Shuai1, CAO Baoli 2
1 Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;2 Department of Gynecology and Obstetrics, Tianjin Nankai Hospital
关键词:
子宫内膜异位症; SDF-1; CXCR4; 莪术油; 大鼠
Keywords:
endometriosis;  SDF-1;  CXCR4;  curcuma zedoary oil;  rats
分类号:
R285.5
摘要:
目的：观察莪术油对子宫内膜异位症模型大鼠异位内膜组织中基质细胞衍生因子-1(SDF-1)及其趋化因子受体4(CXCR4)表达的影响。方法：采用自体移植法建立非动情期SD大鼠子宫内膜异位症模型，将造模成功的32只大鼠随机分为模型组、莪术油(低、中、高剂量)组，每组8只，另设8只健康大鼠作为空白组；连续给药28天后观察异位内膜生长情况，切取在位及异位内膜组织，行PCR法检测大鼠在位、异位内膜组织及正常在位子宫内膜组织SDF-1和CXCR4的mRNA表达情况，对比莪术油各剂量组与模型组异位内膜组织中SDF-1 mRNA，CXCR4 mRNA的表达差异。结果：大鼠异位内膜组织的SDF-1 mRNA和CXCR4 mRNA高度表达，与在位内膜组织及正常子宫内膜组织比较差异有统计学意义(P＜0.05)；莪术油各剂量组异位内膜组织SDF-1mRNA表达均有不同程度下降，且各剂量组间比较差异有统计学意义(P＜0.05)；莪术油各剂量组CXCR4 mRNA表达亦有下降趋势，各剂量组间比较差异有统计学意义(P＜0.05)；莪术油各剂量组SDF-1 mRNA和CXCR4 mRNA的表达均显著低于模型组。结论：SDF-1/CXCR4轴可能参与了子宫内膜异位症的发展过程；莪术油可明显降低异位内膜组织中SDF-1、CXCR4表达，对子宫内膜异位症起一定的治疗作用。
Abstract:
Objective: To investigate the influence of curcuma zedoary oil on stromal cell derived factor-1 (SDF-1) and its receptor CXC chemokine receptor(CXCR4) expressions in endometriosis (EM) rats model. Methods: The EM rat models were established by self-endometrium transplantation during non-estrus period and randomly assigned into the model group, the high-dose group, the middle-dose group and the low-dose group (eight rats in each group), eight healthy rats were taken as the blank group. After 28 days of successive administration, the growth of ectopic endometria was observed; the eutopic and ectopic endometria were removed in preparation for PCR method in test of the expression of SDF-1 and CXCR4 mRNA, to obtain the difference between the treatment group and the control group. Results: SDF-1 and CXCR4 mRNA were highly expressed in ectopic endometrial tissue, there is astatistical difference in comparison with eutopic endometria and normal endometria tissue (P＜0.05); the expressions of SDF-1 mRNA in ectopic endometrial tissue declined in curcuma zedoary oil groups (low, middle and high doses) to different degrees, and the differences had statistical meaning among different doses groups (P＜0.05); the expressions of CXCR4mRNA showed the tendency of declining in curcuma zedoary oil groups (low, middle and high doses), and the difference had statistical meaning among different doses groups (P＜0.05); curcuma zedoary oil groups (low, middle and high doses) were lower than the model group in the expressions of SDF-1 mRNA and CXCR4 mRNA. Conclusion: SDF-1/CXCR4 axis might participate in the developmental process of endometriosis; curcuma zedoary oil could lower the expressions of SDF-1 and CXCR4 in ectopic endometrial tissue and it shows certain therapeutic effects in treating EM.

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备注/Memo

备注/Memo:
收稿日期：2017-05-19 作者简介：刘帅(1990—)，男，在读硕士研究生。研究方向：妇科疾病的中西医结合治疗。
更新日期/Last Update: 2018-03-15