[1]石晓冬,卢登勇,吴慧敏,等.基于网络药理学探讨黄芪-丹参干预肾纤维化的作用机制[J].西部中医药,2025,38(04):53-58.[doi:10.12174/j.issn.2096-9600.2025.04.11]
　SHI Xiaodong,LU Dengyong,WU Huimin,et al.Network Pharmacology-based Discussion on the Mechanism of Intervention of Renal Fibrosis with Huangqi-Danshen Couplet Medicines[J].Western Journal of Traditional Chinese Medicine,2025,38(04):53-58.[doi:10.12174/j.issn.2096-9600.2025.04.11]
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基于网络药理学探讨黄芪-丹参干预肾纤维化的作用机制()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年04期
页码:
53-58
栏目:
二次研究
出版日期:
2025-04-15

文章信息/Info

Title:
Network Pharmacology-based Discussion on the Mechanism of Intervention of Renal Fibrosis with Huangqi-Danshen Couplet Medicines
作者:
石晓冬1, 卢登勇1, 吴慧敏1, 陈宇珊1, 左金巾1, 钟建2
1.广西中医药大学研究生院，广西 南宁 530000
2.广西中医药大学第一附属医院，广西 南宁 530001
Author(s):
SHI Xiaodong1, LU Dengyong1, WU Huimin1, CHEN Yushan1, ZUO Jinjin1, ZHONG Jian2
1.Graduate School of Guangxi University of Chinese Medicine, Nanning 530000, China
2.The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530001, China
关键词:
肾纤维化; 网络药理学; 黄芪; 丹参
Keywords:
renal fibrosis; network pharmacology
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2025.04.11
文献标志码:
A
摘要:
目的探究黄芪-丹参药对防治肾纤维化的作用机制。 方法通过中药系统药理学数据库与分析平台（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）检索黄芪、丹参两味中药的活性成分和作用靶点；利用人类基因数据库（the human gene database，GeneCards）及在线人类孟德尔遗传数据库（online mendelian inheritance in man，OMIM）检索与肾纤维化相关的基因，并使用UniProt数据库校正靶点对应的基因名称；通过Cytoscape 3.6.0软件构建黄芪-丹参药对的活性成分-靶点网络及蛋白-蛋白互作（protein-protein interactions，PPI）网络，筛选出核心活性成分及靶点；使用核心靶基因导入基因功能注释数据库（the database for annotation visualization and integrated discovery，DAVID）进行基因本体论（gene ontology，GO）功能富集分析和京都基因和基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路富集分析。 结果得到黄芪-丹参干预肾纤维化的有效活性成分85个，有效作用靶点1297个；富集分析得出作用机制可能与PI3K/AKT、HIF-1、细胞凋亡、甲状腺激素、MAPK、钙、NF-κB、血管内皮生长因子等信号通路有关，且IL-6、CASP3、MAPK8、VEGFA、EGFR、MYC、CCND1、ESR1、FOS、ERBB2、AR、RELA、PPARG等可能是芪-丹参干预肾纤维化的重要靶点基因。 结论黄芪-丹参药对干预肾纤维化具有多成分-多靶点-多通路的特点。
Abstract:
ObjectiveTo survey the mechanism of Huangqi (Astragali radix)-Danshen (Salviae miltiorrhizae radix et rhizoma) in the prevention and treatment of renal fibrosis. MethodsActive ingredients and the targets of action of Huangqi and Danshen were searched from TCMSP; GeneCards and OMIM were utilized to search renal fibrosis-related genes, and UniProt database was used to correct the names of the genes corresponding to the targets; Cytoscape 3.6.0 software was applied to construct active ingredients-target network and PPI interaction of the couplet medicines, for the screening of the core active ingredients and targets; DAVID was used to perform GO and KEGG enrichment analysis. ResultsAll 85 effective active ingredients and 1297 effective targets of action were obtained from the couplet medicines; the enrichment analysis revealed that the mechanism might be associated to the signaling pathways including PI3K/AKT, HIF-1, cellular apoptosis, thyroid hormone, MAPK, calcium, NF-κB and vascular endothelial growth factors, and IL-6, CASP3, MAPK8, VEGFA, EGFR, MYC, CCND1, ESR1, FOS, ERBB2, AR, RELA and PPARG might be the important target genes for the intervention of renal fibrosis with the couplet medicines. Conclusion Huangqi-Danshen couplet medicines have the characteristics of multi-component, multi-target and multi-pathway for the intervention of renal fibrosis.

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备注/Memo

备注/Memo:
石晓冬（1997—），女，在读博士研究生。研究方向：中西医结合防治慢性肾脏病。国家自然科学基金地区基金项目（81760807）。
更新日期/Last Update: 2025-04-15