[1]张亚武,权柯.芍药苷对HepG2肝癌细胞凋亡的诱导作用[J].西部中医药,2016,29(05):23-25.
　ZHANG Yawu,QUAN Ke.Apoptotic Induction Effects of Paeoniflorin on Hepatoma Carcinoma Cells[J].Western Journal of Traditional Chinese Medicine,2016,29(05):23-25.
点击复制

芍药苷对HepG2肝癌细胞凋亡的诱导作用()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年05期
页码:
23-25
栏目:
出版日期:
2016-05-15

文章信息/Info

Title:
Apoptotic Induction Effects of Paeoniflorin on Hepatoma Carcinoma Cells
文章编号:
1004-6852(2016)05-0023-03
作者:
张亚武1，权柯2
1 兰州大学第二医院胆胰外科，甘肃 兰州 730000； 2 甘肃中医药大学
Author(s):
ZHANG Yawu1, QUAN Ke2
1 Biliary Pancreatic Surgery Department of Lanzhou University Second Hospital, Lanzhou 730000, China;2 Gansu University of Traditional Chinese Medicine
关键词:
HepG2肝癌细胞; 凋亡; NF-κB信号通路; 磷酸化; 芍药苷
Keywords:
hepatoma carcinoma cells;  apoptosis;  NF-κB signaling pathway;  phosphorylation;  paeoniflorin
分类号:
R735.7
文献标志码:
A
摘要:
目的：探讨芍药苷(Paeoniflorin)对HepG2肝癌细胞凋亡的诱导作用，并考察其作用机制。方法：用不同浓度芍药苷(0.5，2 mg/mL)对HepG2肝癌细胞进行培养，采用MTT法检测细胞活力，酶标法检测Caspase 3活性，western blot法检测NF-κB信号通路相关蛋白表达。结果：随着给药浓度的增加，芍药苷能逐渐降低HepG2肝癌细胞活力，在48小时抑制率最高；并能提高Caspase 3活性，抑制细胞核内NF-κB p65磷酸化，IκBα磷酸化，从而促进细胞凋亡。结论：芍药苷可能通过NF-κB信号通路诱导HepG2肝癌细胞凋亡，达到抗肿瘤效果。
Abstract:
Objective: To explore apoptotic induction effects of paeoniflorin on hepatoma carcinoma cells (HepG2), and to investigate its mechanism. Methods: HepG2 cells were cultivated with different concentrations of paeoniflorin (0.5, 2 mg/mL), cell viability was detected by MTT method, Caspase 3 activity measured by enzyme linked immunosorbent assay and the expression of NF-κB signaling pathway related protein by western blot method. Results: As the concentrations of the drug increased, paeoniflorin could decrease the viability of HepG2 cells gradually, and the inhibition rate reached the highest within 48 hours; it increased Caspase 3 activity, inhibited the phosphorylation of NF-κB p65 and IκBα, thereby to promote cellular apoptosis. Conclusion: Paeoniflorin could obtain anti-tumor effects by inducing the apoptosis of HepG2 cells via NF-κB signaling pathway.

相似文献/References:

[1]王晓荣,李湧健△,程彬彬.薯蓣皂苷元抗肿瘤作用及其机制研究[J].西部中医药,2014,27(05):140.
　WANG Xiaorong,,et al.Study on Anti-tumor Effects and the Mechanism of Diosgenin[J].Western Journal of Traditional Chinese Medicine,2014,27(05):140.
[2]黄宜生,贾钰华△,糜小英.酸枣仁皂苷A预处理对过氧化氢诱导大鼠心肌细胞凋亡的作用[J].西部中医药,2013,26(07):8.
　HUANG Yisheng,JIA Yuhua,MI Xiaoying.Effects of Jujuboside A Preconditioning on Myocardial Cell Apoptosis Induced by Hydrogen Peroxide in Rat[J].Western Journal of Traditional Chinese Medicine,2013,26(05):8.
[3]李凯,王育良,张传伟.鬼针草治疗阿托品干眼兔模型的实验研究[J].西部中医药,2017,30(02):13.
　LI Kai,WANG Yuliang,ZHANG Chuanwei.Experimental study on Sticktight in Treating Atropine Dry Eye Rabbit Model[J].Western Journal of Traditional Chinese Medicine,2017,30(05):13.
[4]林泳欣,王昌俊△.健脾化瘀方对肝癌HepG-2细胞生理活性的影响[J].西部中医药,2018,31(11):11.
　LIN Yongxin,WANG Changjun.Effects of Spleen-invigorating Stasis-eliminating Prescription on Physiological Activity of HepG-2 Cells in Liver Cancer[J].Western Journal of Traditional Chinese Medicine,2018,31(05):11.
[5]冉广宇.白杨素对结肠癌SW480细胞体内外抑制作用[J].西部中医药,2018,31(11):17.
　RAN Guangyu.The Inhibitory Effects of Chrysin on Colon Cancer SW480 Cells in Vitro and Vivo[J].Western Journal of Traditional Chinese Medicine,2018,31(05):17.
[6]牛媛瑕,张艳,魏丽琼,等.阿魏酸诱导胃癌SGC-7901细胞凋亡及对COX-2、Survivin、XIAP和p53的影响[J].西部中医药,2019,32(01):19.
　NIU Yuanxia,ZHANG Yan,WEI Liqiong,et al.The Inducing Effects of Ferulic Acid on the Apoptosis of Gastric Cancer SGC-7901 Cells and Its Influence on COX-2, Survivin, XIAP and p53[J].Western Journal of Traditional Chinese Medicine,2019,32(05):19.
[7]王盼,谢小青,杨晓娟,等.苦参碱诱导肝癌细胞凋亡的作用机制[J].西部中医药,2025,38(12):6.[doi:10.12174/j.issn.2096-9600.2025.12.02]
　WANG Pan,XIE Xiaoqing,YANG Xiaojuan,et al.The Mechanism of the Apoptosis of Hepatocellular Carcinoma Cells Induced by Matrine[J].Western Journal of Traditional Chinese Medicine,2025,38(05):6.[doi:10.12174/j.issn.2096-9600.2025.12.02]
[8]黄国开,梁淑芳,程彬彬,等.解毒方含药血清对大鼠肝星状细胞HSC-T6增殖和凋亡的影响[J].西部中医药,2026,39(01):1.[doi:10.12174/j.issn.2096-9600.2026.01.01]
　HUANG Guokai,LIANG Shufang,CHENG Binbin,et al.Effects of Drug-containing Serum from Jiedu Formula on the Proliferation and Apoptosis of Rat HSC-T6[J].Western Journal of Traditional Chinese Medicine,2026,39(05):1.[doi:10.12174/j.issn.2096-9600.2026.01.01]
[9]任可可,秦明春.寿胎丸对人早孕滋养细胞增殖与凋亡的影响及相关机制研究[J].西部中医药,2026,39(03):1.[doi:10.12174/j.issn.2096-9600.2026.03.01]
　REN Keke,QIN Mingchun.Effect of Shoutai Pill on Proliferation and Apoptosis of Human Trophoblast Cells in Early Pregnancy and Its Related Mechanism[J].Western Journal of Traditional Chinese Medicine,2026,39(05):1.[doi:10.12174/j.issn.2096-9600.2026.03.01]
[10]贾世艳,仲启明,司瑞花,等.复方蛇龙胶囊对膜性肾病大鼠肾保护作用及对足细胞凋亡的影响[J].西部中医药,2026,39(03):6.[doi:10.12174/j.issn.2096-9600.2026.03.02]
　JIA Shiyan,ZHONG Qiming,SI Ruihua,et al.Renal Protection and Podocyte Apoptosis Modulation by Compound Shelong Capsule in Rats with Membranous Nephropathy[J].Western Journal of Traditional Chinese Medicine,2026,39(05):6.[doi:10.12174/j.issn.2096-9600.2026.03.02]

备注/Memo

备注/Memo:
收稿日期：2015-02-19作者简介：张亚武(1971—)，男，硕士学位，副主任医师。研究方向：消化道肿瘤的诊治。
更新日期/Last Update: 2016-05-15