[1]李妍怡,杨瑞龙,刘志军,等.补脑膏对脑缺血再灌注损伤模型大鼠神经保护作用的机制研究[J].西部中医药,2013,26(10):14-16.
　LI Yanyi,YANG Ruilong,LIU Zhijun,et al.Study on the Mechanism of Neuroprotective Function of BuNaoGao for Rat Model with Cerebral Ischemia Reperfusion Injury[J].Western Journal of Traditional Chinese Medicine,2013,26(10):14-16.
点击复制

补脑膏对脑缺血再灌注损伤模型大鼠神经保护作用的机制研究
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年10期
页码:
14-16
栏目:
出版日期:
2013-10-15

文章信息/Info

Title:
Study on the Mechanism of Neuroprotective Function of BuNaoGao for Rat Model with Cerebral Ischemia Reperfusion Injury
文章编号:
1004-6852(2013)10-0014-03
作者:
李妍怡1，杨瑞龙1，刘志军1，张小荣1，东 红1，朱若昕2
1 甘肃省中医院，甘肃 兰州 730050； 2 沈阳医学院
Author(s):
LI Yanyi1, YANG Ruilong1, LIU Zhijun1, ZHANG Xiaorong1, DONG Hong1, ZHU Ruoxin2
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Shenyang Medical College
关键词:
补脑膏; 脑缺血再灌注损伤; NGF; BDNF
Keywords:
BuNaoGao;  cerebral ischemia reperfusion injury;  NGF;  BDNF
分类号:
R255.2
文献标志码:
A
摘要:
目的：观察补脑膏对大鼠局灶性脑缺血再灌注损伤后的神经保护作用，并探讨其作用机制，为补脑膏临床治疗中风提供理论依据和技术支撑。方法：雄性SPF级SD大鼠80只，随机分为假手术组8只、模型组24只、补脑膏大剂量组24只、补脑膏小剂量24只。其中模型组、补脑膏大剂量组、补脑膏小剂量组再分为缺血再灌注24、48和72小时3个亚组，各亚组8只大鼠。除假手术组外，均采用大脑中动脉线栓法制备大鼠脑缺血再灌注损伤模型，各药物组再灌1小时后，灌胃给药补脑膏，2次/d；模型组予生理盐水灌胃。模型组及各药物组于再灌注3小时观察神经功能缺损症状并进行评分，再灌注24、48和72小时末断头取材，TTC染色检测脑梗死组织比重、ELISA法测定脑组织中NGF、BDNF表达量。结果：①补脑膏大剂量组在再灌注48、72小时与模型组比较，大鼠神经功能缺损评分具有显著差异(P＜0.05)；而补脑膏小剂量组仅在再灌注72小时，大鼠神经功能缺损评分方面与模型组比较存在差异(P＜0.05)。②补脑膏大、小剂量组与模型组相比，梗死脑组织比重明显降低(P＜0.05)。③再灌注24、48、72小时时，补脑膏大、小剂量组脑组织脑源性神经营养因子(BDNF)、神经生长因子(NGF)的表达较模型组均增加(P＜0.05)；而补脑膏大剂量组NGF的表达高于小剂量组(P＜0.05)，2组间BDNF的表达无显著差异。结论：补脑膏可通过减轻脑组织梗死比重，上调NGF、BDNF的表达，通过缓解脑缺血再灌注损伤神经缺损症状，而起到脑神经保护作用。
Abstract:
Objective: To observe neuroprotective function of BuNaoGao for the rat suffering from focal cerebral ischemia reperfusion injury, to discuss its mechanism, therefore to provide theoretical evidence and technical support for the therapy. Method: All 80 rats were randomized into 8 rats in the sham operation group, 24 rats in the model group, 24 rats in high dosage group of BuNaoGao and 24 rats in the low dosage group of BuNaoGao. Among them, the model group, high dosage group and low dose group of BuNaoGao could be divided into three sub-groups at 24, 48 and 72 hours of ischemia-reperfusion, 8 rats each subgroup. Except sham operation group, the model was established by middle cerebral artery occlusion(MCAO), after reperfusion for one hour, the models received intragastric administration of BuNaoGao, twice per day; the model group received lavage of physiological saline. After reperfusion for three hours, neurological impairment symptoms were observed and assessed, after reperfusion for 24, 48 and 72 hours, the models were executed and the sampling was took, the proportion of cerebral infarction tissue was detected by TTC dying, the expressions of NGF and BDNF in cerebral tissue were tested with ELISA method. Result: ① The difference was very significant in neurological impairment scale of rat when high dosage group of BuNaoGao after reperfusion for 24 hours and 72 hours was compared with the model group(P＜0.05); there was difference when the low dosage group of BuNaoGao was compared with the model group in neurological impairment scale of rat(P＜0.05). ② The proportion was decreased significantly when high dosage group and low dosage group of BuNaoGao were compared with the model group(P＜0.05). ③ After reperfusion for 24 hours, 48 hours and 72 hours, the expressions of high dosage group and low dosage group of BuNaoGao were higher than that of the model group(P＜0.05); While the expression of NGF in high dosage group of BuNaoGao was higher than that of the low dosage group of BuNaoGao(P＜0.05), there was no significant difference in the expression of BDNF between both groups. Conclusion: BuNaoGao could improve the expressions of NGF and BDNF through relieving the proportion of cerebral tissue infarction, and play neuroprotective function through alleviating neurological impairment symptom induced by cerebral ischemia-reperfusion.

相似文献/References:

[1]杨 涛,杨瑞龙.中西医结合治疗脑小血管病伴发非痴呆血管性认知功能损害的临床研究[J].西部中医药,2015,28(04):111.
[2]李伟艺,刘红松,高山瑛,等.基于Notch1/NF-κB信号通路探究补阳还五汤对脑缺血再灌注损伤的拮抗作用及其机制[J].西部中医药,2022,35(11):16.[doi:10.12174/j.issn.2096-9600.2022.11.04]
　LI Weiyi,LIU Hongsong,GAO Shanying,et al.Protective Effects of Buyang Huanwu Tang on Cerebral Ischemia-reperfusion Injury Based on Notch1/NF-κB Signaling Pathway and Its Mechanism[J].Western Journal of Traditional Chinese Medicine,2022,35(10):16.[doi:10.12174/j.issn.2096-9600.2022.11.04]
[3]李妍怡,东红,杨瑞龙,等.补脑膏对脑缺血再灌注损伤模型大鼠脑组织VEGF含量的影响[J].西部中医药,2014,27(10):16.

备注/Memo

备注/Memo:
收稿日期：2013-02-26 作者简介：李妍怡(1950—)，女，主任医师，硕士研究生导师，甘肃省名中医，全国中西医结合学会神经科学会委员，甘肃省中西医结合学会神经科专业委员会主任委员。研究方向：心脑血管疾病的中医药防治。
更新日期/Last Update: 2013-10-15