[1]赵军,李昕,刘晋,等.蒙药蓝刺头对去卵巢大鼠TGF-β1、BMD及子宫组织形态的影响[J].西部中医药,2021,34(10):30-33.[doi:10.12174/j.issn.2096-9600.2021.10.07]
　ZHAO Jun,LI Xin,LIU Jin,et al.Influence of Mongolian Medicine Lancitou on TGF-β1, BMD and Uterine Tissue Morphology of Ovariectomized Rats[J].Western Journal of Traditional Chinese Medicine,2021,34(10):30-33.[doi:10.12174/j.issn.2096-9600.2021.10.07]
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蒙药蓝刺头对去卵巢大鼠TGF-β1、BMD及子宫组织形态的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年10期
页码:
30-33
栏目:
出版日期:
2021-10-15

文章信息/Info

Title:
Influence of Mongolian Medicine Lancitou on TGF-β1, BMD and Uterine Tissue Morphology of Ovariectomized Rats
作者:
赵军1, 李昕2, 刘晋1, 王雄耀3, 师建平3, 董重阳3, 常虹2, 高小明2, 莫日根1, 谢静华1
1.内蒙古自治区中医医院，内蒙古 呼和浩特 010020
2.内蒙古医科大学附属医院
3.内蒙古医科大学中医学院
Author(s):
ZHAO Jun1, LI Xin2, LIU Jin1, WANG Xiongyao3, SHI Jianping3, DONG Chongyang3, CHANG Hong2, GAO Xiaoming2, MO Rigen1, XIE Jinghua1
1.Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine, Hohehot 010020, China
2.Affiliated Hospital of Inner Mongolia Medical University
3.TCM College, Inner Mongolia Medical University
关键词:
骨质疏松; 绝经后; 蒙药蓝刺头; 骨密度; 转化生长因子; 子宫; 雌激素经典通路; 动物实验
Keywords:
osteoporosis;  postmenopausal; mongolian medicine; bone mineral density; TGF-; uterus; estrogen classical signaling pathway; animal experiments
分类号:
R274.9
DOI:
10.12174/j.issn.2096-9600.2021.10.07
摘要:
目的研究蒙药蓝刺头对绝经后骨质疏松症（postmenopausal osteoporosis，PMOP）的防治作用，阐述其在蒙医“补暖补精，固骨质壮骨”理论下促进骨形成、抑制破骨的机制。 方法取Wistar大鼠77只，随机分为蓝刺头高、中、低剂量组，强骨胶囊组，己烯雌酚组，模型组，假手术组，每组11只。通过去卵巢复制PMOP模型，用蒙药蓝刺头、强骨胶囊和己烯雌酚分别灌胃模型大鼠90天，观察比较各组大鼠血清转化生长因子β1（transforming growth factor-β1，TGF-β1）、骨密度（bone mineral density，BMD）、子宫病理学变化情况。 结果与模型组比较，中剂量的蒙药蓝刺头能有效防治绝经后骨质疏松症模型大鼠骨质疏松症，同时明显提高绝经后骨质疏松症模型大鼠血清TGF-β1水平（P＜0.05）。子宫HE染色切片分析显示：中剂量的蒙药蓝刺头能显著改善大鼠去卵巢后对于子宫的影响，子宫肌全层明显增厚、内膜腺体内部的直径明显增大、内膜腺体腔内面积所占视野百分比明显增大、内膜上皮深度明显增大（P＜0.05）。 结论蒙药蓝刺头具有提高去势大鼠BMD作用，一定程度上抑制了雌激素缺乏导致的子宫功能的丧失，其通过雌激素经典通路对于PMOP有防治作用。
Abstract:
ObjectiveTo illustrate the mechanism of promoting bone regeneration and inhibiting bone destroying guided by the calssical theory of mongolian medicine by studying the effects of mongolian medicine Lancitou on postmenopausal osteoporosis (PMOP) via estrogen classical signaling pathway. MethodsAll 77 Wistar rats were chosen and randomized into high, moderate and low doses groups of Lancitou, Qianggu capsules group, diethylstilbestrol (DES) group, the model group and sham operation group, 11 rats each group. PMOP models were established by ovariectomizing; rat models were drenched with mongolian medicine Lancitou, Qianggu capsules and DES for 90 days, to observe and compare serum transforming growth factor β1(TGF- β1), bone mineral density (BMD) and uterine pathology of the rats in different groups. ResultsCompared with the model group, moderate dose of mongolian medicine Lancitou could effectively prevent osteoporosis of PMOP rats from BMD, and simultaneously lift TGF-β1 levels obviously (P<0.05). The analysis of uterine slice after HE staining showed: moderate dose of mongolian medicine Lancitou could remarkably improve ovariectomizing influence on the uterus, total uterine muscle thickened notably, internal diameter of endometrial glands enlarged, visual field percentage of endometrial glands cavity area and endometrial epithelial depth increased notably (P<0.05). ConclusionMongolian medicine Lancitou could raise BMD of ovariectomized rats, inhibit the loss of uterine function caused by estrogen to a certain extent, and it could effectively prevent and treat PMOP through estrogen classical signaling pathway.

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备注/Memo

备注/Memo:
赵军（1988—），男，硕士学位，主治医师。研究方向：中医骨伤科疾病的研究。国家自然科学基金（30560165）；内蒙古自治区自然科学基金（2009MS1134）；国家中医药管理局中医药传承与创新“百千万人才工程”项目（J20184832009）；内蒙古自治区第三批老蒙医药中医药专家学术经验继承项目（内卫计蒙中字［2019］126）；内蒙古自治区草原英才创新团队“中医治未病”项目（CYYCTD2018）；内蒙古医科大学“科技百万工程”联合项目［YKD2017KJBW（LH）064］；第四批全国中医（临床、基础）优秀人才研修项目（J20184832009）。
更新日期/Last Update: 2021-10-15