[1]曹团武,赵理,罗浩,等.表儿茶素为指标的金荞麦有效成分提取纯化工艺研究[J].西部中医药,2019,32(03):36-40.
　CAO Tuanwu,ZHAO Li,LUO Hao,et al.Study on the Extraction and Purification Technology for Active Ingredients of JinQiaoMai by Taking Epicatechin as the Marker[J].Western Journal of Traditional Chinese Medicine,2019,32(03):36-40.
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表儿茶素为指标的金荞麦有效成分提取纯化工艺研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年03期
页码:
36-40
栏目:
出版日期:
2019-03-15

文章信息/Info

Title:
Study on the Extraction and Purification Technology for Active Ingredients of JinQiaoMai by Taking Epicatechin as the Marker
文章编号:
1004-6852(2019)03-0036-05
作者:
曹团武，赵理，罗浩，时建伟，蒋勇
长江师范学院化学化工学院/武陵山天然药物研究与开发实验室，重庆 408100
Author(s):
CAO Tuanwu, ZHAO Li, LUO Hao, SHI Jianwei, JIANG Yong
School of Chemistry and Chemistry Engineering, Yangtze Normal University/Wulingshan Lab for Natural Product Research and Development, Fuling 408100, China
关键词:
金荞麦; 有效成分; 表儿茶素; 分离纯化; 大孔树脂
Keywords:
JinQiaoMai;  active ingredients;  epicatechin;  separation and purification;  macroporous resin
分类号:
R284
摘要:
目的：优选金荞麦有效成分的提取纯化工艺。方法：以表儿茶素(Epicatechin)的提取率、吸附率和解吸附率为指标，结合HPLC含量测定，选择最佳提取条件、树脂型号和纯化工艺；同时考察乙酸乙酯和正丁醇的纯化效果。结果：最佳超声提取工艺条件为乙醇浓度50%，液料比1∶10，提取3次，30 min/次；最佳纯化工艺为上样液浓度0.5 g/mL，pH5～6，以3 BV/h流过D101大孔树脂，流出液重复上柱2次，加4 BV水洗脱除杂，加3 BV 50%乙醇洗脱富集有效成分；提取液中有效成分也可用正丁醇萃取富集。结论：采用乙醇超声提取，D101大孔树脂分离纯化或正丁醇纯化金荞麦有效成分的工艺方法稳定、可行。
Abstract:
Objective: To optimize the extraction and purification technology for the active ingredients of JinQiaoMai[Fagopyrum dibotrys (D. Don) Hara]. Methods: The purification technology, the types of resins and the best extraction conditions were chosen by taking the extraction rate, absorption rate and desorption rate of epicatechin as the indexes combined with content determination by HPLC. Results: The best ultrasonic extraction conditions were the concentrations of ethanol 50%, liquid-material ratio was one to ten, extraction times were three times, 30 min each time; the best purification technology：the concentrations of sample solution were 0.5 g/mL, pH of 5 to 6, absorbed by macroporous resin with a flow rate of 3 BV/h, effluent liquid on column twice repeatedly, adding 4BV of water to elute and remove impurities, adding 3 BV of 50% ethanol to elute the enriched active ingredients; the active ingredients in the extract could be leached and enriched by n-butyl alcohol. Conclusion: Active ingredients of JinQiaoMai are extracted by ethanol ultrasonic extraction, separated and purified by D101 macroporous resin or purified by n-butyl alcohol, and the technology method is stable and feasible.

参考文献/References:

A

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备注/Memo

备注/Memo:
收稿日期：2018-02-15 *基金项目：涪陵区科技计划项目(编号FLKJ，2015ABA1018)；长江师范学院科研创新平台建设项目(编号2015XJPT01)；长江师范学院青年科研人才项目（编号2016QNRC02）。 作者简介：曹团武(1977—)，男，博士学位，副教授。研究方向：天然药物药效物质基础、中药质量综合评价。
更新日期/Last Update: 2019-03-15