[1]张素,邵阳,华臻,等.脊髓康对大鼠脊髓损伤后胶质瘢痕形成及硫酸软骨素蛋白多糖表达的影响[J].西部中医药,2022,35(08):26-29.[doi:10.12174/j.issn.2096-9600.2022.08.07]
　ZHANG Su,SHAO Yang,HUA Zhen,et al.Impacts of Jisuikang on Gelatinous Scar Formation and the Expressions of Chondroitin Sulfate Proteoglycans in Rats after Spinal Cord Injury[J].Western Journal of Traditional Chinese Medicine,2022,35(08):26-29.[doi:10.12174/j.issn.2096-9600.2022.08.07]
点击复制

脊髓康对大鼠脊髓损伤后胶质瘢痕形成及硫酸软骨素蛋白多糖表达的影响
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年08期
页码:
26-29
栏目:
出版日期:
2022-08-15

文章信息/Info

Title:
Impacts of Jisuikang on Gelatinous Scar Formation and the Expressions of Chondroitin Sulfate Proteoglycans in Rats after Spinal Cord Injury
作者:
张素1, 邵阳2, 华臻2, 杨俊锋2, 王建伟1,2
1.南京中医药大学，江苏 南京 210023
2.南京中医药大学无锡附属医院
Author(s):
ZHANG Su1, SHAO Yang2, HUA Zhen2, YANG Junfeng2, WANG Jianwei1,2
1.Nanjing University of Chinese Medicine,Nanjing 210023,China
2.Wuxi Traditional Chinese Medicine Hospital
关键词:
脊髓损伤; 脊髓康; 硫酸软骨素蛋白多糖; 胶质瘢痕; 动物实验; 大鼠
Keywords:
spinal cord injury; chondroitin sulfate proteoglycans; gelatinous scar; animal experiments; rats
分类号:
R651.2
DOI:
10.12174/j.issn.2096-9600.2022.08.07
摘要:
目的研究脊髓康对大鼠脊髓损伤区胶质瘢痕形成及硫酸软骨素蛋白聚糖（chondroitin sulfate proteoglycans，CSPG）表达的抑制作用。 方法将60只SD大鼠随机分为正常对照组、模型组、脊髓康组及激素组，每组15只。除正常对照组外，其余3组利用改良Allen法制造脊髓损伤大鼠模型，各组给予对应药物治疗。于第3、7、14天抽取样本制备切片标本，通过HE染色与EnVision法染色观察各组大鼠脊髓受损区的病理形态学改变；实时荧光定量PCR检测各组脊髓损伤区CSPG表达水平。 结果在造模后第3、7、14天，脊髓康组和激素组相比于模型组，病理图片中脊髓损伤区域内胶质瘢痕形成更少，且CSPG表达受到明显抑制（P＜0.05），同时，脊髓康组相比于激素组，病理图片中脊髓损伤区域内胶质瘢痕形成差异不明显，但CSPG表达量高于激素组（P＜0.05）。 结论中药脊髓康可以抑制大鼠脊髓损伤区胶质瘢痕过度形成及CSPG的表达，对于促进脊髓损伤后神经修复再生具有积极意义。
Abstract:
ObjectiveTo study the inhibitory effects of Jisuikang on gelatinous scar formation and the expressions of chondroitin sulfate proteoglycans (CSPG) in rats after spinal cord injury. MethodsSixty SD rats were randomized into normal control group, the model group, Jisuikang group and hormone group, with 15 rats in each group. Except normal control group, the other three groups were established into rat models by using imp-roved Allen method, different groups were treated by the corresponding medicine. Section preparation was performed after samples were selected at the third, seventh and fourteenth day, pathological morphological changes in the area of spinal cord injury of the rats were observed in different groups after HE staining and EnVision method; the expression of CSPG in the area of spinal cord injury were measured by using real-time fluorescence quantitative PCR. ResultsAt the third, seventh and 14th day after modeling, the formation of gelatinous scar was less in the area of spinal cord injury in the pathological images, and the expressions of CSPG were inhibited obviously when Jisuikang group and hormone group were compared with the model group (P<0.05), meanwhile, the difference was insignificant in the formation of gelatinous scar in the area of spinal cord injury in the pathological images when Jisuikang group was compared with hormone group, while the expressions of CSPG were higher than these of hormone group (P<0.05). ConclusionJisuikang could control excessive formation of gelatinous scar and the expressions of CSPG in rats after spinal cord injury, which has a positive meaning for promoting nerve repair regeneration after spinal cord injury.

相似文献/References:

[1]赵道洲,张绍文,周红.脊髓损伤后神经细胞凋亡相关抑制蛋白表达的实验研究[J].西部中医药,2015,28(12):18.
[2]邢涛,宋焱峰,董林,等.凝血酶对脊髓组织毒性损伤机制及水蛭素的干预作用[J].西部中医药,2011,24(11):7.
[3]陆凤连.腹部八卦推拿手法对脊髓损伤便秘患者排便的影响[J].西部中医药,2014,27(06):152.
　LV Fenglian.Effects of Abdominal Eight Diagrams Manipulation Maneuver on Bowel Movement of Constipation Patients with Spinal Injury[J].Western Journal of Traditional Chinese Medicine,2014,27(08):152.
[4]徐秀梅,徐彦龙,寄婧,等.针灸结合康复训练治疗脊髓损伤患者神经源性膀胱疗效观察[J].西部中医药,2018,31(05):97.
　XU Xiumei,XU Yanlong,JI Jing,et al.Clinical Observation on Acupuncture Combined with Rehabilitation Training on Neurogenic Bladder of the Patients with Spinal Injury[J].Western Journal of Traditional Chinese Medicine,2018,31(08):97.
[5]池伟东,庄娟娜,吴俊哲△.基于中医传承辅助系统的脊髓损伤中药处方规律研究[J].西部中医药,2019,32(04):61.
　CHI Weidong,ZHUANG Juanna,WU Junzhe.Research on the Rules of Herbal Prescription for Spinal Injury Based on TCMISS[J].Western Journal of Traditional Chinese Medicine,2019,32(08):61.
[6]杨小霞,于慧金,牛见升,等.参附注射液治疗高位脊髓损伤后体位性低血压的疗效观察[J].西部中医药,2021,34(06):92.[doi:10.12174/j.issn.2096-9600.2021.06.22]
　YANG Xiaoxia,YU Huijin,NIU Jiansheng,et al.Observation on Clinical Effects of Shenfu Injection in Treating Postural Hypotension after Spinal Cord Injury[J].Western Journal of Traditional Chinese Medicine,2021,34(08):92.[doi:10.12174/j.issn.2096-9600.2021.06.22]
[7]杨小霞,于慧金,孙军弟,等.中医药治疗脊髓损伤尿失禁的卫生经济学评价[J].西部中医药,2023,36(03):86.[doi:10.12174/j.issn.2096-9600.2023.03.16]
　YANG Xiaoxia,YU Huijing,SUN Jundi,et al.Health Economic Evaluation of Treating Urinary Incontinence in Spinal Cord Injury by TCM Therapy[J].Western Journal of Traditional Chinese Medicine,2023,36(08):86.[doi:10.12174/j.issn.2096-9600.2023.03.16]
[8]罗颖慧,王琼,欧阳梦婷,等.不同时间针灸治疗联合膀胱再训练模式对脊髓损伤后神经源性膀胱的价值分析[J].西部中医药,2024,37(04):145.[doi:10.12174/j.issn.2096-9600.2024.04.31]
　LUO Yinghui,WANG Qiong,OUYANG Mengting,et al.The Value of Acupuncture at Different Times Combined with Bladder Retraining Mode on Neurogenic Bladder after Spinal Cord Injury[J].Western Journal of Traditional Chinese Medicine,2024,37(08):145.[doi:10.12174/j.issn.2096-9600.2024.04.31]
[9]徐秀梅,徐彦龙,米登海.“三调针法”治疗脊髓损伤后神经源性膀胱临床观察[J].西部中医药,2024,37(08):153.[doi:10.12174/j.issn.2096-9600.2024.08.35]
　XU Xiumei,XU Yanlong,MI Denghai.Clinical Observation on the Treatment of Neurogenic Bladder after Spinal Cord Injury by "Three Modulation Acupuncture"[J].Western Journal of Traditional Chinese Medicine,2024,37(08):153.[doi:10.12174/j.issn.2096-9600.2024.08.35]

备注/Memo

备注/Memo:
张素（1991—），男，硕士学位，医师。研究方向：创伤与关节病的研究。无锡市临床医学中心科研项目（LCZXJS003）。
更新日期/Last Update: 2022-08-15