[1]吴彬,韦国信,黄振鹏,等.不同时间窗丰富环境对阿尔茨海默病小鼠学习记忆能力变化的影响[J].西部中医药,2024,37(03):10-15.[doi:10.12174/j.issn.2096-9600.2024.03.03]
　WU Bin,WEI Guoxin,HUANG Zhenpeng,et al.Effects of the Environments Enriched by Different Time Windows on the Changes of Learning and Memory Ability in Alzheimer's Disease Mice[J].Western Journal of Traditional Chinese Medicine,2024,37(03):10-15.[doi:10.12174/j.issn.2096-9600.2024.03.03]
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不同时间窗丰富环境对阿尔茨海默病小鼠学习记忆能力变化的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年03期
页码:
10-15
栏目:
基础研究
出版日期:
2024-03-15

文章信息/Info

Title:
Effects of the Environments Enriched by Different Time Windows on the Changes of Learning and Memory Ability in Alzheimer's Disease Mice
作者:
吴彬, 韦国信, 黄振鹏, 黎剑云, 马秋平
广西中医药大学护理学院，广西 南宁 530200
Author(s):
WU Bin, WEI Guoxin, HUANG Zhenpeng, LI Jianyun, MA Qiuping
Nursing School, Guangxi University of Chinese Medicine, Nanning 530200, China
关键词:
阿尔茨海默病; 学习记忆能力; 丰富环境; 时间窗
Keywords:
Alzheimer's disease; learning and memory ability; enriched environment; time window
分类号:
R592
DOI:
10.12174/j.issn.2096-9600.2024.03.03
文献标志码:
A
摘要:
目的探讨不同时间窗丰富环境训练对阿尔茨海默病（Alzheimer's disease，AD）小鼠学习记忆能力的影响。 方法将30只人类淀粉样蛋白前体（amyloid precuesor protein，APP）/人类早老性痴呆基因1（presenilin 1，PS1）双转基因小鼠随机分为模型组、6月龄丰富环境（enriched environment，EE）组、9月龄EE组，C57BL/6小鼠10只为阴性对照组。6月龄EE组再分为EE6 h和EE12 h组，分别于6月龄每日丰富环境干预6 h和12 h。9月龄EE组再分为EE6 h和EE12 h组，于9月龄每日丰富环境干预6 h和12 h。持续干预14天后，进行Morris水迷宫实验检测小鼠空间学习记忆能力；苏木精-伊红染色（hematoxylin-eosin staining，HE）及尼氏染色观察小鼠海马体病理学形态及神经元数量。 结果6月龄和9月龄各组小鼠逃避潜伏期时间和组间效应差异均有统计学意义（P＜0.05）；同月龄EE12 h组小鼠进入目标象限次数多及穿越平台次数多于模型组，并在目标象限停留时间长于模型组（P＜0.05）；丰富环境各组HE染色、尼氏染色结果显示，细胞形态较完整，且集中分布。丰富环境各组小鼠海马体正常神经元数量多于模型组（P＜0.05）。 结论丰富环境可以改善AD小鼠学习记忆能力，减缓海马体神经元凋亡速度，长时间丰富环境干预效果明显，疾病较早期是进行干预的最佳起始时间点。
Abstract:
ObjectiveTo investigate the effects of enriched environment training through different time windows on the change of learning and memory ability in Alzheimer's disease (AD) mice. MethodsThirty APP/PS1 mice were randomly divided into the model group, the 6-month-old EE group, the 9-month-old EE group, and ten C57BL/6 mice were chosen as the negative control group. The 6-month-old EE group was separated into EE6 h and EE12 h groups, enriched with environmental intervention for 6h and 12 h per day, respectively, at six months of age. The 9-month-old EE group was allocated to EE6 h and EE12 h groups, enriched with environmental intervention for 6 h and 12 h daily at nine months of age. After 14 days, the Morris water maze experiment was conducted to detect spatial learning and memory ability, and HE staining and Nissen staining were used to observe the hippocampal pathology and neuron number. ResultsThe differences in escape latency time and between-group effects were statistically significant in 6-month-old and 9-month-old groups of mice (P<0.05). The 12 h EE group at the same month of age entered the target quadrant more often, crossed the platform more often and stayed in the target quadrant longer than the model group (P<0.05). The HE staining and Nissl staining cells in each group of EE have a relatively complete morphology and concentrated distribution. The number of normal neurons in the hippocampus was significantly higher in the EE group than in the model group (P<0.05). ConclusionEE can improve learning and memory ability and slow down hippocampal neuron apoptosis in AD mice, and the effect of prolonged enriched EE is obvious, early intervention of disease is the best start time.

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备注/Memo

备注/Memo:
吴彬（1963—），女，硕士研究生学历，教授。研究方向：护理教育及老年护理。广西中医药大学一流学科建设开放课题（2019XK063）；广西中医药大学高层次人才培育创新团队建设项目（2022A010）。
更新日期/Last Update: 2024-03-15