[1]鲁利娜,王晶娟△,王新利,等.当归质量控制成分遴选研究[J].西部中医药,2017,30(10):22-26.
　LU Lina,WANG Jingjuan,WANG Xinli,et al.Study on Selection of Quality Control Components in DangGui[J].Western Journal of Traditional Chinese Medicine,2017,30(10):22-26.
点击复制

当归质量控制成分遴选研究()
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年10期
页码:
22-26
栏目:
出版日期:
2017-10-15

文章信息/Info

Title:
Study on Selection of Quality Control Components in DangGui
文章编号:
1004-6852(2017)10-0022-05
作者:
鲁利娜1，王晶娟1△，王新利2，刘 洋1，荣立新3，郭怡祯1，刘傲雪1
1 北京中医药大学，北京 100102； 2 北京春风药业有限公司； 3 中国中医科学院广安门医院
Author(s):
LU Lina1, WANG Jingjuan1△, WANG Xinli2, LIU Yang1, RONG Lixin3, GUO Yizhen1, LIU Aoxue1
1 Beijing University of Chinese Medicine, Beijing 100102, China; 2 Beijing Chunfeng Pharmaceutical Limited Company; 3 Guanganmen Hospital of Chinese Academy of Traditional Chinese Medicine
关键词:
当归; 水煎液; 肠吸收; 入血成分; 质控成分
Keywords:
DangGui;  decoction;  intestinal absorption;  blood intake component;  quality control component
分类号:
R932
文献标志码:
A
摘要:
目的：研究当归水煎液化学成分体内代谢情况，结合肠吸收遴选当归质量控制成分。方法：采用翻转肠囊法与腹主动脉采血试验，结合高效液相色谱法(HPLC)指纹图谱，研究当归水煎液化学成分的肠吸收与入血情况。结果：通过翻转肠囊实验，明确了22种通过肠吸收的成分，其中成分1、3、4、5、6、7、11、12、13、14、15、16(阿魏酸)、17、18(洋川芎内酯I)、19(洋川芎内酯H)、20、21、22以原型成分被肠吸收，成分M1、M2、M3、M4是通过肠壁酶代谢的新成分。分析腹主动脉采血HPLC明确了5种成分化合物12、18(洋川芎内酯I)、19(洋川芎内酯H)、M5、M6入血，其中成分12、18(洋川芎内酯I)、19(洋川芎内酯H)可直接通过肠吸收入血，成分M5、M6为经过代谢吸收入血的新成分。结论：综合肠吸收实验与入血成分分析，初步遴选出成分12、18(洋川芎内酯I)、19(洋川芎内酯H)作为当归的质量控制成分。
Abstract:
Objective: To study the metabolism in vivo of chemical composition in the decoction of DangGui (Angelica sinensis) and select the quality control component of DangGui by combining with intestinal absorption. Methods: The everted gut sac method and abdominal aorta blood sampling test as well as the combination of HPLC fingerprinting were adopted to investigate the absorption and blood supply of chemical constituents in the decoction of DangGui. Results: Twenty-two intestinal absorption components were identified by everted gut sac tests, and among which, components 1, 3, 4, 5, 6, 7, 11, 12, 13, 14, 15 and 16 (ferulaic acid), 17, 18 (Senkyunolide I), 19 (Senkyunolide H), 20, 21 and 22 were absorbed by intestine with prototype components. At the same time, M1, M2, M3 and M4 we are the new components with enzymatic metabolism through the intestinal wall. It was identified by the analysis of abdominal aortic blood HPLC that the five compounds 12, 18 (senkyunolide I), 19 (senkyunolide H), M5, M6 were in blood. Among which, the composition of 12, 18 (senkyunolide I) and 19 (senkyunolide H) were directly absorbed into the blood through intestine while M5 and M6 were the new components absorbed into the blood by metabolism. Conclusion: Components 12, 18 (senkyunolide I) and 19 (senkyunolide H) are selected preliminarily as the quality control components of DangGui by the analysis of intestinal absorption experiment and blood intake components.

相似文献/References:

[1]万娜,李朝平.当归对先兆流产大鼠模型流产率及血清β-HCG、血浆TXB_2含量的影响[J].西部中医药,2011,24(11):11.
[2]杨志军,李初谊,郭胜△,等.当归及不同配伍对环磷酰胺诱导的小鼠免疫器官、吞噬功能的影响[J].西部中医药,2013,26(04):8.
　YANG Zhijun,LI Chuyi,GUO Sheng,et al.Influence of DangGui and Its Compatibility on Immune Organs and Phagocytic Function of Mice Induced by Cyclophosphamide[J].Western Journal of Traditional Chinese Medicine,2013,26(10):8.
[3]肖吉元,杨扶德△,罗文蓉,等.当归与欧当归的鉴定学比较研究[J].西部中医药,2012,25(08):32.
　XIAO Ji-yuan,YANG Fu-de,LUO Wen-rong,et al.Comparative Study on Identification of DangGui and OuDangGui[J].Western Journal of Traditional Chinese Medicine,2012,25(10):32.
[4]李冬华,马潇,宋平顺,等.三种方法提取当归挥发性成分的气相色谱-质谱联用分析[J].西部中医药,2013,26(08):15.
　LI Donghua,MA Xiao,SONG Pingshun,et al.Analysis of Three Methods for Extracting Volatile Constituent from DangGui by Combination of Gas Chromatography and Mass Spectrometry[J].Western Journal of Traditional Chinese Medicine,2013,26(10):15.
[5]张艳萍,张明,任鹏飞,等.甘肃不同产地当归对小鼠心肌功能及组织形态影响的比较研究*[J].西部中医药,2014,27(02):14.
　ZHANG Yanping,ZHANG Ming,REN Pengfei,et al.Comparative Study on the Effects of DangGui from Different Habitats of Gansu on Myocardial Function and Tissue Morphology of Mice[J].Western Journal of Traditional Chinese Medicine,2014,27(10):14.
[6]任鹏飞,邓毅△.当归及其有效成分药效学研究进展[J].西部中医药,2012,25(09):125.
　REN Peng-fei,DENG Yi.Study Progress on Pharmacodynamics of DangGui and Its Active Ingredients[J].Western Journal of Traditional Chinese Medicine,2012,25(10):125.
[7]陈有智,罗文蓉,侯 嘉,等.不同产地当归的鉴定学研究[J].西部中医药,2012,25(05):25.
　CHEN You-zhi,LUO Wen-rong,HOU Jia,et al.Study on Identification of DangGui from Different Habitats[J].Western Journal of Traditional Chinese Medicine,2012,25(10):25.
[8]李镇航,夏鹏飞,李宁,等.道地产区当归药材数学判别模式的构建研究*[J].西部中医药,2014,27(02):4.
　LI Zhenhang,XIA Pengfei,LI Ning,et al.Analysis on Mathematical Discriminatory Model of Genuine DangGui from Famous Region[J].Western Journal of Traditional Chinese Medicine,2014,27(10):4.
[9]金国泰,李 博,王树荣△.柴胡解热的物质基础、药效及机制研究[J].西部中医药,2014,27(02):20.
　JIN Guotai,LI Bo,WANG Shurong.Research on the Mechanism, the Effects and Material Foundation of ChaiHu in Relieving Fever[J].Western Journal of Traditional Chinese Medicine,2014,27(10):20.
[10]马科,罗国平,宋紫霞,等.白头翁水煎液对离体蛙心功能的影响及其机制研究[J].西部中医药,2014,27(04):8.
　MA Ke,LUO Guoping,SONG Zixia,et al.Study on BaiTouWeng Decoction Influencing on Isolated Frog Heart and the Mechanism of the Therapy[J].Western Journal of Traditional Chinese Medicine,2014,27(10):8.

备注/Memo

备注/Memo:
收稿日期：2017-02-24 *基金项目：国家自然科学基金资助项目(编号81303217)。 作者简介：鲁利娜(1989—)，女，在读硕士研究生。研究方向：中药质量评价。 △通讯作者：王晶娟(1977—)，女，博士学位，副教授，硕士研究生导师。研究方向：中药质量评价与药效组分研究。
更新日期/Last Update: 2017-10-15