[1]郭敏,王婷婷,安培坤,等.当归配方颗粒及饮片的HPLC特征图谱研究及多成分含量分析[J].西部中医药,2024,37(12):1-6.[doi:10.12174/j.issn.2096-9600.2024.12.01]
　GUO Min,WANG Tingting,AN Peikun,et al.Study on HPLC Specific Chromatograms and Multi-component Content Analysis of Danggui Formula Granules and Decoction Pieces[J].Western Journal of Traditional Chinese Medicine,2024,37(12):1-6.[doi:10.12174/j.issn.2096-9600.2024.12.01]
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当归配方颗粒及饮片的HPLC特征图谱研究及多成分含量分析
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年12期
页码:
1-6
栏目:
质量分析
出版日期:
2024-12-15

文章信息/Info

Title:
Study on HPLC Specific Chromatograms and Multi-component Content Analysis of Danggui Formula Granules and Decoction Pieces
作者:
郭敏, 王婷婷, 安培坤, 罗燕燕, 王红丽
甘肃省中医院，甘肃 兰州 730050
Author(s):
GUO Min, WANG Tingting, AN Peikun, LUO Yanyan, WANG Hongli
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou730050, China
关键词:
当归; 饮片; 配方颗粒; 高效液相色谱; 相关性评价
Keywords:
decoction pieces; formula granules; HPLC; correlation evaluation
分类号:
R286.0
DOI:
10.12174/j.issn.2096-9600.2024.12.01
文献标志码:
A
摘要:
目的建立当归饮片及配方颗粒的高效液相色谱（high performance liquid chromatography，HPLC）特征图谱，进行相关性评价及4个成分含量分析。 方法应用“中药色谱指纹图谱相似度评价系统（2012版）”绘制18批样品指纹图谱，考察相似度；应用SPSS 22.0软件进行聚类分析，对当归药材及两个厂家（厂家A、厂家B）配方颗粒进行相关性评价及4个成分含量分析。 结果当归饮片及两个厂家配方颗粒HPLC特征图谱共有18个共有峰，相似度在0.698～1.000之间；指认出绿原酸、阿魏酸、洋川芎内酯Ⅰ、藁本内酯4个特征峰，线性方程分别为Y=8.5183X+40.389（R2=0.9995）、Y=19.951X+4.7477（R2=0.9994）、Y=52.466X+17.971（R2=0.9994）、Y=5.03X+6.68（R2=0.9993），平均加样回收率分别为99.60%、99.09%、98.13%、98.09%，重复性、精密度、稳定性试验RSD均小于3%；聚类分析结果显示当距离为2时，18批样品可聚为二类，其中S1～S6、S7～S18各聚为一类，与相似度评价结果一致；与饮片相比，配方颗粒中增加4号峰，18号峰差异大。 结论当归饮片与配方颗粒、配方颗粒之间HPLC图谱具有较高的相似度，但仍存在差异，为配方颗粒的质量控制及临床应用提供参考。
Abstract:
ObjectiveTo establish high performance liquid chromatography (HPLC) characteristic chroma-tograms of Danggui (Angelica sinensis) slices and its formula granules, and to conduct correlation evaluation and content analysis of the four components. MethodsThe fingerprints of 18 batches of samples were plotted using the "similarity evaluation system for chromatographic fingerprint of TCM (version 2012)" to investigate the similarity. Cluster analysis was performed with SPSS 22.0 software. The correlation evaluation and the content analysis of four components were performed on Danggui and the formula granules of two manufacturers (Manu-facturer A and Manufacturer B). ResultsThere are 18 common peaks in the HPLC characteristic chromatograms of Danggui decoction pieces and the formula granules of the two manufacturers, with similarities ranging from 0.698 to 1.000; four characteristic peaks of chlorogenic acid, ferulic acid, ligustilide I and ligustilide were identified, and the linear equations are Y=8.5183X+40.389 (R2=0.9995), Y=19.951X+4.7477 (R2=0.9994), Y=52.466X+17.971 (R2=0.9994), Y=5.03X+6.68 (R2=0.9993), the average sample recovery rates were 99.60%, 99.09%, 98.13%, and 98.09% respectively. RSDs of the repeatability, precision, and stability tests were all less than 3%; the cluster analysis results showed that when the distance was 2, 18 batches of the samples can be clustered into two categories, of which S1 to S6 and S7 to S18 are each clustered into one category, which is consistent with the similarity evaluation results. Compared with the decoction pieces, peak No. 4 is added to the formula particles, and peak No. 18 has a large difference. ConclusionThe HPLC fingerprints of Danggui decoction pieces and formula granules, between formula granules show high similarity, but there are still differences, which could provide a reference for the quality control and clinical application of formula granules.

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备注/Memo

备注/Memo:
郭敏（1983—），女，博士学位，副主任药师。研究方向：中药质量标准化及活性筛选。甘肃省中医药科研项目（GZK-2019-18）；甘肃省卫生行业专项（GSWSKY2018-46）。
更新日期/Last Update: 2024-12-15