[1]陈懿榕,阙任烨,刘进锴,等.柴胡皂苷d对大鼠肝星状细胞TGFβ-Smad信号通路mRNA表达的影响[J].西部中医药,2022,35(08):15-19.[doi:10.12174/j.issn.2096-9600.2022.08.04]
　CHEN Yirong,QUE Renye,LIU Jinkai,et al.Influence of Saikosaponins-d on the Expressions of TGFβ-Smad Signal Path mRNA in Rat Hepatic Stellate Cells[J].Western Journal of Traditional Chinese Medicine,2022,35(08):15-19.[doi:10.12174/j.issn.2096-9600.2022.08.04]
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柴胡皂苷d对大鼠肝星状细胞TGFβ-Smad信号通路mRNA表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年08期
页码:
15-19
栏目:
出版日期:
2022-08-15

文章信息/Info

Title:
Influence of Saikosaponins-d on the Expressions of TGFβ-Smad Signal Path mRNA in Rat Hepatic Stellate Cells
作者:
陈懿榕1, 阙任烨2, 刘进锴3, 林柳兵1, 李勇1
1.上海中医药大学附属市中医医院，上海 200071
2.上海中医药大学附属上海市中西医结合医院
3.第二军医大学附属东方肝胆外科医院
Author(s):
CHEN Yirong1, QUE Renye2, LIU Jinkai3, LIN Liubing1, LI Yong1
1.Shanghai City Hospital of Traditional Chinese Medicine, Shanghai 200041, China
2.Shanghai TCM-integrated Hospital, Shanghai University of TCM
3.Eastern Hepatobiliary Surgery Hospital affiliated to the Second Military Medical University
关键词:
肝纤维化; 柴胡皂苷d; 雌二醇; 肝星状细胞; TGF-Smad信号通路; 动物实验; 大鼠
Keywords:
liver fibrosis; SSd; estradiol; HSC-T6; TGF-Smad signal pathway; animal experiment; rat
分类号:
R575.2
DOI:
10.12174/j.issn.2096-9600.2022.08.04
摘要:
目的观察柴胡皂苷d（saikosaponins-d，SSd）对肝星状细胞TGFβ1、Smad3/7mRNA表达的影响。 方法采用大鼠肝星状细胞（hepatic stellate cell-T6，HSC-T6）为模型，采用药物进行干预后加入H2O2诱导氧化应激，采用RT-PCR实验方法检测TGFβ1、Smad3/7 mRNA表达。 结果与空白组比较，模型组TGFβ1、Smad3表达增强，Smad7表达减弱（P＜0.05）。与模型组比较，SSd组干预后HSC-T6细胞中TGFβ1、Smad3 mRNA水平降低，Smad7 mRNA水平增高（P＜0.05）。这种作用可被雌激素受体（estrogen receptor，ER）完全拮抗剂和ERβ拮抗剂阻滞。 结论SSd能下凋TGFβ1、Smad3 mRNA表达，上调 Smad7 mRNA的表达，对HSC-T6的TGFβ-Smad信号通路有明显的影响，可能是SSd对肝纤维化产生治疗作用的机制之一。
Abstract:
ObjectiveTo observe the effects of SSd on the expressions of TGFβ1, Smad3/7mRNA in hepatic stellate cells. MethodsRat HSC-T6 were adopted as the model, after drug intervention, H2O2 was added to induce oxidative stress, RT-PCR was used to detect the expressions of TGFβ1, Smad3/7mRNA. ResultsCompared with the blank group, the expression of TGFβ1 and Smad3 in the model group was enhanced, while the expression of Smad7 was weakened (P<0.05). Compared with the model group, the levels of TGFβ1 and Smad3 mRNA were decreased while the level of Smad7mRNA was elevated in HSC-T6 cells in SSd group after the intervention (P<0.05). The effects could be blocked by ER complete antagonist and ERβ antagonist. ConclusionSSd could down regulate the expressions of TGFβ1, Smad3 mRNA, up regulate the expressions of Smad7 mRNA, and it could affect TGFβ-Smad signal pathway in HSC-T6 signal pathway, which might be one of the mechanisms of SSd producing therapeutic effects on liver fibrosis.

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备注/Memo

备注/Memo:
陈懿榕（1992—），女，硕士学位。研究方向：血液病的临床与基础研究。国家自然科学基金面上项目（81573775，81873157）；上海市科委引导类项目（18401904000）；上海市青年科技英才扬帆计划（19YF1445200）；上海市卫健委中发办青年基金（2018LQ016）；上海中医药大学预算内项目（2021LK067）。
更新日期/Last Update: 2022-08-15