[1]白兆琴,王惠霞,王宏伟△.黄芩苷对胃癌SGC-7901细胞TLR8、HIF-1α、PDGFβ和pten表达的影响[J].西部中医药,2017,30(08):12-16.
 BAI Zhaoqin,WANG Huixia,WANG Hongwei.The Effects of Baicalin on the Expression of TLR8, HIF-1α, PDGFβ and pten of Gastric Cancer SGC-7901 Cells[J].Western Journal of Traditional Chinese Medicine,2017,30(08):12-16.
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黄芩苷对胃癌SGC-7901细胞TLR8、HIF-1α、PDGFβ和pten表达的影响
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
30
期数:
2017年08期
页码:
12-16
栏目:
方药·药理研究
出版日期:
2017-08-15

文章信息/Info

Title:
The Effects of Baicalin on the Expression of TLR8, HIF-1α, PDGFβ and pten of Gastric Cancer SGC-7901 Cells
文章编号:
1004-6852(2017)08-0012-05
作者:
白兆琴1王惠霞2王宏伟3△
1 甘肃省肿瘤医院药学部,甘肃 兰州 730050; 2 兰州市第二人民医院药剂科; 3 甘肃中医药大学附属医院
Author(s):
BAI Zhaoqin1 WANG Huixia2 WANG Hongwei3△
1 Pharmaceutical Sciences Section of Gansu Provincial Cancer Hospital, Lanzhou 730050, China; 2 Pharmaceutical Preparation Section of the Second People′s Hospital of Lanzhou City; 3 University Hospital of Gansu Traditional Chinese Medicine
关键词:
胃癌黄芩苷TLR8HIF-1α血小板衍生生长因子β
Keywords:
gastric cancer baicalin TRL8 HIF-1α PDGFβ
分类号:
R96
文献标志码:
A
摘要:
目的:探讨不同浓度的黄芩苷对胃癌SGC-7901细胞增殖的抑制作用,以及对pten和survivin基因的影响。方法:以不同浓度(10、20、40、80、160、320 μmol/L)的黄芩苷处理胃癌SGC-7901细胞后,采用MTT法测定细胞活力;80、120、160 μmol/L的黄芩苷处理胃癌SGC-7901细胞后,采用RT-qPCR法检测Toll样受体8(TLR8)、低氧诱导因子(HIF-1α)、血小板衍生生长因子β(PDGF-β)和第10号染色体上磷酸酶和张力白同源缺失性基因(pten)的表达,Western-blot法分别检测胃癌细胞TLR8、HIF-1α、PDGF-β和pten蛋白的表达。结果:不同浓度的黄芩苷均能抑制细胞增殖,RT-qPCR法检测显示TLR8、HIF-1α、PDGF-β基因表达下调,pten基因表达上调。结论:不同浓度的黄芩苷可抑制胃癌细胞增殖,并能上调pten和下调TLR8、HIF-1α、PDGF-βmRNA及蛋白的表达。
Abstract:
Objective: To discuss the inhibiting effects of baicalin with different concentrations on the proliferation of gastric cancer SGC-7901 cell, and its influence on the gene pten and survivin. Methods: Cellular activity was measured by adopting MTT method after dealing SGC-7901 cell with baicalin with different concentrations (10, 20, 40, 80, 160 and 320 μmol/L); the expressions of TLR8, HIF-1α, PDGFβ and pten were detected by RT-qPCR method after dealing SGC-7901 cell with baicalin with different concentrations (80, 120 and 160 μmol/L); the expressions of TLR8, HIF-1α, PDGFβ and pten were detected by Western-blot method respectively. Results: Baicalin with different concentrations could inhibit cellular proliferation, RT-qPCR method showed that the expressions of TLR8, HIF-1α and PDGFβ down regulated, while pten expression up regulated. Conclusion: Baicalin with different concentrations could inhibit gastric cancer cellular proliferation, and it could up regulate pten expressions and down regulate the expressions of TLR8, HIF-1α and PDGFβ mRNA and protein.

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备注/Memo

备注/Memo:
收稿日期:2017-02-25 *基金项目:2014甘肃省科技厅B类计划项目(编号 148RJZA073);2013甘肃省卫生行业管理项目(编号GWGL2013-40)。 作者简介:白兆琴(1970—),女,副主任药师。研究方向:临床药学。 △通讯作者:王宏伟(1977—),男,副主任检验师。研究方向:临床检验。
更新日期/Last Update: 2017-08-15