[1]黄晓燕,邹孟龙,陈雅璐,等.基于生物信息学筛选铁死亡参与溃疡性结肠炎的核心基因及中药治疗预测[J].西部中医药,2023,36(12):50-54.[doi:10.12174/j.issn.2096-9600.2023.12.12]
　HUANG Xiaoyan,ZOU Menglong,CHEN Yalu,et al.Bioinformatics-based Screening of Core Genes of Ferroptosis Involved in Ulcerative Colitis and the Prediction of Chinese Medicine Therapy[J].Western Journal of Traditional Chinese Medicine,2023,36(12):50-54.[doi:10.12174/j.issn.2096-9600.2023.12.12]
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基于生物信息学筛选铁死亡参与溃疡性结肠炎的核心基因及中药治疗预测
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年12期
页码:
50-54
栏目:
出版日期:
2023-12-15

文章信息/Info

Title:
Bioinformatics-based Screening of Core Genes of Ferroptosis Involved in Ulcerative Colitis and the Prediction of Chinese Medicine Therapy
作者:
黄晓燕1, 邹孟龙2, 陈雅璐1, 宁芯2, 陈柏辉2, 梁巧俐2
1.广西中医药大学第一附属医院，广西 南宁 530023
2.广西中医药大学研究生学院，广西 南宁 530001
Author(s):
HUANG Xiaoyan1, ZOU Menglong2, CHEN Yalu1, NING Xin2, CHEN Baihui2, LIANG Qiaoli2
1.The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
2.Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China
关键词:
结肠炎; 溃疡性; 生物信息学; GEO数据库; 铁死亡; 中药
Keywords:
colitis;  ulcerative; bioinformatics; GEO database; ferroptosis; Chinese medicine
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2023.12.12
文献标志码:
A
摘要:
目的基于生物信息学筛选铁死亡参与溃疡性结肠炎（ulcerative colitis，UC）的核心基因，并预测中药治疗。 方法利用基因综合表达（gene expression omnibus，GEO）数据库筛选UC差异表达基因；通过常用的中文及英文数据库检索铁死亡相关基因，然后绘制UC差异基因与铁死亡相关基因的Venn图，取得交集基因。通过STRING数据库对交集基因进行蛋白相互作用分析（protein-protein interaction，PPI）；借助Coremine数据库预测潜在治疗中药，筛选出具有调控细胞铁死亡防治UC效用的中药，最后对得到的中药进行归纳。 结果共获得UC差异表达基因1355个，其中上调基因796个，下调基因559个；获得60个铁死亡相关基因；与铁死亡相关的UC差异表达基因共8个，分别是CD44、DPP4、LPCAT3、PTGS2、SLC7A11、ABCC1、HMOX1、ACSF2，其中CD44在PPI网络中度值最高；预测得到的中药多归脾经、胃经，功效多属于清热类、补虚类、活血类，其中黄芩、三百草、牡丹皮、人参、生姜、干姜、五味子、丹参、木香、三七等中处于核心地位。 结论黄芩、人参、丹参、三七等中药可能通过干预CD44蛋白的表达，调控细胞铁死亡，从而发挥治疗UC作用。
Abstract:
ObjectiveTo screen the core genes of ferroptosis participated in ulcerative colitis (UC) based on bioinformatics and predict the treatment of Chinese medicine. MethodsGEO database was utilized to screen UC differentially expressed genes; the commonly used Chinese and English databases were applied to retrieve ferroptosis-related genes, and then the intersection genes were obtained by drawing the Venn diagram of UC differential genes and ferroptosis-related genes. PPI analysis of the intersection genes were performed through STRING databse; the potential Chinese medicines were predicted via Coremine database, to screen out the herbs with the efficacy in preventing and treating UC by regulating cellular iron death, and finally to classify the obtained Chinese medicines. ResultsThere were 1355 UC differential genes, including 796 up-regulated genes and 559 down-regulated genes; 60 ferroptosis-related genes were obtained; and eight UC differential genes related to ferroptosis contained CD44, DPP4, LPCAT3, PTGS2, SLC7A11, ABCC1, HMOX1 and ACSF2, among them, CD44 had the highest moderate value in PPI network; Chinese medicine obtained in the prediction mostly entered the meridians of spleen and stomach, with the efficacy of clearing heat, tonifying and promoting blood circulation mostly, Huangqin (Scutellariae radix), Sanbaicao (Herba centaurii tendonis), Mudanpi (Moutan cortex), ginseng, ginger, dried ginger, Wuweizi (Schisandrae Chinensis fructus), Danshen (Salviae miltiorrhizae radix et rhizoma), Muxiang (Aucklandiae radix), Sanqi (Notoginseng radix et rhizoma) are in the core position. ConclusionChinese medicines including Huangqi, ginseng, Danshen and Sanqi could play a therapeutic role in the treatment of UC possibly through intervening the expression of CD44 protein and regulating cellular iron death.

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备注/Memo

备注/Memo:
黄晓燕（1979—），女，博士学位，硕士研究生导师，主任医师。研究方向：脾胃病的中医药防治研究。国家自然科学基金（81460714）；广西自然科学基金（2020GXNSFAA238009）；广西科技重大专项（桂科AA17202031）；广西中医药大学研究生教育创新计划资助项目（YCSY2020034）基金项目:。
更新日期/Last Update: 2023-12-15