[1]胡金霞,杨帆,卢军,等.景衣安神散穴位贴敷对失眠大鼠行为学及相关细胞因子的影响?[J].西部中医药,2021,34(11):41-45.[doi:10.12174/j.issn.2096-9600.2021.11.09]
　HU Jinxia,YANG Fan,LU Jun,et al.Effects of Jingyi Anshen Powder Acupoint Application on Behavior and the Related Cytokines in Rats with Insomnia[J].Western Journal of Traditional Chinese Medicine,2021,34(11):41-45.[doi:10.12174/j.issn.2096-9600.2021.11.09]
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景衣安神散穴位贴敷对失眠大鼠行为学及相关细胞因子的影响?
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年11期
页码:
41-45
栏目:
出版日期:
2021-11-15

文章信息/Info

Title:
Effects of Jingyi Anshen Powder Acupoint Application on Behavior and the Related Cytokines in Rats with Insomnia
作者:
胡金霞1, 杨帆2, 卢军1, 王洪霞1, 江钰1, 刘涛1, 胡晓灵1
1.新疆维吾尔自治区中医药研究院，新疆 乌鲁木齐 830000
2.新疆军区总医院健康管理科
Author(s):
HU Jinxia1, YANG Fan2, LU Jun1, WANG Hongxia1, JIANG Yu1, LIU Tao1, HU Xiaoling1
1.Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine, Urumqi 830000, China
2.Department of Health Management, General Hospital of Xinjiang Military Region
关键词:
失眠; 行为学; 细胞因子; 穴位贴敷; 景衣安神散; 动物实验
Keywords:
insomnia; behavior; cytokine; acupoint application; powder; zoopery
分类号:
R282
DOI:
10.12174/j.issn.2096-9600.2021.11.09
文献标志码:
A
摘要:
目的探讨景衣安神散穴位贴敷对失眠大鼠行为学以及细胞因子的影响。 方法将雄性大鼠64只按随机数字表法分为对照组、实验组、模型组、空白组，每组16只。对照组腹腔注射0.9%氯化钠溶液（1 mL/100 g），每日1次，连续注射2天，饲养7天。实验组、模型组、空白组均腹腔注射对苯丙氨酸（PCPA），每日1次，连续注射2天制造睡眠剥夺模型。造模后模型组不进行任何干预，连续饲养7天。空白组将空白敷膜贴于神阙、内关穴，每天1次，每次30 min，连续7天。实验组将景衣安神散敷贴于神阙、内关穴，每天1次，每次30 min，连续7天。实验第8天采用酶联免疫吸附法（enzyme-linked immunosorbent assay,ELISA）检测大鼠血清白细胞介素1β(interleukin-1β，IL-1β)及肿瘤坏死因子α(tumor necrosis factor-α，TNF-α)含量。 结果实验组大鼠空旷实验得分下降，中央格停留时间减少，粪便颗粒减少（P＜0.01），修饰次数增多（P＜0.01），大鼠挣扎次数较模型组减少，较空白组增加（P＜0.01），绝望时间较模型组缩短，较空白组延长（P＜0.01），实验组大鼠血清IL-1β及TNF-α逐渐升高，差异有统计学意义（P＜0.01）。 结论景衣安神散穴位贴敷可以升高失眠大鼠血清IL-1β，TNF-α含量，诱导睡眠，恢复正常的睡眠-觉醒周期。
Abstract:
ObjectiveTo explore the effects of Jingyi Anshen powder acupoint application on the behavior and the related cytokines in rats with insomnia. MethodsAll 64 male rats were divided into the control group, the experiment group, the model group and the blank group according to random number table, 16 rats in each group. The control group accepted intraperitoneal injection of 0.9% sodium chloride solution (1 mL/100 g), once each day, for two consecutive days, feeding for seven days. The experiment group, the model group and the blank group received intraperitoneal injection of PCPA, once each day, for two consecutive days to set up sleep-deprivation models. After modelling, no intervention was given to the model group, and they were raised for seven consecutive days. The blank group applied blank film to Shenque (RN8) and Neiguan (PC6), once each day, 30 minutes each time, for seven days consecutively. The experiment group applied Jingyi Anshen powder to Shenque (RN8) and Neiguan (PC6), once each day, 30 minutes each time, for seven days consecutively. On the 8th day of the experiment, ELISA method was used to detect the contents of IL-1β and TNF-α of the rats. ResultsThe scores of open experiment decreased, the residence time of the central cell reduced, and fecal particles cut in the experiment group (P<0.01), grooming times increased (P<0.01), the number of struggles in rats reduced than the model group, in creased than the blank group (P<0.01), desperate time shortened than the model group, prolonged than the blank group (P<0.01), the levels of IL-1β and TNF-α rose in the experiment group, and the difference had statistical meaning (P<0.01). ConclusionJingyi Anshen powder acupoint application could lift the contents of IL-1β and TNF-α in the rats with insomnia, induce sleep, and return to normal sleep wake cycle.

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备注/Memo

备注/Memo:
胡金霞（1974—），女，硕士学位，主任医师，第六批全国老中医药专家学术经验继承人。研究方向：老年病的中西医结合临床与研究。新疆维吾尔自治区自然科学基金项目（2017D01C160）。
更新日期/Last Update: 2022-08-09