[1]黄赫,齐越,王若冰,等.济阴颗粒对雌性冠心病大鼠脂代谢的影响[J].西部中医药,2021,34(08):29-34.[doi:10.12174/j.issn.2096-9600.2021.08.07]
　HUANG He,QI Yue,WANG Ruobing,et al.Effects of Jiyin Granules on Lipid Metabolism in Female Rat with Coronary Heart Disease[J].Western Journal of Traditional Chinese Medicine,2021,34(08):29-34.[doi:10.12174/j.issn.2096-9600.2021.08.07]
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济阴颗粒对雌性冠心病大鼠脂代谢的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年08期
页码:
29-34
栏目:
出版日期:
2021-08-15

文章信息/Info

Title:
Effects of Jiyin Granules on Lipid Metabolism in Female Rat with Coronary Heart Disease
作者:
黄赫1, 齐越2, 王若冰1, 张瑜1, 赵磊1
1.辽宁中医药大学附属第二医院，辽宁 沈阳 110034
2.江苏省徐州医药高等职业学校
Author(s):
HUANG He1, QI Yue2, WANG Ruobing1, ZHANG Yu1, ZHAO Lei1
1.The Second Affiliated Hospital to Liaoning University of Traditional Chinese Medicine, Shenyang 110034, China
2.Jiangsu Provincial Xuzhou Pharmaceutical Vocational College
关键词:
冠心病; 脂代谢; CAV1; ABCA1; ApoA1; SRB1; LDLR; VLDLR; 雌性; 大鼠
Keywords:
CHD; lipid metabolism; CAV1; ABCA1; ApoA1; SRB1; LDLR; VLDLR; female; rat
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2021.08.07
摘要:
目的考察济阴颗粒对雌性冠心病大鼠脂代谢通路因子小窝蛋白1（caveolin-1，Cav-1）、低密度脂蛋白受体（low density lipoprotein receptor，LDLR）、极低密度脂蛋白受体（very low density lipoprotein receptor，VLDLR）、ATP结合盒转运蛋白家族转运蛋白A1（atp-binding cassette transporter?a1，ABCA1）、载脂蛋白A1（apolipoprotein-a1，ApoA1）、B1类清道夫受体（scavenger receptor class B type1，SRB1）mRNA水平、血清总胆固醇含量的影响。 方法健康雌性SD大鼠75只，除空白组15只外，其余60只制备绝经期冠心病模型，分为模型组、西药对照组、中药对照组、济阴颗粒组，每组15只。分离心脏主动脉，Elisa法测定各组大鼠血清总胆固醇含量，实时定量PCR方法检测雌性冠心病大鼠CAV1、LDLR、VLDLR、ABCA1、ApoA1、SRB1 mRNA水平。 结果实时定量PCR结果显示，同模型组比较，空白组、西药阳性组、中药阳性组、济阴颗粒组ABCA1、ApoA1、CAV1、LDLR、SRB1、VLDLR mRNA表达增加，差异有统计学意义（P＜0.05）。与空白组相比，济阴颗粒组ApoA1、CAV1、SRB1、VLDLR mRNA表达明显减少，差异有统计学意义（P＜0.01），济阴颗粒组ABCA1、LDLR mRNA表达差异无统计学意义。ELISA结果显示，与模型组比较，空白组、西药阳性组、中药阳性组总胆固醇含量显著减少，差异有统计学意义（P＜0.01），济阴颗粒组总胆固醇含量减少，具有统计学意义（P＜0.05）。 结论济阴颗粒可能通过降低去卵巢雌性冠心病大鼠总胆固醇含量，提高ABCA1、ApoA1、CAV1、LDLR、SRB1、VLDLR mRNA相对表达量来改善雌性大鼠冠心病。
Abstract:
ObjectiveTo investigate the effects of Jiyin granules on the levels of lipid metabolism pathway factors including CAV1, LDLR, VLDLR, ABCA1, ApoA1, SR-B1mRNA and serum total cholesterol in female rat with CHD. MethodsAmong 75 healthy female SD tats,except 15 rats of the balnk group the remaining 60 tats were prepared in rat models with menopaused CHD and they were randomized into the the model group, Western medicine control group and herbal control group and Jiyin granules group, 15 rats in each group by separating cardiac aorta, ELISA method was used to measure the contents of total cholesterol in the serum of the rats in different groups, real time quantitative PCR to detect the levels of CAV1, LDLR, VLDLR, ABCA1, ApoA1 and SRB1mRNA in female CHD rats. ResultsThe results of real time quantitative PCR showed, compared with the model group, the expressions of ABCA1, ApoA1, CAV1, LDLR, SRB1 and VLDLR mRNA of the blank group, Western medicine control group, herbal control group and Jiyin granules group increased, and the difference had statistical meaning (P<0.05). Compared with the blank group, the expressions of ApoA1, CAV1, SRB1 and VLDLR mRNA of Jiyin granules group reduced, and the difference had statistical meaning (P<0.01), the difference had no statistical meaning in the expressions of ABCA1 and LDLR mRNA of Jiyin granules group. ELISA results showed that, compared with the blank group, the contents of TC in the blank group, Western medicine control group and herbal control group lowered significantly, and the difference was statistically significant (P<0.01), the contents of TC in Jiyin granules group decreased, and it had statistical meaning (P<0.05). ConclusionJiyin granules could improve female CHD rat, which might be through lowering the contents of TC, raising the relative expressions of ABCA1, ApoA1, CAV1, LDLR, SRB1 and VLDLR mRNA in female CHD rats.

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备注/Memo

备注/Memo:
黄赫（1988—），女，硕士学位，助理研究员。研究方向：疾病的分子机理研究。
更新日期/Last Update: 2021-08-15