[1]王燕,谢亲建,马弢业,等.白刺果作用于白血病NB4细胞的机制研究[J].西部中医药,2021,34(04):28-30.[doi:10.12174/j.issn.2096-9600.2021.04.07]
　WANG Yan,XIE Qinjian,MA Taoye,et al.Study on the Mechanism of Baiciguo Acting on Leukemia NB4 Cells[J].Western Journal of Traditional Chinese Medicine,2021,34(04):28-30.[doi:10.12174/j.issn.2096-9600.2021.04.07]
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白刺果作用于白血病NB4细胞的机制研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年04期
页码:
28-30
栏目:
出版日期:
2021-04-15

文章信息/Info

Title:
Study on the Mechanism of Baiciguo Acting on Leukemia NB4 Cells
作者:
王燕1, 谢亲建2,3, 马弢业4, 丁晓煜2, 王爱昕2, 潘铭1
1.武威市人民医院,甘肃 武威 733000
2.武警甘肃省总队医院
3.兰州大学药学院
4.西北民族大学第二附属医院
Author(s):
WANG Yan1, XIE Qinjian2,3, MA Taoye4, DING Xiaoyu2, WANG Aixin2, PAN Ming1
1.Wuwei People′s Hospital, Wuwei 733000, China
2.Gansu Corps Hospital of CAPF
3.Pharmacy School, Lanzhou University
4.Second Provincial People′s Hospital of Gansu
关键词:
白刺果; 白血病; NB4细胞; 线粒体
Keywords:
leukemia; NB4 cells; mitochondrion
分类号:
R285
DOI:
10.12174/j.issn.2096-9600.2021.04.07
摘要:
目的研究白刺果（Nitraria L.）对白血病NB4细胞的抑制作用。 方法采用MTT法研究白刺果对NB4细胞抑制作用，通过流式细胞仪进一步确定白刺果对NB4细胞的抑制周期，采用免疫印迹法探讨白刺果对相关蛋白的表达。 结果白刺果对白血病NB4细胞有明显的抑制作用，呈时间和浓度梯度依赖性，阻止细胞周期G2，且对半胱氨酸天冬氨酸蛋白酶3（Pro-caspase3）具有上调作用，下调抗凋亡蛋白B细胞淋巴瘤（B cell lymphoma/leukemia-2，Bcl-2）和细胞色素C（cytochrome C，C-myc）水平。 结论白刺果对白血病NB4细胞具有一定的抑制作用，该抑制作用通过激活线粒体途径Pro-caspase3及Bcl-2和C-myc起作用，其他途径有待于我们进一步研究。
Abstract:
ObjectiveTo study the inhibitory effects of Baiciguo (Nitraria fruit) on leukemia NB4 cells. MethodsMTT assay was used to study the inhibitory effects of Baiciguo on leukemia NB4 cells, flow cytometry was adopted to further ensure the inhibitory cycle of Baiciguo on NB4 cells, and Western blotting to explore the expressions of the related protein in Baiciguo. ResultsBaiciguo showed notable inhibitory effects on leukemia NB4 cells, in time and concentration gradient dependent, it could block cell cycle G2, up regulate Pro-caspase3, and down regulate Bcl-2 and C-myc. ConclusionBaiciguo has a certain inhibitory effects on leukemia NB4 cells by activating the mitochondrial pathway Pro-caspase3, Bcl-2 and C-myc proteins, and other pathways need to be further studied.

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备注/Memo

备注/Memo:
王燕（1982—），女，主治医师。研究方向：血液病的诊治。中央高校基金（31920180098）；甘肃省自然科学基金（1606RJZH153）。
更新日期/Last Update: 2021-04-15