[1]张翌蕾,潘文△,汪永娟,等.补肾化瘀方对PCOS大鼠血清激素水平的影响[J].西部中医药,2016,29(02):5-8.
　ZHANG Yilei,PAN Wen,WANG Yongjuan,et al.The Influence of BuShen HuaYu Prescription on the Levels of Hormone of PCOS Rats[J].Western Journal of Traditional Chinese Medicine,2016,29(02):5-8.
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补肾化瘀方对PCOS大鼠血清激素水平的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年02期
页码:
5-8
栏目:
出版日期:
2016-02-15

文章信息/Info

Title:
The Influence of BuShen HuaYu Prescription on the Levels of Hormone of PCOS Rats
文章编号:
1004-6852(2016)02-0005-04
作者:
张翌蕾1，潘文2△，汪永娟1，王贵霞3，李明明1
1 甘肃中医药大学，甘肃 兰州 730000；2 甘肃省中医药研究院；3 甘肃省中医院
Author(s):
ZHANG Yilei1, PAN Wen2△, WANG Yongjuan1, WANG Guixia3, LI Mingming1
1 Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;2 Gansu Provincial Academy of Chinese Medicine; 3 Gansu Provincial Hospital of Traditional Chinese Medicine
关键词:
多囊卵巢综合征; 补肾化瘀方; 胰岛素抵抗; 激素水平
Keywords:
polycystic ovarian syndrome;  BuShen HuaYu prescription;  insulin;  endocrine levels
分类号:
R285.5
文献标志码:
A
摘要:
目的：观察补肾化瘀方对多囊卵巢综合征(PCOS)大鼠内分泌水平的影响。方法：选取120只6周龄雌性SD大鼠，设立空白对照组20只，余100只大鼠采用来曲唑溶液对大鼠灌胃，并喂以高脂高糖饲料建立PCOS大鼠模型，成模大鼠随机分为5组：模型对照组、二甲双胍组、中药低浓度组、中药中浓度组、中药高浓度组，每组20只。分别给予相应药物进行干预，干预14天后采用酶联免疫（ELISA）法测定大鼠血清雌醇（E1）、双氢睾酮（DHT）、促性腺激素释放激素（GnRH）、胰岛素（INS）水平，HE染色观察各组大鼠卵巢形态学变化。结果：与空白对照组比较，模型对照组血清E1、DHT、INS水平明显增高(P＜0.05)，卵泡呈囊性扩张，颗粒细胞层数减少，卵泡个数增多，卵母细胞消失；与模型对照组比较，各治疗组大鼠血清E1、DHT、INS水平显著降低(P＜0.05)，卵巢HE染色镜下可见黄体以及不同阶段卵泡、颗粒细胞层数明显增多，卵泡内可见卵母细胞以及放射冠。结论：补肾化瘀方可有效改善PCOS大鼠血清激素水平，降低INS水平，改善E1过高引起的高雌酮血症，并且能够改善卵巢功能与排卵情况。
Abstract:
Objective: To observe the influence of BuShen HuaYu prescription on endocrine levels of the rats with polycystic ovarian syndrome (PCOS). Methods: All 120 rats were chosen, 20 rats were established as blank control group, the rest 100 rats received intragastric administration of letrozole solution, and they were fed with high fat and high sugar fodder to establish PCOS rat model, the models were randomized into five groups: model control group, metformin group, high, moderate and low doses groups of herbs, 20 rats each group. The rats were intervened by the corresponding medicine, the levels of estrogen(E1), dihydrotestosterone(DHT), gonadotropin-releasing hormone(GnRH) and insulin(INS) were detected by adopting ELISA method, and morphological changes of ovaries were observed by HE staining method after intervened for 14 days. Results: Compared with blank control group, the levels of E1, DHT and INS of model control group increased obviously(P＜0.05), ovarian follicles were in cystic dilatation, granular cell layers decreased, the number of ovarian follicles increased, oocytes disappeared; compared with model control group, the levels of E1, DHT and INS lowered in different groups significantly (P＜0.05). Corpora luteum and ovarian follicles in different stages could be seen, granular cell layers increased notably, oocytes and corona radiate could be seen in the ovarian follicles under HE chromoendoscopy. Conclusion: BuShen HuaYu prescription could effectively improve endocrine levels of PCOS rats, decrease the levels of INS and improve high E1-induced ketonemia, ovarian function and ovulation.

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备注/Memo

备注/Memo:
*基金项目：国家自然科学基金资助项目（编号81360604）；甘肃省技术研究与开发专项计划项目（编号1305TCYA029）；甘肃省教育厅硕士研究生导师项目（编号1206-07)；甘肃省卫生行业科研计划管理项目（编号GWGL2013-3）。作者简介：张翌蕾（1990—），女，硕士研究生。研究方向：中医妇科血瘀证。△通讯作者：潘文(1963—)，女，硕士研究生导师，主任医师，甘肃省领军人才，甘肃省 555 创新人才，甘肃省卫生厅中青年学术技术带头人，中华中医药学会理事，中华中医药学会编辑出版分会副主任委员，中华中医药学会科普分会副主任委员，中华中医药学会文化分会常务理事，甘肃省中医药学会副会长兼副秘书长，甘肃省中医药学会文化专业委员会副主任委员兼秘书长，甘肃省中西医结合学会妇科专业委员会主任委员。研究方向：补肾活血法治疗多囊卵巢综合征伴胰岛素抵抗的机理研究。
更新日期/Last Update: 2016-02-15