[1]邓毅,王艳,丁仁伟,等.甘肃乌拉尔甘草野生与栽培品内生菌的分离及鉴定*[J].西部中医药,2012,25(11):8-11.
　DENG Yi,WANG Yan,DING Ren-wei,et al.Identification and Separation of Endophytes in Wild and Cultivated Glycyrrhiza Uralensis from Gansu Province[J].Western Journal of Traditional Chinese Medicine,2012,25(11):8-11.
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甘肃乌拉尔甘草野生与栽培品内生菌的分离及鉴定*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
25
期数:
2012年11期
页码:
8-11
栏目:
出版日期:
2012-11-15

文章信息/Info

Title:
Identification and Separation of Endophytes in Wild and Cultivated Glycyrrhiza Uralensis from Gansu Province
文章编号:
1004-6852(2012)11-0008-04
作者:
邓毅1，王艳1，丁仁伟1，杨志军1，张艳萍2△
1 甘肃中医学院，甘肃 兰州 730000；2 甘肃中医学院附属医院
Author(s):
DENG Yi1, WANG Yan1, DING Ren-wei1, YANG Zhi-jun1, ZHANG Yan-ping2△
1 Gansu University of Chinese Medicine, Lanzhou 730000, China; 2 University Hospital of Gansu Traditional Chinese Medicine
关键词:
甘肃; 乌拉尔甘草; 野生栽培; 内生细菌; 内生真菌; 分离纯化; 种类鉴定
Keywords:
Gansu;  Glycyrrhiza Uralensis;  wild cultivation;  endophytic bacteria;  endophytic fungi;  isolation and purification;  species identification
分类号:
R282.7
文献标志码:
A
摘要:
目的：通过对甘肃乌拉尔甘草野生与栽培品内生菌的分离，对甘草同属不同品种内生菌的优势菌种进行初步鉴定，比较二者的生理活性差异。方法：采用NA、PDA培养基对甘草野生与栽培品内生菌进行分离和纯化，并以分离频率比较判断各自优势菌种。结果：野生甘草分离到内生细菌65 株，内生真菌7株，优势菌种为青霉菌属和曲霉属；栽培甘草分离到内生细菌54株，内生真菌6株，优势菌种为青霉菌属；野生与栽培甘草的内生真菌分属于6个属，相同的有5个属，不同的2个属为丝核菌属和木霉属。结论：野生与栽培甘草具有相趋同数量和种类的内生细菌与内生真菌，但二者内生真菌的菌属及优势菌种存在明显差异。
Abstract:
Objective: To compare the differences of biological activity between wild and cultivated glycyrrhiza uralensis through the isolation of endophytes and identification on dominant bacteria from endophytes of different species in the same genera. Method: Endophytes from wild and cultivated GanCao were isolated and purified with NA and PDA base. The dominant bacteria were compared and judged with frequency separation. Result:All 65 strains of endophytic bacteria and seven strains of endophytic fungi were isolated from wild GanCao, the dominant bacteria were penicillium spp and monillales; 54 strains of endophytic bacteria and six strains of endophytic fungi were isolated from cultivated GanCao, the dominant bacteria were penicillium spp; endophytic fungi isolated from wild and cultivated GanCao were in the six genera, there were five same genera, two different genera were rhizoctonia and trichoderma. Conclusion: Wild and cultivated GanCao have almost the same amount and kinds of endo- phytic bacteria and fungi, but there exists remarkable difference in genera and dominant bacteria of endophtic fungi in both.

参考文献/References:

［1］ 丁仁伟，邓毅.药用植物内生菌活性成分及药效学研究进展［J］.西部中医药，2012，25(3)：109-112. ［2］ 魏景超.真菌鉴定手册［M］.上海：上海科技出版社，1979：405-645. ［3］ 饶小莉，沈德龙，李俊，等.甘草内生细菌的分离及拮抗菌株鉴定［J］.微生物学通报，2007，34(4)：700-704.

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备注/Memo

备注/Memo:
收稿日期：2012-04-27 * 基金项目：甘肃省中医药管理局项目(编号 GZK-2010-9) 作者简介：邓毅(1964—)，男，硕士研究生导师，教授，中药学科学术带头人。研究方向：中药学教学、中药性能与配伍应用及中药不良反应的研究。 △通讯作者：张艳萍(1964—)，女，副主任技师。研究方向：心血管疾病的中医药防治。
更新日期/Last Update: 2012-11-15