[1]郭凯凯,赵艺蔓,陈晓宇,等.基于网络药理学方法研究“黄芪-人参”药对治疗胃癌的作用机制[J].西部中医药,2022,35(11):40-46.[doi:10.12174/j.issn.2096-9600.2022.11.08]
　GUO Kaikai,ZHAO Yiman,CHEN Xiaoyu,et al.The Mechanism of "Huangqi-ginseng" Drug Pair in the Treatment of Gastric Cancer Based on Network Pharmacology[J].Western Journal of Traditional Chinese Medicine,2022,35(11):40-46.[doi:10.12174/j.issn.2096-9600.2022.11.08]
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基于网络药理学方法研究“黄芪-人参”药对治疗胃癌的作用机制
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
35
期数:
2022年11期
页码:
40-46
栏目:
出版日期:
2022-11-15

文章信息/Info

Title:
The Mechanism of "Huangqi-ginseng" Drug Pair in the Treatment of Gastric Cancer Based on Network Pharmacology
作者:
郭凯凯, 赵艺蔓, 陈晓宇, 凌慧, 李洲强, 郭东霖, 张颖, 侯恩存
广西中医药大学附属瑞康医院，广西 南宁 530011
Author(s):
GUO Kaikai, ZHAO Yiman, CHEN Xiaoyu, LING Hui, LI Zhouqiang, GUO Donglin, ZHANG Ying, HOU Encun
Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, China
关键词:
中药; 网络药理学; 胃癌; 机制; 黄芪-人参
Keywords:
herbs; network pharmacology; gastric cancer; mechanism; ginseng
分类号:
R962
DOI:
10.12174/j.issn.2096-9600.2022.11.08
文献标志码:
A
摘要:
目的应用网络药理学方法探讨“黄芪-人参”药对治疗胃癌的作用机制。 方法依托中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform，TCMSP)检索“黄芪-人参”中药化学成分、作用靶点，并通过人类基因数据库（the human gene database,GeneCards）、比较毒物基因组学数据库（CTD）得到胃癌相关基因，探寻“黄芪-人参”药对中与胃癌相关的作用靶点，建立“化合物-靶点”网络图；依据STRING平台构建蛋白-蛋白相互作用网络(protein-protein interactions，PPI)，筛选出核心靶点，提交到生物信息注释数据库（DAVID）进行基因富集分析，获得基因本体论功能富集分析（gene ontology，GO）及KEGG富集分析（kyoto encyclopedia of genes and genomes，KEGG）注释结果。 结果依据筛选条件［口服生物利用度（oral bioavailability，OB）≥30%、药物相似性（drug-likeness，DL）≥0.18）］共得到41个活性成分，其中黄芪20个，人参22个，药物潜在靶点384个。2个疾病数据库中共收集到200个疾病靶点。通过韦恩图筛选出黄芪-人参作用于胃癌的22个关键靶点，通过degree值做PPI网络进行功能分析，共选出5个关键靶点，主要涉及丝氨酸/苏氨酸蛋白激酶、丝裂原活化蛋白激酶8、胱天蛋白酶3、前列腺素内过氧化物合成酶2、胱天蛋白酶9等。GO生物过程分析以P＜0.01通过靶基因筛选出排名靠前的条目，主要包括血管平滑肌增殖、凋亡过程、对细胞因子的反应、染色质结合、DNA结合、蛋白质同聚活性等。KEGG富集结果筛选出与胃癌相关的前20条信号通路，主要涉及神经营养蛋白信号通路、Toll样受体信号通路、肿瘤坏死因子等。 结论“黄芪-人参”药对通过调控致癌物质代谢、免疫耐药、炎症反应，对癌症的发生、转移及耐药发挥防治胃癌的作用。
Abstract:
ObjectiveTo explore the mechanism of the compatibility of "Huangqi-ginseng" in the treatment of gastric cancer by applying network pharmacology. MethodsChemical ingredients and the targets of "Huangqi-ginseng" were searched from TCMSP, and the related genes of gastric cancer were gained via GeneCards and CTD databases, the targets associated with gastric cancer were surveyed in the drug pair, to establish the network planning "compound-targets"; PPI was constructed according to STRING platform, the core targets were screened out and submitted to DAVID database to conduct gene enrichment anaysis, obstain GO biological function and KEGG signaling pathway annotation results. ResultsIn light of the screening conditions [OB≥30%, DL≥0.18], 41 active ingredients were gained, among them, 20 ones were about Huangqi, 22 about ginseng and 384 potential herbal targets. All 200 disease targets were collected from two disease databases. Five key targets, mainly involving AKT1, MAPK8, CASP3, PTJS2, CASP9 and others,were chosen after conducting functional analysis of PPI network by degree values. In GO biological process analysis, P<0.01 was used to screen the top items by target genes, mainly covering the process of vascular smooth muscle proliferation and apoptosis, response to cytokines, chromatin binding, DNA binding, protein hormerization activity and others. The top 20 signaling pathways were screened out in KEGG enrichment results, involving neurotrophin signaling pathway, Toll receptor signaling pathway, TNF and others. ConclusionCompatibility of "Huangqi-ginseng" could develop preventive and therapeutic effects by regulating the metabolism of cancerogenic substance, immune resistance, inflammatory reaction, the occurrence, the metastasis and drug resistance of cancer.

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备注/Memo

备注/Memo:
郭凯凯（1994—），男，硕士学位，医师。研究方向：恶性肿瘤的中西医结合防治。国家科学研究基金（YWJKJJHKYJJ-F3208D）；北京医学奖励基金（YJHYXKYJJ-359）。
更新日期/Last Update: 2022-11-15