[1]马毅,晋玲△,王振恒,等.HPLC测定不同产地麻黄中麻黄碱和伪麻黄碱的含量[J].西部中医药,2012,25(07):14-16.
　MA Yi,JIN Ling,WANG Zhen-heng,et al.Determination of Ephedrine and D-pseudephedrine in MaHuang from Different Places with HPLC[J].Western Journal of Traditional Chinese Medicine,2012,25(07):14-16.
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HPLC测定不同产地麻黄中麻黄碱和伪麻黄碱的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年07期
页码:
14-16
栏目:
出版日期:
2012-07-15

文章信息/Info

Title:
Determination of Ephedrine and D-pseudephedrine in MaHuang from Different Places with HPLC
文章编号:
1004-6852(2012)07-0014-03
作者:
马毅，晋玲△，王振恒，崔治家，李应东
甘肃中医学院药学系，甘肃 兰州 730000
Author(s):
MA Yi, JIN Ling, WANG Zhen-heng, CUI Zhi-jia, LI Ying-dong
Pharmacy Department in Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
关键词:
麻黄; 高效液相色谱; 麻黄碱; 伪麻黄碱
Keywords:
MaHuang;  HPLC;  ephedrine;  D-pseudephedrine
分类号:
R284.1
文献标志码:
A
摘要:
目的：运用高效液相色谱法，对不同地区中麻黄生物碱(包括盐酸麻黄碱和盐酸伪麻黄碱)含量进行比较分析。方法：采用高效液相色谱法，色谱柱为C18(250 mm×4.6 mm，50 μm］，流动相为V［乙腈］：V［0.02%磷酸水溶液］=4：96，流速1.0 mL/min，柱温30℃，检测波长210 nm。结果：四产地样品麻黄碱含量和伪麻黄碱含量有显著性差异(P＜0.05)，麻黄碱含量新疆＞青海＞甘肃＞山西，伪麻黄碱含量新疆＞甘肃＞青海＞山西，但其总含量无显著性差异，均符合药典总碱含量不少于0.8%的用药标准。结论：通过对不同产地麻黄进行生物碱含量分析，为麻黄药材的利用提供依据，有助于麻黄药材的标准化种植及质量控制。
Abstract:
Objective: To make comparative analysis on contents of ephedra alkaloid(including ephedrine and D-pseudephedrine) with HPLC. Method: HPLC was used, chromatographic column was C18(250 mm×4.6 mm, 50 μm), the mobile phase was V(methyl cyanide):V(0.2% ammonium dihydrogen phosphate solution)=4:96, flow rates was 1.0 mL/min, the temperature was 30℃, length of detection wave was 210nm. Result: The contents of ephedrine(P＜0.05) and D-pseudephedrine in four samples from different habitats showed notable differences(P＜0.05), contents of ephedrine ranked Xinjiang＞Qinghai＞Gansu＞Shanxi, contents of D-pseudephedrine ranked Xinjiang＞Gansu＞Qinghai＞Shanxi, but comparison of total contents suggested no remarkable difference and all samples met the standard that content of total alkalis was not less than 0.8% in Pharmacopeia. Conclusion: Analysis on contents of alkaloid in MaHuang from different habitats could provide evidence for the utilization of MaHuang(Ephedrae), helpful for standardized plant and quality control of MaHuang.

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备注/Memo

备注/Memo:
收稿日期：2010-03-07 *基金项目：科技部十二五科技支撑项目(编号2011BAI05B02) 作者简介：马毅(1972—)，男，硕士学位，讲师。研究方向：药用植物资源开发利用及生物多样性研究。 △通讯作者：晋玲(1974—)，女，博士学位，硕士研究生导师，副教授。研究方向：药用植物资源及种质研究。
更新日期/Last Update: 2012-07-15