[1]程阔菊,彭雷,朱丹,等.柴胡疏肝散对胃肠功能紊乱大鼠模型胃肠道Cajal间质细胞凋亡的影响[J].西部中医药,2020,33(01):21.[doi:10.12174/j.issn.1004-6852.2020.01.06]
　CHENG Kuoju,PENG Lei,ZHU Dan,et al.Effects of Chaihu Shugan Powder on the Apoptosis of Gastrointestinal ICCs in Rats with Gastrointestinal Dysfunction[J].Western Journal of Traditional Chinese Medicine,2020,33(01):21.[doi:10.12174/j.issn.1004-6852.2020.01.06]
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柴胡疏肝散对胃肠功能紊乱大鼠模型胃肠道Cajal间质细胞凋亡的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年01期
页码:
21
栏目:
出版日期:
2020-01-15

文章信息/Info

Title:
Effects of Chaihu Shugan Powder on the Apoptosis of Gastrointestinal ICCs in Rats with Gastrointestinal Dysfunction
文章编号:
1004-6852(2020)01-0021-04
作者:
程阔菊1，彭雷1，朱丹1，赵婷1，程俊2，王娜2，罗云1△
1 达州市中西医结合医院，四川 达州 635000； 2 西南医科大学心肌电生理学研究室
Author(s):
CHENG Kuoju1, PENG Lei1, ZHU Dan1, ZHAO Ting1, CHENG Jun2, WANG Na2, LUO Yun1△
1 Dazhou City Integrative Medicine Hospital, Dazhou 635000, China; 2 Myocardial Electrophysiology Lab, Southwest Medical University
关键词:
胃肠功能紊乱; Cajal间质细胞; 凋亡; 柴胡疏肝散; 大鼠
Keywords:
gastrointestinal dysfunction;  ICCs;  apoptosis;  Chaihu Shugan powder;  rats
分类号:
R256.3
DOI:
10.12174/j.issn.1004-6852.2020.01.06
文献标志码:
A
摘要:
目的:探讨柴胡疏肝散对胃肠功能紊乱大鼠模型胃肠道Cajal间质细胞凋亡的影响。方法:将8周龄SD雄性大鼠随机分为sham组(对照组)、FGID组(模型组)、sham+药物组及FGID+药物组各15只。利用腹腔注射利血平建立胃肠功能紊乱大鼠模型，造模同时sham+药物组及FGID+药物组予柴胡疏肝散汤剂治疗，利用免疫细胞化学和TUNEL法检测柴胡疏肝散对模型大鼠胃肠道Cajal间质细胞凋亡的影响。结果:sham组内见较多Cajal间质细胞，极少见Cajal间质细胞凋亡；sham+药物组与sham组比较差异无统计学意义(P＞0.05)；FGID组Cajal间质细胞发生明显凋亡且细胞数量明显减少(P＜0.05)；FGID+药物组内Cajal间质细胞数量及凋亡情况较FGID组明显改善(P＜0.05)。结论:柴胡疏肝散对正常大鼠Cajal间质细胞无影响，但能明显减轻胃肠功能紊乱模型大鼠胃肠道Cajal间质细胞的凋亡。
Abstract:
Objective: To discuss the effects of Chaihu Shugan powder on gastrointestinal interstitial cells of Cajal(ICCs) in rats with gastrointestinal dysfunction. Methods: SD male rats aged eight weeks were randomized into sham group (the control group), FGID group (the model group), sham + medicine group and FGID + medicine group, 15 rats each group. Peritoneal injection of reserpine was used to establish rats model with gastrointestinal dysfunction, simultaneously, sham + medicine group and FGID+medicine group were treated by the decoction of Chaihu Shugan powder, immunocytochemistry and TUNEL method were adopted to detect the effects of Chaihu Shugan powder on the apoptosis of gastrointestinal ICCs. Results: More ICCs could be seen in sham group, scanty ICCs apoptosis could be seen; no significant difference could be found between sham+medicine group and sham group (P＞0.05); in FGID group, ICCs occured the apoptosis notaly and the number of the cells reduced remarkably(P＜0.05); the number and apoptosis conditions of ICCs in FGID + medicine group improved compared with FGID group remarkably(P＜0.05). Conclusion: Chaihu Shugan powder shows no effects on ICCs of normal rats, but could obviously reduce the apoptosis of ICCs in rats with gastrointestinal dysfunction.

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[1]景晓慧.吴茱萸敷脐联合电针对全子宫切除术后患者胃肠功能恢复的影响[J].西部中医药,2014,27(03):117.
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[2]李慧,田耀洲,陆敏 夏军权△.养阴和胃方对糖尿病胃轻瘫大鼠SCF/c-kit系统的影响[J].西部中医药,2017,30(12):9.
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备注/Memo

备注/Memo:
收稿日期：2018-11-23*基金项目：2015年四川省科技计划项目(2015JY0270)；2014年达州市科技计划项目(达市财建［2014］82号)。作者简介：程阔菊(1984—)，女，硕士学位，主管药师。研究方向：胃肠病学。△通讯作者：罗云(1964—)，男，博士学位，主任医师。研究方向：胃肠病学。
更新日期/Last Update: 2020-01-15