[1]温明韬,梁学振,许波,等.基于网络药理学探讨杜仲-牛膝治疗股骨头坏死的分子机制[J].西部中医药,2021,34(08):76-83.[doi:10.12174/j.issn.2096-9600.2021.08.17]
　WEN Mingtao,LIANG Xuezhen,XU Bo,et al.Molecular Mechanism of Duzhong-niuxi in Treating Osteonecrosis of the Femoral Head Based on Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2021,34(08):76-83.[doi:10.12174/j.issn.2096-9600.2021.08.17]
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基于网络药理学探讨杜仲-牛膝治疗股骨头坏死的分子机制
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年08期
页码:
76-83
栏目:
出版日期:
2021-08-15

文章信息/Info

Title:
Molecular Mechanism of Duzhong-niuxi in Treating Osteonecrosis of the Femoral Head Based on Network Pharmacology
作者:
温明韬, 梁学振, 许波, 刘金豹, 杨振勋, 李刚
山东中医药大学，山东 济南 250355
Author(s):
WEN Mingtao, LIANG Xuezhen, XU Bo, LIU Jinbao, YANG Zhenxun, LI Gang
Shandong University of Traditional Chinese Medicine, Jinan 250355, China
关键词:
杜仲; 牛膝; 股骨头坏死; 网络药理学; 分子机制
Keywords:
ONFH; network pharmacology; molecular mechanism
分类号:
R684
DOI:
10.12174/j.issn.2096-9600.2021.08.17
摘要:
目的探讨“杜仲-牛膝”药对治疗股骨头坏死（osteonecrosis of the femoral head，ONFH）潜在有效成分和作用分子机制。 方法利用中药系统药理学数据库（traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP）完成对“杜仲-牛膝”药对的活性成分和药物活性成分靶点的收集、筛选和预测；通过疾病数据库收集股骨头坏死相关的作用靶点；将获得的关键靶点基因上传至STRING数据库进行分析，并通过可视化软件构建分析蛋白相互作用网络（protein-protein interaction，PPI）对关键靶点蛋白进行富集分析。 结果共获得杜仲、牛膝共同主要有效活性成分45个、140个药物靶点蛋白和1159个股骨头坏死相关基因；药物与疾病共有靶点47个；PPI网络分析IL-6、TNF、TP53、MAPK1、VEGFA、EGF、JUN、EGFR、NOS3、CCL2等可能是“杜仲-牛膝”治疗ONFH的潜在关键靶点；其通过参与成骨成血管、细胞的分化与凋亡、炎症反应及全身应激反应等主要作用通路以调控ONFH疾病的治疗过程。 结论“杜仲-牛膝”药对治疗股骨头坏死的作用具有多成分、多靶点、多功能、多调控通路的特点。初步揭示了其作用的关键靶点、主要信号通路和涉及的生物学过程，为机制的后续研究及实验验证提供了明确的依据与方向。
Abstract:
ObjectiveTo explore the potential active ingredients and molecular mechanism of Duzhong-niuxi in the treatment of ONFH. MethodsTCMSP was used to collect, screen and predict active ingredients of Duzhong-niuxi and the targets of active ingredients contained in drug pair; the targets related to ONFH were collected through disease database; and the obtained key target genes were uploaded to STRING database and analyzed, PPI was constructed and analyzed via visualization software; the key target proteins were collected to perform the enrichment analysis. ResultsAltogether 45 common major active ingredients of Duzhong and Niuxi, 140 drug target protein and 1159 ONFH-related genes were obtained; there are 47 common targets of drugs and disease; PPI network analysis showed that IL-6, TNF, TP53, MAPK1, VEGFA, EGF, JUN, EGFR, NOS3, CCL2 and others might be potential key targets of Duzhong-niuxi in treating ONFH; they regulated the therapeutic process of ONFH by participating in the main pathways of osteogenesis and angiogenesis, cell differentiation and apoptosis, inflammatory response and systemic stress response. ConclusionTherapeutic effects of drug pair of Duzhong-niuxi has the characteristics of multi-component, multi-target, multi-functional and multi regulatory pathways. The study preliminarily reveals the key targets, main signaling pathways and biological processes involved, in order to provide the clear reference and direction for the following study on the study and experi-mental verification.

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[3]石威,张俊忠,解鹏超,等.基于网络药理学和分子对接探析淫羊藿-牛膝药对治疗股骨头坏死的作用机制[J].西部中医药,2022,35(04):35.[doi:10.12174/j.issn.2096-9600.2022.04.09]
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备注/Memo

备注/Memo:
温明韬（1995—），男，在读硕士研究生。研究方向：骨与关节创伤的基础与临床研究。
更新日期/Last Update: 2021-08-15