[1]邱泉,覃聪慧,黄秋燕,等.神农蛇药酒的质量标准研究[J].西部中医药,2019,32(06):21-27.
　QIU Quan,QIN Conghui,HUANG Qiuyan,et al.Quality Standard of ShenNong Snake Liquor[J].Western Journal of Traditional Chinese Medicine,2019,32(06):21-27.
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神农蛇药酒的质量标准研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年06期
页码:
21-27
栏目:
出版日期:
2019-06-15

文章信息/Info

Title:
Quality Standard of ShenNong Snake Liquor
文章编号:
1004-6852(2019)06-0021-07
作者:
邱泉，覃聪慧，黄秋燕，梁平
梧州市中西医结合医院，广西 梧州 543002
Author(s):
QIU Quan, QIN Conghui, HUANG Qiuyan, LIANG Ping
Wuzhou City Hospital of Traditional Chinese Medicine and Western Medicine, Wuzhou 543002, China
关键词:
蛇药酒; 质量标准; 薄层色谱法; 高效液相色谱法
Keywords:
snake liquor;  quality standard;  TLC;  HPLC
分类号:
R932
文献标志码:
A
摘要:
目的：研究神农蛇药酒的质量标准。方法：采用薄层色谱法对神农蛇药酒中半边莲、东风菜、红背丝绸、小叶三点金、通城虎进行定性鉴别；采用高效液相色谱法对蛇药酒中通城虎的主要成分马兜铃酸A进行含量测定。采用Accucore C18色谱柱(3×100 mm，2.6 μm)，以0.1%磷酸溶液为流动相A，以乙腈为流动相B，进行梯度洗脱，流速为0.3 mL/min，检测波长250 nm，室温下进样量10 μL。结果：薄层色谱对处方中半边莲、红背丝绸、通城虎的薄层鉴别方法重现性好、专属性强，定性鉴别色谱特征明显，易于区别。马兜铃酸A在1.009 6～100.96 mg/L线性关系良好(r＝0.999 9)，回归方程Y=0.235 1X+0.0528，平均回收率为103.50%，RSD=1.90%。结论：建立的质量标准测定方法简便、准确，灵敏度高，重复性好，可用于神农蛇药酒的质量控制。
Abstract:
Objective: To study quality standard of ShenNong snake liquor. Methods: Banbianlian (Lobelia chinensis Lour), Dongfengcai [Doellingeria marchandii(Levl.) Ling], Hongbei sichou [Cissus assamica(Laws.)Craib], Xiaoye sandianjin[Desmodium microphyllum(Thunb.)DC]and Tongchenghu(Aristoloehia fordiana Hemsl) in ShenNong snake liquor were qualitatively identified using TLC; the contents of aristolochic acid A, the main ingredients of Tongchenghu in snake liquor, were determined using HPLC. Accucore C18 chromatographic column(3×100 mm, 2.6 μm) was used, 0.1% phosphoric acid solution was chosen as mobile phase A, acetonitrile as mobile phase B, gradient elution, current velocity: 0.3 mL/min, detection wavelength 250 nm, sample size was 10 μL under room temperature. Results: Thin-layer chromatography of Banbianlian, Hongbei sichou and Tongchenghu was reproducible and specific, qualitative identification chromatography were featured obviously, and easy to distinguish. Aristolochic acid A showed good linear relationship in the range between 1.009 6 and 100.96 mg/L(r=0.999 9), regression equation Y=0.235 1X+0.052 8, average recovery rate was 103.50%, RSD=1.90%. Conclusion: The determination method is simple, accurate, sensitive and reproducible, and it could used for quality control of ShenNong snake liquor.

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备注/Memo

备注/Memo:
收稿日期：2018-11-08 *基金项目：梧州市科学研究与技术开发计划项目(编号 201402212)。 作者简介：邱泉(1964—)，女，副主任药师。研究方向：医院药学管理及中药制剂研发。
更新日期/Last Update: 2019-06-15