[1]陈玮钰,毛德文,王娜,等.基于数据挖掘探析急性肝衰竭动物模型的应用[J].西部中医药,2025,38(10):36-42.[doi:10.12174/j.issn.2096-9600.2025.10.08]
　CHEN Weiyu,MAO Dewen,WANG Na,et al.Application of ALF Animal Model Based on Data Mining[J].Western Journal of Traditional Chinese Medicine,2025,38(10):36-42.[doi:10.12174/j.issn.2096-9600.2025.10.08]
点击复制

基于数据挖掘探析急性肝衰竭动物模型的应用()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年10期
页码:
36-42
栏目:
二次研究
出版日期:
2025-10-15

文章信息/Info

Title:
Application of ALF Animal Model Based on Data Mining
作者:
陈玮钰1, 毛德文2, 王娜2, 王秀峰2, 陶然1, 舒发明2
1.广西中医药大学，广西 南宁 530222
2.广西中医药大学第一附属医院，广西 南宁 530023
Author(s):
CHEN Weiyu1, MAO Dewen2, WANG Na2, WANG Xiufeng2, TAO Ran1, SHU Faming2
1.Guangxi University of Chinese Medicine, Nanning 530001, China
2.the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
关键词:
急性肝衰竭; 动物模型; 数据挖掘; 作用机制
Keywords:
acute liver failure; animal models; data mining; mechanism
分类号:
R259
DOI:
10.12174/j.issn.2096-9600.2025.10.08
文献标志码:
A
摘要:
目的探析急性肝衰竭（acute liver failure，ALF）动物模型的应用，为今后基础研究提供方法学参考。 方法以ALF合并动物模型为主题词，检索中、英文数据库，筛选近7年收录的ALF相关动物实验期刊文献，整理归纳模型中涉及的动物基本资料、造模方法和周期、造模前后的行为学变化、检测指标及方法、中药干预措施等内容，并建立数据库进行统计分析和可视化处理。 结果共纳入222篇文献，ALF模型中应用最多的是C57BL/6小鼠和SD大鼠，性别选择以雄性为主；建模方法多为化学性造模法，以腹腔注射D-GaIN联合LPS为主；模型建立成功与否的评价标准及检测方法包括行为学变化、组织病理学以及血清生化法、PCR法、WB法、ELISA法和IHC法检测指标等；中药方剂干预药物以解毒化瘀颗粒、清肠利肝方、茵陈四苓颗粒和大黄、赤芍注射液最常见。 结论目前ALF动物实验研究方向与炎症反应、氧化应激、细胞凋亡、内质网应激密切相关，所应用的动物模型种类较多，但缺乏系统且合理的评价标准，模型构建和应用中存在一定问题。
Abstract:
Objective: To serve as a methodological reference for future basic research by discussing the application of ALF animal models. MethodsAcute liver failure and animal models were used as keywords to search the English and Chinese databases for screening journals on animal experiments of ALF in the recent seven years. We sorted and concluded the basic information of animals involved in the models, modeling methods and cycles, behavioral changes before and after modeling, detection indicators and methods, and herbal intervention measures, established the database for statistical analysis and visualization. ResultsThe study included 222 papers, animal models employing C57BL/6 mice and SD rats were used to induce ALF, predominantly male; the models with ALF were established primarily by intraperitoneal injection of D-galactosamine (D-GalN) in combination with lipopolysaccharide (LPS); the evaluation criteria and detection methods for successful modeling contained behavioral changes, histopathology, serological biochemical method, PCR, WB, ELISA and IHC; the prescriptions for the intervention covered detoxification and stasis-resolving granules, intestine and liver clearing formulation, Yinchen Siling granules, and Dahuang injection, as well as Chishao injection. ConclusionAt present, the research on ALF animal models is critically linked to inflammatory reaction, oxidative stress, cellular apoptosis, and endoplasmic reticulum stress, various types of animal models were employed, but there was a lack of systematic and reasonable evaluation criteria, and some problems in model construction and application existed.

相似文献/References:

[1]甘雨,马进△,袁媛,等.线栓法制备SD大鼠局灶性脑缺血再灌注模型的实践与评价[J].西部中医药,2018,31(07):12.
　GAN Yu,MA Jin,YUAN Yuan,et al.Practice and Evaluation of Focal Cerebral Ischemia Reperfusion Rat Model Prepared by Middle Cerebral Artery Occlusion[J].Western Journal of Traditional Chinese Medicine,2018,31(10):12.
[2]郭燕,逯克娜,林寒梅.补肾活血类中药对黄体功能不全大鼠模型干预研究的系统评价[J].西部中医药,2025,38(09):75.[doi:10.12174/j.issn.2096-9600.2025.09.14]
　GUO Yan,LU Ke?na,LIN Hanmei.Systematic Evaluation of the Intervention of Kidney-tonifying Blood-activating Herbs in Rat Models with Luteal Phase Deficiency[J].Western Journal of Traditional Chinese Medicine,2025,38(10):75.[doi:10.12174/j.issn.2096-9600.2025.09.14]
[3]支文冰,刘洋,李晔,等.哮喘实验动物模型的建立及评价[J].西部中医药,2025,38(11):61.[doi:10.12174/j.issn.2096-9600.2025.11.10]
　ZHI Wenbing,LIU Yang,LI Ye,et al.Establishment and Evaluation of Animal Models of Asthma[J].Western Journal of Traditional Chinese Medicine,2025,38(10):61.[doi:10.12174/j.issn.2096-9600.2025.11.10]
[4]蒙帅杰,蒋越,王权胜,等.补肾活血类中药对少弱精子症模型生殖激素水平干预研究的系统评价?[J].西部中医药,2021,34(11):94.[doi:10.12174/j.issn.2096-9600.2021.11.20]
　MENG Shuaijie,JIANG Yue,WANG Quansheng,et al.Systematic Evaluation on the Intervention of Kidney-tonifying Blood-activating Herbs on Reproductive Hormone Levels in Oligoasthenospermia Model[J].Western Journal of Traditional Chinese Medicine,2021,34(10):94.[doi:10.12174/j.issn.2096-9600.2021.11.20]
[5]陈丹丹,董博,欧国峰,等.抗疏强骨颗粒对去卵巢模型大鼠血清ALP及TRACP5b的影响[J].西部中医药,2021,34(05):22.[doi:10.12174/j.issn.2096-9600.2021.05.06]
　CHEN Dandan,DONG Bo,OU Guofeng,et al.Influence of Anti-osteoporosis Strengthening Bone Granules on Serum ALP and TRACP5b in Ovariectomized Rats[J].Western Journal of Traditional Chinese Medicine,2021,34(10):22.[doi:10.12174/j.issn.2096-9600.2021.05.06]
[6]高国莉.针刀对肌性斜颈模型兔胸锁乳突肌中calpain-1、CD45、MMP-2、TIMP-2蛋白表达的影响[J].西部中医药,2024,37(04):13.[doi:10.12174/j.issn.2096-9600.2024.04.03]
　GAO Guoli.Influence of Needle-knife on the Expressions of calpain-1, CD45, MMP-2 and TIMP-2 Protein in Cleidomastoid of CMT Rabbit Models[J].Western Journal of Traditional Chinese Medicine,2024,37(10):13.[doi:10.12174/j.issn.2096-9600.2024.04.03]
[7]张文瑞,杨爽,吕新亮.类风湿关节炎中医实验动物模型研究进展[J].西部中医药,2024,37(04):106.[doi:10.12174/j.issn.2096-9600.2024.04.21]
　ZHANG Wenrui,YANG Shuang,LYU Xinliang.Research Progress on Animal Model with Rheumatoid Arthritis in Traditional Chinese Medicine[J].Western Journal of Traditional Chinese Medicine,2024,37(10):106.[doi:10.12174/j.issn.2096-9600.2024.04.21]
[8]檀盛,邓强,李建花,等.建立椎间盘退变动物模型的研究进展[J].西部中医药,2024,37(07):103.[doi:10.12174/j.issn.2096-9600.2024.07.24]
　TAN Sheng,DENG Qiang,LI Jianhua,et al.Research Progress in Establishing Animal Models of Intervertebral Disc Degeneration[J].Western Journal of Traditional Chinese Medicine,2024,37(10):103.[doi:10.12174/j.issn.2096-9600.2024.07.24]
[9]王丹丹,覃骊兰.荨麻疹中医证候动物模型研究进展[J].西部中医药,2025,38(01):105.[doi:10.12174/j.issn.2096-9600.2025.01.20]
　WANG Dandan,QIN Lilan.Research Progress of Animal Models of TCM Syndromes of Urticaria[J].Western Journal of Traditional Chinese Medicine,2025,38(10):105.[doi:10.12174/j.issn.2096-9600.2025.01.20]

备注/Memo

备注/Memo:
陈玮钰（1996—），男，在读博士研究生。研究方向：肝脏疾病的防治。国家自然科学基金（81960841，82060848）；广西自然科学基金（2020GXNSFAA297070）；广西研究生教育创新计划资助项目（YCSZ2022017、YCBZ2024149）；广西中医药多学科交叉创新团队（GZKJ2306）。
更新日期/Last Update: 2025-10-15