[1]周睿娴,王光义,陈睿,等.葛根素抑制间充质干细胞成脂肪分化的作用及机制[J].西部中医药,2020,33(03):34-38.[doi:10.12174/j.issn.1004-6852.2020.03.09]
　ZHOU Ruixian,WANG Guangyi,CHEN Rui,et al.Effects and Mechanism of Puerarin in Inhibiting Adipogenic Differentation of Mesenchymal Stem Cells[J].Western Journal of Traditional Chinese Medicine,2020,33(03):34-38.[doi:10.12174/j.issn.1004-6852.2020.03.09]
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葛根素抑制间充质干细胞成脂肪分化的作用及机制
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年03期
页码:
34-38
栏目:
出版日期:
2020-03-15

文章信息/Info

Title:
Effects and Mechanism of Puerarin in Inhibiting Adipogenic Differentation of Mesenchymal Stem Cells
文章编号:
1004-6852(2020)03-0034-04
作者:
周睿娴，王光义，陈睿，孙苏闻
贵州医科大学附属医院针灸科，贵州 贵阳 550004
Author(s):
ZHOU Ruixian, WANG Guangyi, CHEN Rui, SUN Suwen
Acupuncture Department, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China
关键词:
成脂肪分化; 间充质干细胞; wnt信号通路; 葛根素; 实验研究
Keywords:
adipogenic differentiation;  mesenchymal stem cells;  wnt signaling pathway;  puerarin;  xperimental research
分类号:
R622
DOI:
10.12174/j.issn.1004-6852.2020.03.09
摘要:
目的：探讨葛根素对骨髓间充质干细胞(bone-marrow derived mesenchymal stem cells，BMSCs)成脂肪分化的抑制作用与分子机制。方法：Percoll梯度密度离心法分离BMSCs；采用BMSCs特异性无血清培养基进行培养及传代；流式细胞术鉴定BMSCs表面特异性抗原表达；0.2 μM和0.5 μM葛根素分别联合成脂肪分化诱导培养基BMSCs向脂肪细胞分化；油红染液在第12天鉴定脂肪细胞形成情况；real-time PCR和western blot分别鉴定成脂肪分化相关因子PPAR-γ、CEBP-α及CEBP-β的表达；观察0.2 μM和0.5 μM葛根素对成脂肪分化相关Wnt信号通路的影响。结果：葛根素可显著抑制BMSCs向脂肪细胞分化，并呈浓度依赖性(P＜0.05)，成脂肪诱导分化12天时，未加葛根素的BMSCs中PPAR-γ、CEBP-α及CEBP-β表达水平高于加葛根素组；葛根素组BMSCs胞内β-cateinin表达受到明显抑制。结论：葛根素可以明显抑制BMSCs向成脂肪细胞转化。
Abstract:
Objective: To explore the inhibitory effects of puerarin on adipogenic differentiation of bone marrow mesenchymal stem cells(BMSCs) and its molecular mechanism. Methods: Percoll gradient density centrifugation method was used to separate BMSCs; BMSCs specific serum-free medium for the cultivation and the subculture; flow cytometry to identify the expressions of BMSCs surface specific antigen; 0.2 μM and 0.5 μM puerarin combined with adipogenic differentiation - induced medium respectively to induce adipogenic differentiation of BMSCs respectively; oil red dye solution to identify the formation of adipocytes at the 12th day; real - time PCR and western blot were adopted to measure the expressions of PPAR-γ, CEBP - α and CEBP-β in mRNA and protein levels; to observe the influence of 0.2 μM and 0.5 μM puerarin on adipogenic differentiation related wnt signaling pathway. Results: Puerarin could inhibit adipogenic differentiation of BMSCs remarkably, in a dose - dependent manner (P＜0.05), After inducing for 12 days, the expressions of PPAR-γ, CEBP - α and CEBP-β in BMSCs without adding puerarin were higher than these of puerarin group; the expressions of β-cateinin in BMSCs were inhibited obviously in puerarin group. Conclusion: Puerarin could notably inhibit adipogenic differentiation of BMSCs.

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备注/Memo

备注/Memo:
收稿日期：2019-06-16*基金项目：贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY2017-117)。作者简介：周睿娴(1986—)，女，硕士学位，主治医师。研究方向：脑
更新日期/Last Update: 2020-03-15