[1]陈广芳,文昌晖.基于GEO芯片探究槲皮素对银屑病外周血中性粒细胞的分子作用机制[J].西部中医药,2023,36(09):25-34.[doi:10.12174/j.issn.2096-9600.2023.09.06]
　CHEN Guangfang,WEN Changhui.Molecular Mechanism of Intervention Effects of Quercetin on Neutrophile Granulocyte in Peripheral Blood of Psoriasis Based on GEO Chip[J].Western Journal of Traditional Chinese Medicine,2023,36(09):25-34.[doi:10.12174/j.issn.2096-9600.2023.09.06]
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基于GEO芯片探究槲皮素对银屑病外周血中性粒细胞的分子作用机制
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年09期
页码:
25-34
栏目:
出版日期:
2023-09-15

文章信息/Info

Title:
Molecular Mechanism of Intervention Effects of Quercetin on Neutrophile Granulocyte in Peripheral Blood of Psoriasis Based on GEO Chip
作者:
陈广芳1,2, 文昌晖2
1.贵州中医药大学第一临床医学院，贵州 贵阳 550002
2.贵州中医药大学第一附属医院，贵州 贵阳 550002
Author(s):
CHEN Guangfang1,2, WEN Changhui2
1.First Clinical Medical College, Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China
2.First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550002, China
关键词:
银屑病; 基因表达数据库; 中性粒细胞; 槲皮素; 分子对接; JUN; SSP1; IL-17信号通路; TNF信号通路
Keywords:
psoriasis; GEO; neutrophile granulocyte; quercetin; molecular docking; JUN; SSP1; IL-17 signaling pathway; TNF signal pathway
分类号:
R223.1+4
DOI:
10.12174/j.issn.2096-9600.2023.09.06
文献标志码:
A
摘要:
目的通过基因表达数据库（GEO）芯片分析及分子对接方法探讨槲皮素对银屑病外周血中性粒细胞弹性蛋白酶（neutrophil elastase，NE）的分子机制。 方法从公共GEO下载GSE106087芯片数据，筛选差异靶点基因；收集中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）中槲皮素预测靶点基因，与银屑病外周血NE差异性基因取交集，收集槲皮素干预银屑病外周血NE的靶标基因，并进行String平台验证，同时进行基因本体论功能富集分析（gene ontology，GO）和KEGG富集分析（kyoto encyclopedia of genes and genomes，KEGG），最后通过分子对接证据支持分析槲皮素干预银屑病NE的作用机制。 结果1）GSE106087基因芯片共包含基因49454个，银屑病外周血NE差异性基因189个（上调36个、下调153个）；GO富集分析为432条生物过程、23条细胞成分、6条分子功能；KEGG富集分析为12条信号通路；2）槲皮素干预银屑病外周血NE差异基因11个（下调7个、上调4个），拓扑网络筛选核心靶标基因为JUN、SSP1（均为银屑病外周血NE上调基因），通过String平台验证为8个，其中能与槲皮素进行分子对接基因共6个，GO富集分析生物过程共291条、细胞成分7个、分子功能14个；KEGG富集分析为34条信号通路。 结论1）GSE106087基因芯片包含基因49454个，银屑病外周血NE的病理机制主要与ARG1等基因、中性粒细胞活化、病毒蛋白与细胞因子、细胞因子受体相互作用等上调，以及SPP1等基因、中性粒细胞脱颗粒生物过程、NOD样受体信号通路等下调关系密切；2）槲皮素干预银屑病外周血NE机制，主要与上调JUN、SSP1和下调IL-17信号通路、TNF信号通路等密切相关。
Abstract:
ObjectiveTo study molecular mechanism of the intervention effects of quercetin on NE in peripheral blood of psoriasis patients through GEO chip analysis and molecular docking method. MethodsGSE106087 chip was downloaded from GEO to screen differential target genes; quercetin predicted target genes were collected from TCMSP, and then the intersection of differential genes with psoriasis NE were gained, target genes of quercetin intervention in psoriasis NE were collected and validated in String platform, meanwhile to perform GO and KEGG enrichment analysis, consequently, the mechanism of quercetin intervention in psoriasis NE was supported and analyzed via molecular docking evidence. Results1) There were 49454 genes contained in the GSE106087 gene chips, and 189 differential genes of psoriasis genes (36 genes for up regulation, and 153 for down regulation); GO enrichment analysis showed that there were 432 biological processes, 23 molecular components and six molecular function; KEGG enrichment analysis covers 12 signaling pathway; 2)There were 11 differential genes about quercetin intervention in psoriasis NE (seven genes for up regulation and four ones for down regulation), topology network filtering displayed that the core target genes were JUN and SSP1 (up-regulated genes for psoriasis NE), and there were eight genes through String platform validation, among them, six genes could perform molecular docking with quercetin, GO enrichment analysis showed 291 biological processes and seven molecular components and 14 molecular functions; and 34 signal pathways revealed in KEGG enrichment analysis. Conclusion1) GSE106087 gene chip contains 49454 genes. The pathological mechanism of psoriasis NE is closely related to the up-regulation of ARG1 and other genes, neutrophil activation, the interaction between virus protein and cytokines and cytokine receptors, and the down-regulation of SPP1 and other genes, neutrophil degranulation biological process and NOD-like receptor signaling pathway; 2)The mechanism of quercetin interfering with psoriasis NE is closely related to up-regulation of JUN and SSP1 and down-regulation of IL-17 signal pathway and TNF signal pathway.

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备注/Memo

备注/Memo:
陈广芳（1995—），女，硕士学位。研究方向：皮肤性病的中医诊治。国家重点研发计划“中医药现代化研究”重点专项课题（2018YFC1705301）；贵州省卫生健康委员会科学技术基金（2021XMSB00030852）。
更新日期/Last Update: 2023-09-15