[1]赵丽,华夏,谭晔.基于基因芯片研究萝卜硫素对大鼠胃癌组织基因表达的影响[J].西部中医药,2020,33(05):23-27.[doi:10.12174/j.issn.1004-6852.2020.05.06]
　ZHAO Li,HUA Xia,TAN Ye.Effects of Sulforaphane on Gene Expression in Gastric Cancer Tissue of Rats Based on Gene Chip[J].Western Journal of Traditional Chinese Medicine,2020,33(05):23-27.[doi:10.12174/j.issn.1004-6852.2020.05.06]
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基于基因芯片研究萝卜硫素对大鼠胃癌组织基因表达的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年05期
页码:
23-27
栏目:
出版日期:
2020-05-15

文章信息/Info

Title:
Effects of Sulforaphane on Gene Expression in Gastric Cancer Tissue of Rats Based on Gene Chip
文章编号:
1004-6852(2020)05-0023-05
作者:
赵丽，华夏，谭晔
恩施土家族苗族自治州中心医院急诊科，湖北 恩施 445000
Author(s):
ZHAO Li, HUA Xia, TAN Ye
Emergency Department, The Central Hospital of Enshi Tujia and Miao Autonomous Perfecture, Enshi 445000, China
关键词:
胃癌; 基因芯片; 胰岛素样生长因子结合蛋白3; 萝卜硫素; 实验研究
Keywords:
gastric cancer;  gene chip;  IGFBP3;  sulforaphane;  experimental study
分类号:
R285.5
DOI:
10.12174/j.issn.1004-6852.2020.05.06
文献标志码:
A
摘要:
目的：研究萝卜硫素对大鼠胃癌组织中相关基因表达的影响。方法：对萝卜硫素组大鼠灌胃50 mg/kg萝卜硫素，胃癌组大鼠腹腔注射同体积生理盐水，采用基因芯片筛选出萝卜硫素诱导表达差异的基因，利用Real-time PCR方法检测胰岛素样生长因子结合蛋白3(insulin-like growth factor binding protein 3，IGFBP3)、神经纤维蛋白1(Neuropilin-1)、雄性性别决定基因相关高迁移率族盒基因(sex-determining regio of Y chromosome related high-mobility-group box 4，SOX4)、N-myc下游调节基因1(NDRG1)4个基因水平；Western Blot方法检测IGFBP3、NDRG1、SOX4和Neuropilin-1蛋白表达水平。结果：经萝卜硫素治疗后大鼠胃癌组织表达上升的基因为159个(差异倍数＞2.0)，表达下降的基因有67个(差异倍数＜0.5)。这些基因主要参与免疫调节、抗氧化、抗炎、介导细胞增殖及凋亡等生物过程。Real-time PCR发现IGFBP3和NDRG1基因表达明显上调，SOX4和Neuropilin-1基因表达明显下调(P＜0.05)，结果与基因芯片一致。Western Blot实验证实萝卜硫素治疗后仅IGFBP3蛋白表达升高(P＜0.05)，其他3种蛋白表达没有显著差异(P＞0.05)。结论：基因芯片与Real-time PCR 技术相结合可找出萝卜硫素处理后的差异表达基因，可为探讨萝卜硫素抑制胃癌的作用机制提供依据。
Abstract:
Objective: To study the effects of sulforaphane on the related gene expressions in gastric cancer tissue of rats. Methods: The rats in sulforaphane group were drenched with 50 mg/kg of sulforaphane, the rats in gastric cancer group accepted intraperitoneal injection of the same volume of saline, gene chips was used to screen the differential expressions genes induced by sulforaphane, Real-time PCR method was adopted to determine the levels of IGFBP3, Neuropilin-1, SOX4 and NDRG1; Western blot to analyze the levels of IGFBP3, NDRG1, SOX4 and Neuropilin-1 protein. Results: After treated by sulforaphane, 159 genes showed up-regulated expression in gastric cancer tissue of the rats (the fold change＞2.0), 67 genes down-regulated expression (the fold change＜0.5). These genes were mainly involved in biological courses such as immune regulation, anti-oxidation effects, anti-inflammation, mediating cellular proliferation and apoptosis. Real-time PCR suggested that genetic expressions of IGFBP3 and NDRG1 up-regulated, while genetic expressions of SOX4 and Neuropilin-1 down-regulated notably(P＜0.05), and the results were identical to gene chip. Western blot suggested that only the expressions of IGFBP3 protein rose apparently after sulforaphane treatment (P＜0.05), no significant difference was found in the expressions of the other three proteins(P＞0.05). Conclusion: Gene chip combined with Real-time PCR technology could find out the differential expressions gene after sulforaphane management, and it could provide the reference for the discussion on the mechanism of sulforaphane inhibiting gastric cancer.

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备注/Memo

备注/Memo:
收稿日期：2018-09-03作者简介：赵丽(1981—)，女，主管护师。研究方向：消化系统疾病的护理。
更新日期/Last Update: 2020-05-15