[1]顿珠次仁,陈正红,米玛,等.关于藏药矮紫堇有效成分及色谱图的研究[J].西部中医药,2020,33(04):55-58.[doi:10.12174/j.issn.1004-6852.2020.04.17]
　Dunzhu Ciren,CHEN Zhenghong,MI Ma,et al.Research on the Chromatogram and Active Ingredients of Tibetan Medicine Aizijin[J].Western Journal of Traditional Chinese Medicine,2020,33(04):55-58.[doi:10.12174/j.issn.1004-6852.2020.04.17]
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关于藏药矮紫堇有效成分及色谱图的研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年04期
页码:
55-58
栏目:
出版日期:
2020-04-15

文章信息/Info

Title:
Research on the Chromatogram and Active Ingredients of Tibetan Medicine Aizijin
文章编号:
1004-6852(2020)04-0055-04
作者:
顿珠次仁，陈正红，米玛，靳贵林
西藏藏医学院，西藏 拉萨 850000
Author(s):
Dunzhu Ciren, CHEN Zhenghong, MI Ma, JIN Guilin△
Tibetan Traditional Medical College, Lasa 850000, China
关键词:
矮紫堇; 液相色谱; 原阿片碱; 含量; 色谱图
Keywords:
Aizijin;  liquid chromatography;  protopine;  contents;  chromatography
分类号:
R285.5
DOI:
10.12174/j.issn.1004-6852.2020.04.17
文献标志码:
A
摘要:
目的：以原阿片碱为指标性成分建立矮紫堇质量评价的液相色谱方法。方法：用乙腈-0.3%磷酸梯度洗脱，0～5 min乙腈15%、5～10 min乙腈20%、10～20 min乙腈22%、20～30 min乙腈23%、30～35 min乙腈25%、35～40 min乙腈26%、40～45 min乙腈27%、45～55 min乙腈32%、55～60 min乙腈55%、60～70 min乙腈65%、70～75 min乙腈5%，75～80 min乙腈5%；检测波长289 nm、350 nm，进样量10 μL测定原阿片碱含量。结果：矮紫堇中原阿片碱含量在(1.715±0.041)mg/g范围内波动，原阿片碱在289 nm波长下出峰，在350 nm波长下不出峰。结论：原阿片碱在289 nm处出峰较好，在350 nm处不出峰，可以较为方便点的识别矮紫堇中原阿片碱的峰位；以原阿片碱含量测定简单方便，可以作为矮紫堇用药安全的指标成分。
Abstract:
Objective: To set up the method of liquid chromatography for quality evaluation of Aizijin (herba corydalis) by taking protopine as the indicator component. Methods: Acetonitrile-0.3% phosphoric acid solution were used as gradient elution, 0 - 5min acetonitrile 15%, 5 - 10 min acetonitrile 20%, 10 - 20 min acetonitrile 22%, 20 - 30 min acetonitrile 23%, 30 - 35 min acetonitrile 25%, 35 - 40 min acetonitrile 26%, 40 - 45 min acetonitrile 27%, 45 - 55 min acetonitrile 32%, 55 - 60 min acetonitrile 55%, 60 - 70 min acetonitrile 65%, 70 - 75 min acetonitrile 5%, 75 - 80 min acetonitrile 5%; detection wavelength 289 nm and 350 nm, sample size 10 μL, to detect the contents of protopine. Results: The contents of protopine contained in Aizijin fluctuated within the range of (1.715 ±0.041) mg/g, protopine peaked at the wavelength of 289 nm and didn′t peak at 350 nm. Conclusion: Protopine could reach the peak at 289 nm and won′t peak at 350 nm, and it could help identify the peak of protopine contained in Aizijin; simple and convenient determination of the contents of protopine could be used as the indicator component for drug safety of Aizijin.

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[2]靳贵林,米玛,杨春艳,等.藏药“矮紫堇”中有效成分含量测定及安全用药指导研究[J].西部中医药,2020,33(05):67.[doi:10.12174/j.issn.1004-6852.2020.05.19]
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备注/Memo

备注/Memo:
收稿日期：2019-07-04*基金项目：2016年西藏自治区高校青年教师创新支持计划项目（QCZ2016-79）。作者简介：顿珠次仁(1982—)，男，硕士学位，副教授。研究方向：药物化学物分析。△通讯作者：靳贵林(1989—)，男，讲师，硕士学位，副主任医师。研究方向：中药品质及中药材规范化栽培研究。
更新日期/Last Update: 2020-04-15