[1]王盼,谢小青,杨晓娟,等.苦参碱诱导肝癌细胞凋亡的作用机制[J].西部中医药,2025,38(12):6-10.[doi:10.12174/j.issn.2096-9600.2025.12.02]
　WANG Pan,XIE Xiaoqing,YANG Xiaojuan,et al.The Mechanism of the Apoptosis of Hepatocellular Carcinoma Cells Induced by Matrine[J].Western Journal of Traditional Chinese Medicine,2025,38(12):6-10.[doi:10.12174/j.issn.2096-9600.2025.12.02]
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苦参碱诱导肝癌细胞凋亡的作用机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年12期
页码:
6-10
栏目:
基础研究
出版日期:
2025-12-15

文章信息/Info

Title:
The Mechanism of the Apoptosis of Hepatocellular Carcinoma Cells Induced by Matrine
作者:
王盼1, 谢小青1, 杨晓娟2, 梁昭君1, 何昱静1, 卢利霞1, 王俊科1, 李斌1, 张久聪1, 于晓辉1, 郑英1
1.中国人民解放军联勤保障部队第九四〇医院，甘肃 兰州 730050
2.甘肃省第三人民医院，甘肃 兰州 730000
Author(s):
WANG Pan1, XIE Xiaoqing1, YANG Xiaojuan2, LIANG Zhaojun1, HE Yujing1, LU Lixia1, WANG Junke1, LI Bin1, ZHANG Jiucong1, YU Xiaohui1, ZHENG Ying1
1.Hospital 940 of PLA Joint Logistics Support Force, Lanzhou 730050, China
2.the Third People’s Hospital of Gansu Province, Lanzhou 730000, China
关键词:
苦参碱; 肝癌细胞; 加丝裂原活化蛋白激酶p38信号通路; c-Jun氨基末端激酶信号通路; 凋亡
Keywords:
matrine; hepatocellular carcinoma cells; P38MAPK; JNK; apoptosis
分类号:
R285.5
DOI:
10.12174/j.issn.2096-9600.2025.12.02
文献标志码:
A
摘要:
目的基于P38MAPK和JNK信号通路探讨苦参碱诱导肝癌细胞凋亡的机制。 方法采用膜联蛋白V-碘化丙啶双染法染色HepG2和BEL-7404细胞，将4个浓度的苦参碱作用后，用流式细胞仪检测各组细胞的凋亡能力，选择出苦参碱促进凋亡的最宜浓度，将该浓度的苦参碱作用于HepG2和BEL-7404细胞作为苦参碱组，不加苦参碱的作为空白对照组，加丝裂原活化蛋白激酶p38（mitogen-activated protein kinase p38，P38MAPK）抑制剂组和c-Jun氨基末端激酶（c-Jun N-terminal kinase，JNK）抑制剂组为阳性对照组。Western Blot检测各组细胞中P38MAPK、磷酸化丝裂原活化蛋白激酶（phosphorylated mitogen-activated protein kinase p38，p-P38MAPK）、JNK、B淋巴细胞瘤2（B-cell lymphoma-2，Bcl-2）和B淋巴细胞瘤2相关蛋白（BCL-2-associated X protein，Bax）。 结果苦参碱促进HepG2和BEL-7404细胞凋亡的最宜浓度为3 mg/mL，实验组（3 mg/mL 苦参碱）与空白对照组和阳性对照组比较，P38MAPK和JNK蛋白的表达无差异（P＞0.05），但p-P38MAPK和p-JNK、Bax及Bcl-2的表达有差异（P＜0.05）。 结论苦参碱通过上调P38MAPK和JNK信号通路促进肝癌细胞凋亡，进而发挥抑制肝癌细胞增殖的作用。
Abstract:
ObjectiveTo discuss the mechanism of inducing the apoptosis of hepatocellular carcinoma cells with matrine based on P38MAPK and JNK signaling pathway. MethodsHepG2 and BEL-7404 cells were stained by annexin V-propidium iodide double staining method of membrane-associated protein, the apoptotic ability of each group of cells was detected by flow cytometry after the action of four concentrations of matrine, for the selection of optimal concentration of matrine to promote the apoptosis, HepG2 and BEL-7404 cells were chosen as matrine group after applying the concentrations of matrine to them, blank control group didn’t add matrine, P38MAPK inhibitor group and JNK inhibitor group were taken as positive control groups. Western Blot was applied to detect the expressions of P38MAPK, p-P38MAPK, JNK, Bcl-2 and Bax in the cells of different groups. ResultsThe optimal concentrations of matrine to promote the apoptosis of HepG2 and BEL-7404 cells was 3 mg/mL, when the experiment group (3 mg/mL matrine) was compared with blank control group and positive control group, there was no difference in the expressions of P38MAPK and JNK (P>0.05), while there was difference in the expressions of p-P38MAPK and p-JNK, Bax and Bcl-2 (P<0.05). ConclusionMatrine could promote the apop-tosis of hepatocellular carcinoma cells by upregulating P38MAPK and JNK signaling pathway, thereby exerting the effects of inhibiting the proliferation of hepatocellular carcinoma cells.

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备注/Memo

备注/Memo:
国家自然科学基金（81874358，82174029）。 甘肃省科技厅重点研发计划-社发类资助项目（20YF8FA099，22YF7FA105）；甘肃省科技计划项目-省青年科技基金计划（21JR7RA012）；兰州市人才创新项目资助（2020-RC-111）；兰州市科技计划项目（2024-9-166）。刘雨溪（1993—），女，博士学位，助理研究员。研究方向：中药药理。；王盼（1994—），男，硕士学位，主治医师。研究方向：消化系统肿瘤的基础与临床研究。
更新日期/Last Update: 2025-12-15