[1]苏韫,刘永琦,颜春鲁,等.黄芪多糖对脑缺血再灌注大鼠脑组织Bcl-2、Bax蛋白及NO含量的影响[J].西部中医药,2016,29(03):10-13.
　SU Yun,LIU Yongqi,YAN Chunlu,et al.Influence of Astragalus Polysaccharides on the Contents of Bcl-2, Bax Protein and NO in the Brain Tissue of the Rats with Cerebral Ischemia Reperfusion[J].Western Journal of Traditional Chinese Medicine,2016,29(03):10-13.
点击复制

黄芪多糖对脑缺血再灌注大鼠脑组织Bcl-2、Bax蛋白及NO含量的影响()
分享到：

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年03期
页码:
10-13
栏目:
出版日期:
2016-03-15

文章信息/Info

Title:
Influence of Astragalus Polysaccharides on the Contents of Bcl-2, Bax Protein and NO in the Brain Tissue of the Rats with Cerebral Ischemia Reperfusion
文章编号:
1004-6852(2016)03-0010-04
作者:
苏韫1，刘永琦1，颜春鲁1，薛娜1，伍志伟1，聂蕾1，王琰2
1 甘肃中医药大学，甘肃 兰州 730020； 2 甘肃省妇幼保健院
Author(s):
SU Yun1, LIU Yongqi1, YAN Chunlu1, XUE Na1, WU Zhiwei1, NIE Lei1, WANG Yan2
1 Gansu University of Traditional Chinese Medicine, Lanzhou 730020, China;2 Gansu Provincial Maternity and Child-care Hospital
关键词:
脑缺血再灌注; Bcl-2; Bax; NO; 黄芪多糖
Keywords:
cerebral ischemia reperfusion;  Bcl-2;  Bax;  NO;  astragalus polysaccharides
分类号:
R286
文献标志码:
A
摘要:
目的：探讨黄芪多糖对大鼠脑缺血再灌注损伤后脑组织Bcl-2、Bax蛋白及NO含量的影响。方法：将SPF级Wistar大鼠72只分为假手术组、模型组、阳性对照组及黄芪多糖高、中、低剂量组，采用改良的线栓法制作大鼠脑缺血再灌注模型，各药物组分别给予相应药物15天后采用免疫组化法检测Bax、Bcl-2蛋白含量的表达，采用硝酸盐还原酶法检测NO蛋白含量的表达。结果：1)Bcl-2、Bax的含量模型组较假手术组表达明显增加；与模型组比较，Bcl-2表达黄芪多糖高、中剂量组增加(P＜0.05)；Bax含量黄芪多糖高剂量组表达显著减少(P＜0.05)。与假手术组比较,模型组Bcl-2/Bax比值明显降低，阳性对照组与黄芪多糖各剂量组比较比值升高。2）与假手术组比较，模型组大鼠脑组织中NO的水平明显升高(P＜0.05)，与模型组比较，黄芪多糖高剂量组中NO含量明显降低(P＜0.05)。结论：黄芪多糖可增加凋亡抑制基因Bcl-2蛋白和降低凋亡促进基因Bax蛋白的表达，通过增加Bcl-2/Bax比例，降低NO含量，减少细胞凋亡的凋亡程度。
Abstract:
Objective: To explore the influence of astragalus polysaccharides on the contents of Bcl-2, Bax protein and NO in the brain tissue of the rats after cerebral ischemia reperfusion. Methods: Seventy-two SPF rats were randomized into sham operation group, the model group, positive control group, high, moderate and low doses groups of astragalus polysaccharides, the rat model was prepared by reformed middle cerebral artery occlusion, the expressions of Bax protein and Bcl-2 protein were detected with immunohistochemical method after different medication groups were administered with the corresponding herbs respectively, the expressions of NO protein was detected by nitrate reductase method. Results: 1) The contents of Bcl-2 and Bax in the model groups increased notably compared with sham operation group; compared with the model group, the expressions of Bcl-2 increased in high and moderate doses groups of astragalus polysaccharides (P＜0.05); the contents of Bax protein decreased significantly in high dose group of astragalus polysaccharides (P＜0.05). Compared with sham operation group, the ratio of Bcl-2/Bax lowered notably in the model group, it rose when positive control group was compared with different doses groups of astragalus polysaccharides. 2) Compared with sham operation group, the level of NO raised remarkably in the model group (P＜0.05), compared with the model group, the contents of NO decreased evidently in the high dose group of astragalus polysaccharides (P＜0.05). Conclusion: Astragalus polysaccharides could improve the expressions of Bcl-2 and Bax protein; it could reduce the degrees of cellular apoptosis by increasing the ratio of Bcl-2/Bax, and decreasing the contents of NO.

相似文献/References:

[1]邢涛,董林△,魏国俊,等.骨刺消巴布剂对大鼠急性软组织损伤细胞凋亡的影响*[J].西部中医药,2013,26(12):17.
　XING Tao,DONG Lin,WEI Guojun,et al.Effects of GuCiXiao Cataplasm on Cellular Apoptosis of Rat with Acute Soft Tissue Injury[J].Western Journal of Traditional Chinese Medicine,2013,26(03):17.
[2]赵成国,秦书芝,毛晶磊,等.瑶宝克癌灵胶囊抗肿瘤作用机制研究[J].西部中医药,2014,27(05):7.
　ZHAO Chengguo,QIN Shuzhi,MAO Jinglei,et al.Investigation on Anti-tumor Mechanism of YaoBao KeAiLing Capsules[J].Western Journal of Traditional Chinese Medicine,2014,27(03):7.
[3]王翠兰.乳奥康对乳腺癌细胞株MCF-7 增殖及BCL-2 表达的影响[J].西部中医药,2013,26(01):4.
　WANG Cuilan.Effects of RuA oKang on Expression of BCL-2 and Proliferation of Mammary Cancer Cell Line MCF-7[J].Western Journal of Traditional Chinese Medicine,2013,26(03):4.
[4]严亚锋,白海侠.消栓通络胶囊对急性脑缺血再灌注大鼠脑组织TNF-α和IL-1β水平的影响[J].西部中医药,2013,26(09):23.
　YAN Yafeng,BAI Haixia.Effects of XiaoShuangTongLuo Capsule on TNF-α and IL-1β in Cerebral Tissue of Rats with Acute Cerebral Ischemia-reperfusion Injury[J].Western Journal of Traditional Chinese Medicine,2013,26(03):23.
[5]梁涢.四物汤对脑缺血再灌注小鼠海马结构及脑组织SOD和GSH-PX活力的影响[J].西部中医药,2017,30(02):10.
　The Effects of SiWu Decoction on Hippocampus and Activity of Brain Tissue SOD and GSH-PX in Mice with Cerebral Ischemia-reperfusion.The Effects of SiWu Decoction on Hippocampus and Activity of Brain Tissue SOD and GSH-PX in Mice with Cerebral Ischemia-reperfusion[J].Western Journal of Traditional Chinese Medicine,2017,30(03):10.
[6]刘天易.芍药苷对大鼠脑缺血再灌注损伤细胞凋亡的影响及其作用机制[J].西部中医药,2016,29(05):8.
　LIU Tianyi.The Effects of Peoniflorin on Cellular Apoptosis of the Rats with Cerebral Ishchemia Reperfusion Injury and Its Mechanism[J].Western Journal of Traditional Chinese Medicine,2016,29(03):8.
[7]甘雨,马进△,袁媛,等.线栓法制备SD大鼠局灶性脑缺血再灌注模型的实践与评价[J].西部中医药,2018,31(07):12.
　GAN Yu,MA Jin,YUAN Yuan,et al.Practice and Evaluation of Focal Cerebral Ischemia Reperfusion Rat Model Prepared by Middle Cerebral Artery Occlusion[J].Western Journal of Traditional Chinese Medicine,2018,31(03):12.
[8]段洪超,王庆超,张杰△.丹参注射液对兔软骨细胞增殖与凋亡的影响[J].西部中医药,2018,31(07):22.
　DUAN Hongchao,WANG Qingchao,ZHANG Jie.Effects of DanShen Injection on Apoptosis and Proliferation of Rabbit Chondrocytes[J].Western Journal of Traditional Chinese Medicine,2018,31(03):22.
[9]胡玥,邹玺,王瑞平,等.益气健脾解毒方对胃癌细胞的抑制作用及其机制研究[J].西部中医药,2019,32(01):14.
　HU Yue,ZOU Xi,WANG Ruiping,et al.Study on the Inhibitory Effects of Replenishing-Qi Invigorating-spleen Detoxifying Prescription on Gastric Cancer Cells and Its Mechanism[J].Western Journal of Traditional Chinese Medicine,2019,32(03):14.
[10]吴建军,丁高恒,殷晓春,等.敦煌遗方“神仙粥”对AD模型大鼠学习记忆能力及海马组织Bcl-2、Bax基因表达的影响[J].西部中医药,2019,32(04):19.
　WU Jianjun,DING Gaoheng,YIN Xiaochun,et al.Effects of DunHuang YiFang "ShenXian Zhou" on Learning and Memory Ability, the Expressions of Bcl-2 and Bax Gene in the Hippocampus of AD Rat Model[J].Western Journal of Traditional Chinese Medicine,2019,32(03):19.

备注/Memo

备注/Memo:
*基金项目：甘肃省教育厅第二批科研项目(编号1006B-08)；甘肃中医药大学校内中青年基金(编号BH2010-076)。作者简介：苏韫(1977—)，女，硕士学位，副教授。研究方向：中医药免疫学研究。
更新日期/Last Update: 2016-03-15