[1]赵妮,邓毅△,刘靓,等.正交实验优选甘草总黄酮和总皂苷提取工艺[J].西部中医药,2016,29(02):34-36.
　ZHAO Ni,DENG Yi,LIU Liang,et al.Optimization of Extraction Technology of Total Flavonoids and Total Saponins from GanCao by Orthogonal Test[J].Western Journal of Traditional Chinese Medicine,2016,29(02):34-36.
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正交实验优选甘草总黄酮和总皂苷提取工艺()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年02期
页码:
34-36
栏目:
出版日期:
2016-02-15

文章信息/Info

Title:
Optimization of Extraction Technology of Total Flavonoids and Total Saponins from GanCao by Orthogonal Test
文章编号:
1004-6852(2016)02-0034-03
作者:
赵妮1，邓毅1△，刘靓2，董金香2，曼琼2
1甘肃中医药大学，甘肃 兰州 730000；2 甘肃省中(藏)药化学与质量研究省级重点实验室
Author(s):
ZHAO Ni1, DENG Yi1△, LIU Liang2, DONG Jinxiang2, MAN Qiong2
1 Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;2 Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province
关键词:
甘草; 总黄酮; 总皂苷; 正交实验; 提取工艺
Keywords:
GanCao;  total flavonoids;  total saponins;  orthogonal test;  extraction technology
分类号:
R284.2
文献标志码:
A
摘要:
目的：优化甘草总黄酮和总皂苷的超声提取工艺。方法：以提取温度、提取时间、乙醇浓度、料液比为考察因素，以总黄酮和总皂苷含量为综合评分作为评价指标，优选出最佳提取工艺。结果：最佳提取工艺：提取温度为50℃，提取时间为45分钟，乙醇浓度为60%，料液比为1∶10。结论：该提取工艺简便、快捷，可用于甘草总黄酮、总皂苷的同时提取，为甘草进一步研究与利用奠定基础。
Abstract:
Objective: To optimize ultrasonic extraction technology of total flavonoids and total saponins from GanCao (Glycyrrhiza uralensis Fisch.). Methods: The best extraction technology was optimized by taking extraction temperature, extraction time, the concentrations of ethanol and solid-liquid ratio as the factors, the contents of total flavonoids and total saponins as evaluation indexes for comprehensive assessment. Results: The best extraction technology was: extraction temperature: 50℃, extraction time: 45 minutes, the concentrations of ethanol: 60%, solid-liquid ratio: 1: 10. Conclusion: The extraction technology, simple, convenient and rapid, could be used for simultaneous extraction of total flavonoids and total saponins from GanCao, which could lay foundation for further study and utilization of GanCao.

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备注/Memo

备注/Memo:
*基金项目：国家自然科学基金项目（编号81360633）。作者简介：赵妮(1988—)，女，在读硕士研究生。研究方向：中药及复方应用研究。△通讯作者：邓毅(1964—)，男，硕士研究生导师，教授，中药学科学术带头人。研究方向：中药学教学、中药性能与配伍应用及中药不良反应的研究。
更新日期/Last Update: 2016-02-15