[1]李慧,田耀洲,陆敏 夏军权△.养阴和胃方对糖尿病胃轻瘫大鼠SCF/c-kit系统的影响[J].西部中医药,2017,30(12):9-12.
 LI Hui,TIAN Yaozhou,LU Min,et al.Effects of Nourishing-Yin Harmonizing-Stomach Prescription on SCF/c-kit System of the Rats with Diabetic Gastroparesis[J].Western Journal of Traditional Chinese Medicine,2017,30(12):9-12.
点击复制

养阴和胃方对糖尿病胃轻瘫大鼠SCF/c-kit系统的影响
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年12期
页码:
9-12
栏目:
方药·药理研究
出版日期:
2017-12-15

文章信息/Info

Title:
Effects of Nourishing-Yin Harmonizing-Stomach Prescription on SCF/c-kit System of the Rats with Diabetic Gastroparesis
文章编号:
1004-6852(2017)12-0009-04
作者:
李慧12田耀洲12陆敏12 夏军权12△
1 南京中医药大学附属中西医结合医院,江苏 南京 210028; 2 江苏省中医药研究院
Author(s):
LI Hui12 TIAN Yaozhou12 LU Min12 XIA Junquan12△
1 Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China; 2 Jiangsu Province Academy of Traditional Chinese Medicine
关键词:
糖尿病胃轻瘫SCF/c-kitCajal间质细胞养阴和胃方
Keywords:
diabetic gastroparesis SCF/c-kit Cajal interstitial cells Yin-nourishing harmonizing-stomach prescription
分类号:
R285.5
文献标志码:
A
摘要:
目的:观察养阴和胃方对糖尿病胃轻瘫大鼠的疗效及其对SCF/c-kit系统的影响。方法:将40只SD大鼠随机均分为正常对照组,模型对照组,养阴和胃方低、高剂量组,西药组,每组8只。采用腹腔注射注射用链脲佐菌素(STZ)建立糖尿病胃轻瘫大鼠模型,养阴和胃方低、高剂量组和西药组分别用低、高剂量养阴和胃方和吗丁啉混悬液灌胃。酚红灌胃实验测定胃排空率,Western Blot检测胃窦c-kit蛋白和清干细胞因子(stem cell factor,SCF)蛋白的表达,ELISA测定血清SCF含量。结果:与模型对照组相比,各给药组大鼠的胃排空率、c-kit蛋白、SCF蛋白和血清SCF含量均明显增高,差异有统计学意义(P<0.05),且随着中药浓度的增高,胃排空率、c-kit蛋白、SCF蛋白和血清SCF含量增高更加明显,差异有统计学意义(P<0.05)。结论:中药养阴和胃方可上调SCF/c-kit系统的表达,从而逆转DGP胃排空障碍。
Abstract:
Objective: To observe the clinical effects of Yin-nourishing harmonizing-stomach prescription in treating the rats with diabetic gastroparesis (DGP) and investigate its influence on SCF/c-kit system. Methods: Forty SD rats were allocated to normal control group, model control group, western medicine group, low and high doses groups of Yin-nourishing harmonizing-stomach prescription randomly, eight rats each group. DGP rat model was established by intraperitoneal injection of STZ, low, high doses groups of Yin-nourishing harmonizing-stomach prescription and western medicine group were drenched with low, high doses of Yin-nourishing harmonizing-stomach prescription and motilium suspension. Gastric emptying rate was determined by phenol red gavage experiment, the expressions of c-kit and SCF proteins in gastric tissues were analyzed by Western blot, and the levels of SCF in the blood were detected by ELISA. Results: Compared with model control group, gastric emptying rate, the contents of c-kit protein, SCF protein and SCF in the serum raised obviously in the rats of different medication groups (P<0.05), as the concentrations of herbs increased, gastric emptying rate, the contents of c-kit protein, SCF protein and SCF in the serum raised obviously, and the difference had statistical meaning(P<0.05). Conclusion: Yin-nourishing harmonizing-stomach prescription could up regulate the expressions of SCF/c-kit system, therefore to reverse gastric emptying disorder of DGP.

相似文献/References:

[1]赵静,张建.半夏泻心汤治疗糖尿病胃轻瘫临床观察[J].西部中医药,2014,27(11):104.
[2]冯伟.多潘立酮联合香砂养胃片治疗糖尿病胃轻瘫54例[J].西部中医药,2015,28(06):114.[doi:2015/6/16 0:00:00]
[3]沈雁鹏.雷火神针联合半夏泻心汤加减治疗糖尿病胃轻瘫的临床观察[J].西部中医药,2016,29(05):92.
 SHEN Yanpeng.Clinical Observation on Thunder-fire Miraculous Needle Combined with Modified BanXia XieXin Tang in Treating Diabetic Gastroparesis[J].Western Journal of Traditional Chinese Medicine,2016,29(12):92.
[4]郭晓颖,丁文君△,指导:廖志峰.廖志峰主任医师从脾论治糖尿病胃轻瘫[J].西部中医药,2017,30(04):33.
 GUO Xiaoying,DING Wenjun Director: LIAO Zhifeng.Chief Physician LIAO Zhifeng′s Treatment for Diabetic Gastroparesis Based on Spleen Governing Muscles[J].Western Journal of Traditional Chinese Medicine,2017,30(12):33.
[5]李吉庆,林道斌,张永杰.四君消痞汤对糖尿病胃轻瘫大鼠胃肠动力及胃肠激素的影响[J].西部中医药,2022,35(05):17.[doi:10.12174/j.issn.2096-9600.2022.05.04]
 LI Jiqing,LIN Daobin,ZHANG Yongjie.Effects of Sijun Xiaopi Decoction on Gastrointestinal Motility and Gastrointestinal Hormone in Diabetic Gastroparesis Rats[J].Western Journal of Traditional Chinese Medicine,2022,35(12):17.[doi:10.12174/j.issn.2096-9600.2022.05.04]

备注/Memo

备注/Memo:
收稿日期:2017-03-21 *基金项目:国家自然科学基金资助项目(编号81603586);江苏省中医局资助项目(编号LZ13807)。 作者简介:李慧(1981—),女,硕士学位,副主任医师。研究方向:胃肠动力性疾病的中西医结合诊治。 △通讯作者:夏军权(1964—),男,教授,主任医师。研究方向:胃肠动力性疾病的中西医结合诊治。
更新日期/Last Update: 2017-12-15