[1]王林凤,魏国玲,康毅.蒲地兰颗粒的质量控制[J].西部中医药,2014,27(04):17-20.
 WANG Linfeng,WEI Guoling,KANG Yi.Quality Control of PuDiLan Granules[J].Western Journal of Traditional Chinese Medicine,2014,27(04):17-20.
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蒲地兰颗粒的质量控制
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《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
27
期数:
2014年04期
页码:
17-20
栏目:
方药·质量分析
出版日期:
2014-04-15

文章信息/Info

Title:
Quality Control of PuDiLan Granules
文章编号:
1004-6852(2014)04-0017-04
作者:
王林凤魏国玲康毅
陕西中医学院附属医院,陕西 咸阳 712000
Author(s):
WANG Linfeng WEI Guoling KANG Yi
Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
关键词:
蒲地兰颗粒质量控制薄层色谱高效液相色谱法黄芩苷
Keywords:
PuDiLan granules quality control thin-layer chromatography high liquid chromatographybaicalin
分类号:
R284
文献标志码:
A
摘要:
目的:建立蒲地兰颗粒的质量控制方法。方法:采用薄层色谱法对黄芩进行定性鉴别,采用HPLC法对黄芩中的黄芩苷进行含量测定。色谱柱为Thermo C18(250 mm×4.6 mm,5 μm),流动相为甲醇-0.2%磷酸水溶液(47∶53),流速为1.0 mL/min,检测波长为280 nm。柱温为室温。结果:薄层色谱斑点清晰,阴性对照无干扰。黄芩苷在0.076~7.6 μg范围内线性关系良好(r=0.9999),平均回收率为99.5%,RSD=0.71%(n=6)。结论:本研究建立的TLC和HPLC方法操作简便,结果准确,重复性好,专属性强,可用于该制剂的质量控制。
Abstract:
Objective: To establish the method for quality control of PuDiLan granules. Methods: HuangQin (Scutellaria baicalensis) was identified qualitatively by TCL, the contents of baicalin in HuangQin were determined by HPLC. Chromatographic column: Thermo C18 (250 mm×4.6 mm, 5 μm), mobile phase: methanol-0.2% phosphoric acid solution (47:53), flow rate was 1.0mL/min, detection wavelength: 280nm, room temperature. Results: The spots of TLC were distinguished and clear. Baicalin showed better linear relationship in the range between 0.076 and 7.6 μg (r=0.9999), average recovery was 99.5%, RSD=0.71%(n=6). Conclusion: TLC and HPLC method established in the study, simple, accurate, reproducible and specific, could be used to quality control of the preparation.

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备注/Memo

备注/Memo:
收稿日期:2013-11-20作者简介:王林凤(1973—),女,主管药师。研究方向:中药质量标准的研究。
更新日期/Last Update: 2014-04-15