[1]曾顺,刘楠楠,徐波,等.安神定志丸及拆方对睡眠剥夺小鼠学习记忆及cAMP-CREB-BDNF信号通路蛋白表达的影响[J].西部中医药,2022,35(12):19-24.[doi:10.12174/j.issn.2096-9600.2022.12.05]
　ZENG Shun,LIU Nannan,XU Bo,et al.Effects of Anshen Dingzhi Pills and Its Compatibility on the Expressions of cAMP-CREB-BDNF Signaling Pathway Protein, Learning and Memory Ability in Sleep Deprivation Mice[J].Western Journal of Traditional Chinese Medicine,2022,35(12):19-24.[doi:10.12174/j.issn.2096-9600.2022.12.05]
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安神定志丸及拆方对睡眠剥夺小鼠学习记忆及cAMP-CREB-BDNF信号通路蛋白表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年12期
页码:
19-24
栏目:
理论论著
出版日期:
2022-12-15

文章信息/Info

Title:
Effects of Anshen Dingzhi Pills and Its Compatibility on the Expressions of cAMP-CREB-BDNF Signaling Pathway Protein, Learning and Memory Ability in Sleep Deprivation Mice
作者:
曾顺1, 刘楠楠1, 徐波2, 丁莉2
1.十堰市太和医院，湖北 武汉 430065
2.湖北中医药大学
Author(s):
ZENG Shun1, LIU Nannan1, XU Bo2, DING Li2
1.Taihe Hospital, Wuhan 430065, China
2.Hubei University of Chinese Medicine
关键词:
睡眠剥夺; 学习记忆; 安神定志丸; 神经因子; 小鼠; 动物实验
Keywords:
sleep deprivation; learning and memory ability; pills; neural factors; mice; animal experiment
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2022.12.05
文献标志码:
A
摘要:
目的探讨安神定志丸及拆方对睡眠剥夺（sleep deprivation，SD）模型小鼠学习记忆水平及cAMP-CREB-BDNF蛋白表达的影响。 方法选取雄性昆明小鼠60只为研究对象，通过组方分析主要由安神药和化痰药组成，故拆方分为宁心安神组、补气化痰组，空白组、模型组、安神定志（原方）组和艾司唑仑组，每组10只。采用腹腔注射对氯苯丙氨酸（parachlorophenylalanine，PCPA）混悬液制备睡眠剥夺模型。造模后采用Morris水迷宫检测学习记忆水平；免疫组织化学法（immunohistochemistry，IHC）计算海马磷酸化环磷腺苷效应元件结合蛋白（p-cAMP-response element binding protein，p-CREB）、脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）阳性细胞表达；尼氏染色观察SCN神经元尼氏小体变化；酶联免疫吸附法（ELISA）检测血清环磷酸腺苷（Cyclic adenosine monophosphate，cAMP）、p-CREB、BDNF的浓度，蛋白质印迹法（Western blotting）检测海马中p-CREB、BDNF的蛋白水平。 结果与模型组比较，安神定志组上平台潜伏期、游泳总路程、第一次抵达平台时间缩短，跨越平台次数、目标象限时间增加，SCN神经元尼氏小体增加，cAMP、p-CREB、BDNF蛋白表达升高（P＜0.01）。拆方结果显示，补气化痰组和宁心安神组均能够改善SD小鼠定位航行与空间探索能力，增加cAMP、p-CREB、BDNF蛋白表达，两组间比较差异有统计学意义（P＜0.01，P＜0.05），与安神定志组比较同时具有显著性差异（P＜0.01，P＜0.05）。 结论安神定志丸改善SD小鼠的学习记忆水平，与调节cAMP-CREB-BDNF信号通路蛋白表达有关，各组间影响程度依次为安神定志组>补气化痰组>宁心安神组。
Abstract:
ObjectiveTo explore the impacts of Anshen Dingzhi pills and its compatibility on the express-ions of cAMP-CREB-BDNF signaling pathway protein, learning and memory ability in sleep deprivation (SD) mice. MethodsSixty male Kunming mices were selected as the research objects, and divided into the groups of calming the heart and tranquilizing the mind, tonifying Qi and reducing phlegm, the blank group, the model group, Anshen Dingzhi (the original prescription) group and estazolam group, since the prescription is composed of the herbs of tranquilizing the mind and reducing phlegm, with ten mice in each group. Intraperitoneal injection of PCPA suspension was used to establish SD models. After modeling, Morris water maze was adopted to detect learning and memory ability; IHC was applied to calculate the expressions of hippocampal p-CREB, and positive cells of BDNF; nissl's staining used to observe the changes of SCN neuron nissl bodies; ELISA method to detect the density of cAMP, p-CREB and BDNF, Western blotting to measure the levels of p-CREB and BDNF protein. ResultsCompared with the model group, the incubation period of hopping on platform, total swimming distances, and the first arrival time on platform shortened in Anshen Dingzhi group, the number of crossing platforms, and target quadrant time increased, SCN neuron nissl bodies added, the expressions of cAMP, p-CREB and BDNF elevated (P<0.01). The results of the compatibility showed that the groups of calming the heart and tranquilizing the mind, tonifying Qi and reducing phlegm could improve place navigation and space exploration capability of SD mice, raise the expressions of cAMP, p-CREB and BDNF, and the difference had statistical meaning between both groups (P<0.01, P<0.05). ConclusionAnshen Dingzhi pills could improve SD mice's learning and memory ability, connected with regulating the expressions of cAMP-CREB-BDNF signaling pathway protein, the groups rank according to the degree of influence: Anshen Dingzhi group> tonifying Qi and reducing phlegm group>group of calming the heart and tranquilizing the mind.

相似文献/References:

[1]肖迪,刘俊△,赵宾宾.酸枣仁汤对慢性睡眠剥夺大鼠海马5-HT1AR和5-HT2AR表达的影响[J].西部中医药,2019,32(05):12.
　XIAO Di,LIU Jun,ZHAO Binbin.Impacts of SuanZaoRen Decoction on the Expressions of 5-HT1AR and 5-HT2AR in Hippocampus of the Rats with Chronic Sleep Deprivation[J].Western Journal of Traditional Chinese Medicine,2019,32(12):12.

备注/Memo

备注/Memo:
曾顺（1990—），男，硕士学位。研究方向：中医药防治神经系统疾病的机理研究。国家自然科学基金青年基金（81703972）。
更新日期/Last Update: 2022-12-15