[1]王文鹏,冯玉康,郝鹏彬△,等.甘草提取物HPLC指纹图谱及质量评价研究[J].西部中医药,2017,30(11):52-55.
　WANG Wenpeng,FENG Yukang,HAO Pengbin,et al.Study on HPLC Fingerprint and Quality Evaluation of Licorice Extract[J].Western Journal of Traditional Chinese Medicine,2017,30(11):52-55.
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甘草提取物HPLC指纹图谱及质量评价研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年11期
页码:
52-55
栏目:
出版日期:
2017-11-15

文章信息/Info

Title:
Study on HPLC Fingerprint and Quality Evaluation of Licorice Extract
文章编号:
1004-6852(2017)11-0052-04
作者:
王文鹏1，冯玉康1，郝鹏彬1△，丁艳谱1，周永妍1，张浩军1，孙胜斌1，姜国志2
1 神威药业集团有限公司，河北 石家庄 051430； 2 中药注射剂新药技术开发国家地方联合工程实验室
Author(s):
WANG Wenpeng1, FENG Yukang1, HAO Pengbin1△, DING Yanpu1, ZHOU Yongyan1, ZHANG Haojun1, SUN Shengbin1, JIANG Guozhi2
1 China Shineway Pharmaceutical Group Ltd., Shijiazhuang 051430, China; 2 State-local Joint Engineering Laboratory of New Drugs Development Technology for TCM Injection
关键词:
甘草; 指纹图谱; 甘草苷; 甘草酸
Keywords:
licorice;  fingerprint;  liquiritin;  glycyrrhizic acid
分类号:
R284.2
摘要:
目的：结合甘草苷和甘草酸的含量测定建立甘草提取物的HPLC指纹图谱方法。方法：采用高效液相色谱法，选用Diamonsil-C18 ODS(4.6 mm×250 mm，5 μm)色谱柱，乙腈-0.1%磷酸为流动相梯度洗脱，流速0.8 mL/min，检测波长237 nm。结果：建立甘草提取物的HPLC指纹图谱，确定13个共有峰，方法学验证符合指纹图谱相关要求。结论：指纹图谱结合有效成分含量测定的方法可用于对甘草提取物的整体质量评价，对其质量控制具有指导意义。
Abstract:
Objective: To establish HPLC fingerprint method of licorice extract by taking the contents of glycyrrhizic acid and liquiritin as the indexes. Methods: HPLC was used, Diamonsil-C18 ODS chromatographic column (4.6 mm×250 mm, 5 μm) was chosen, acetonitrile-0.1% phosphoric acid was taken as mobile phase in the mode of gradient elution, flow velocity was 0.8 mL/min and detection wavelength was 237 nm. Results: HPLC fingerprint of licorice was established, 13 common peaks were confirmed, and the methodology verification met the related requirements of fingerprint. Conclusion: Fingerprint combined with content determination of active ingredients could be used for the evaluation of total body mass of licorice extract, which is meaningful to quality control of licorice extract.

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备注/Memo

备注/Memo:
收稿日期：2017-04-20 *基金项目：国家中医药管理局中医药科学技术研究专项课 题(编号2013ZX07)。 作者简介：王文鹏(1985—)，男，工程师。研究方向：中药提取物质量标准及工艺研究。 △通讯作者：郝鹏彬(1986—)，男，工程师。研究方向：中成药工艺与质量标准研究。
更新日期/Last Update: 2017-11-15