[1]段桂姣,王振常,吴珊珊.柔肝化纤颗粒对肝纤维化模型大鼠肝组织超氧化物歧化酶和丙二醛的影响[J].西部中医药,2022,35(04):30-34.[doi:10.12174/j.issn.2096-9600.2022.04.08]
 DUAN Guijiao,WANG Zhenchang,Wu Shanshan.Effects of Rougan Huaxian Granules on SOD and MDA in Liver Tissue of Rats with Hepatic Fibrosis[J].Western Journal of Traditional Chinese Medicine,2022,35(04):30-34.[doi:10.12174/j.issn.2096-9600.2022.04.08]
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柔肝化纤颗粒对肝纤维化模型大鼠肝组织超氧化物歧化酶和丙二醛的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年04期
页码:
30-34
栏目:
药理研究
出版日期:
2022-04-15

文章信息/Info

Title:
Effects of Rougan Huaxian Granules on SOD and MDA in Liver Tissue of Rats with Hepatic Fibrosis
作者:
段桂姣1 王振常2 吴珊珊1
1.广西玉林第一人民医院,广西 玉林 537000
2.广西国际壮医医院
Author(s):
DUAN Guijiao1 WANG Zhenchang2 Wu Shanshan1
1.Yulin City First People’s Hospital, Yulin 537000, China
2.Guangxi International Zhuang Medicine Hospital
关键词:
肝纤维化柔肝化纤颗粒超氧化物歧化酶丙二醛氧化应激
Keywords:
hepatic fibrosisgranulesSODMDAoxidative stress
分类号:
R256.4
DOI:
10.12174/j.issn.2096-9600.2022.04.08
文献标志码:
A
摘要:
目的观察柔肝化纤颗粒对四氯化碳诱导的肝纤维化模型大鼠肝组织中超氧化物歧化酶(superoxide dismutase,SOD)和丙二醛(malondialdehyde,MDA)的影响。 方法选择50只SD大鼠,其中空白组12只,其余38只制备四氯化碳(CCl4)诱导的肝纤维化大鼠模型,造模后随机分为模型组、柔肝化纤组及秋水仙碱组,每组12只。各组给予对应药物灌胃,连续给药4周。检测大鼠血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate transaminase,AST)水平,计算肝脏脏器指数。放射免疫法检测血清透明质酸(hyaluronic acid,HA)、层黏蛋白(laminin,LN)、Ⅲ型前胶原(Collagen Ⅲ protein,P3NP)、Ⅳ型胶原(Collagen IV,CⅣ)的水平。检测肝组织中SOD和MDA水平。HE染色法观察肝组织纤维化程度。 结果与模型组比较,柔肝化纤组大鼠血清中ALT、AST、HA、LN、P3NP、CⅣ水平显著降低(P<0.05),肝脏组织中SOD水平升高(P<0.05),MDA水平降低(P<0.05),肝脏指数降低(P<0.05)。柔肝化纤组大鼠肝组织结构明显改善,肝细胞脂肪变性减轻,少许炎性细胞浸润与假小叶形成。 结论柔肝化纤颗粒可以降低肝纤维化大鼠肝组织中SOD和MDA含量,通过发挥其抗氧化作用减轻CCl4诱导的肝纤维化程度。
Abstract:
ObjectiveTo observe the effects of Rougan Huaxian granules on SOD and MDA in liver tissue of rats with hepatic fibrosis induced by carbon tetrachloride (CCI4). MethodsFifty SD rats were chosen, among them, 12 ones were selected into the blank group, the remaining 38 rats were prepared into rat models with hepatic fibrosis induced by CCI4, after modeling, they were randomized into the model group, Rougan Huaxian granules group and colchicine group, with 12 ones in each group. Different groups were drenched with the corresponding medicine, administration for four consecutive weeks. To detect the levels of AST and ALT in rat serum, and to calculate liver organ index. To measure the levels of HA, LN, P3NP and CⅣ by radioimmunoassay (RIA). To measure the levels of SOD and MDA in liver tissue. To observe the degrees of hepatic fibrosis in liver tissue by HE staining method. ResultsCompared with the model group, the levels of ALT, AST, HA, LN, P3NP and CⅣ lowered significantly in Rougan Huaxian granules group (P<0.05), the levels of SOD raised in liver tissue (P<0.05), the levels of MDA decreased (P<0.05), liver index reduced (P<0.05). Structural improvements of liver tissue in Rougan Huaxian granules group were notable, hepatocyte steatosis reduced, a few inflammatory cells infiltrated and pseudolobule formed. ConclusionRougan Huaxian granules could cut down the contents of SOD and MDA, and relieve the degrees of CCI4-induced hepatic fibrosis by developing its antioxidation effects.

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备注/Memo

备注/Memo:
段桂姣(1992—),女,硕士学位,住院医师。研究方向:肝纤维化、肝硬化的中西医结合基础与临床研究。国家自然科学基金(81660745,81960910)。
更新日期/Last Update: 2022-06-24