[1]牛媛瑕,张艳,魏丽琼,等.阿魏酸诱导胃癌SGC-7901细胞凋亡及对COX-2、Survivin、XIAP和p53的影响[J].西部中医药,2019,32(01):19-23.
　NIU Yuanxia,ZHANG Yan,WEI Liqiong,et al.The Inducing Effects of Ferulic Acid on the Apoptosis of Gastric Cancer SGC-7901 Cells and Its Influence on COX-2, Survivin, XIAP and p53[J].Western Journal of Traditional Chinese Medicine,2019,32(01):19-23.
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阿魏酸诱导胃癌SGC-7901细胞凋亡及对COX-2、Survivin、XIAP和p53的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年01期
页码:
19-23
栏目:
出版日期:
2019-01-15

文章信息/Info

Title:
The Inducing Effects of Ferulic Acid on the Apoptosis of Gastric Cancer SGC-7901 Cells and Its Influence on COX-2, Survivin, XIAP and p53
文章编号:
1004-6852(2019)19-0019-05
作者:
牛媛瑕1，张艳2，魏丽琼3，徐燕2△
1 甘肃中医药大学附属医院检验科，甘肃 兰州 730020； 2 甘肃中医药大学； 3 甘肃省妇幼保健院儿童保健科
Author(s):
NIU Yuanxia1, ZHANG Yan2, WEI Liqiong3, XU Yan2△
1 Clinical Lab, Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730020, China; 2 Gansu University of Chinese Medicine; 3 Child Healthcare Department, Gansu Provincial Maternity and Child-care Hospital
关键词:
胃癌; SGC-7901细胞; 凋亡; COX-2; Survivin; XIAP; p53; 阿魏酸
Keywords:
gastric cancer;  SGC-7901 cells;  apoptosis;  COX-2;  Survivin;  XIAP;  p53;  ferulic acid
分类号:
R285
文献标志码:
A
摘要:
目的：探讨阿魏酸对人胃癌SGC-7901细胞增殖的影响及其对凋亡相关基因表达的影响。方法：不同浓度(0、1.25、2.5、5、7.5、10、12.5 mg/mL)阿魏酸作用体外培养人胃癌SGC-7901细胞24、48、72小时后，MTT法检测细胞增殖；不同浓度(0、5、7.5、10 mg/mL)阿魏酸作用SGC-7901细胞48小时，流式细胞仪检测细胞凋亡，RT-qPCR和免疫印迹法(Western-blot)检测环氧化酶2(COX-2)、生存素(Survivin)、X连锁凋亡抑制蛋白(XIAP)和p53的mRNA和蛋白表达。结果：与对照组比较，7.5、10、12.5 mg/mL阿魏酸使SGC-7901细胞的OD值降低，5、7.5、10 mg/mL阿魏酸作用SGC-7901细胞48小时后均能诱导其凋亡，5、7.5、10 mg/mL阿魏酸均能上调p53的mRNA和蛋白表达，下调COX-2、Survivin和XIAP的mRNA和蛋白表达。结论：阿魏酸能有效抑制SGC-7901细胞增殖，并能诱导其凋亡，阿魏酸能上调胃癌细胞p53的mRNA和蛋白表达，下调COX-2、Survivin和XIAP的mRNA和蛋白表达，发挥抗肿瘤作用。
Abstract:
Objective: To discuss the effects of ferulic acid on the proliferation of human gastric cancer SGC-7901 cells and its influence on the expression of apoptosis-related gene. Methods: Different concentrations of ferulic acids were used to intervene human gastric cancer SGC-7901 cells cultured in vitro, after acting on for 24, 48 and 72 hours, MTT method was used to detect cellular proliferation; after different concentrations(0, 5, 7.5 and 10 mg/mL) of ferulic acids acting on SGC-7901 cells for 48 hours, flow cytometry was adopted to detect cellular apoptosis, RT-q PCR and Western-blot were uased to measure mRNA and protein expressions of COX-2, Survivin, XIAP and p53. Results: Compared with the control group, ferulic acids in the concentrations of 7.5, 10 and 12.5 mg/mL could decrease OD values of SGC-7901 cells, ferulic acids in the concentrations of 5, 7.5 and 10 mg/mL after acting on SGC-7901 cells for 48 hours could induce cellular apoptosis, ferulic acids in the concentrations of 5, 7.5 and 10 mg/mL could increase mRNA and protein expression of p53, decrease mRNA and protein expressions of COX-2, Survivin and XIAP. Conclusion: Ferulic acid could effectively inhibit the proliferation of SGC-7901 cells, induce its apoptosis, it could increase mRNA and protein expression of gastric cancer p53, decrease mRNA and protein expression of COX-2, Survivin and XIAP, and produce anti-tumor effects.

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备注/Memo

备注/Memo:
收稿日期：2018-08-15 *基金项目：甘肃省自然科学基金B类计划项目(编号 1212RJZA083)。 作者简介：牛媛瑕(1985—)，女，主管检验师。研究方向：临床检验。 △通讯作者：徐燕(1978—)，女，硕士学位，副编审。研究方向：中医编辑。
更新日期/Last Update: 2019-01-15