[1]许洪波,黎彦宏,蔡兴航,等.玄参配方颗粒制备工艺及质量标准研究[J].西部中医药,2021,34(02):35-38.[doi:10.12174/j.issn.2096-9600.2021.02.09]
　XU Hongbo,LI Yanhong,CAI Xinghang,et al.Study on Preparation Technology and Quality Standard of Xuanshen Dispensing Granules[J].Western Journal of Traditional Chinese Medicine,2021,34(02):35-38.[doi:10.12174/j.issn.2096-9600.2021.02.09]
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玄参配方颗粒制备工艺及质量标准研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
34
期数:
2021年02期
页码:
35-38
栏目:
出版日期:
2021-02-15

文章信息/Info

Title:
Study on Preparation Technology and Quality Standard of Xuanshen Dispensing Granules
作者:
许洪波1, 黎彦宏2, 蔡兴航1, 杨康3, 陈素芸1, 冀春1, 刘乃尧1, 唐志书1
1.陕西中医药大学陕西省中药资源产业化协同创新中心，陕西 咸阳 712083
2.西安市第一医院
3.西安市中医医院
Author(s):
XU Hongbo1, LI Yanhong2, CAI Xinghang1, YANG Kang3, CHEN Suyun1, JI Chun1, LIU Naiyao1, TANG Zhishu1
1.Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization of Shaanxi University of Chinese Medicine, Xianyang 712083, China
2.Xi′an No.1 Hospital
3.Xi′an Hospital of Traditional Chinese Medicine
关键词:
玄参; 配方颗粒; 质量控制; 制剂工艺
Keywords:
dispensing granules; quality control; preparation technology
分类号:
R283
DOI:
10.12174/j.issn.2096-9600.2021.02.09
摘要:
目的制备玄参配方颗粒，并进行质量标准研究。 方法使用正交试验设计优化提取工艺，采用湿法制粒制备玄参配方颗粒，建立薄层色谱（thin layer chromatography，TLC）定性鉴别方法，采用紫外-高效液相色谱法（ultraviolet-high-performance liquid chromatography，UV-HPLC）测定特征性成分哈巴苷和哈巴俄苷的含量。 结果优选的提取工艺为：玄参饮片用水煎煮3次，每次30 min，3次煎煮分别加入10、8和8倍量水；建立的TLC方法，色谱斑点清晰，分离度良好；含量测定方法学考察表明，哈巴苷和哈巴俄苷的线性范围分别为：8.91～89.06 μg/mL，R2=1.000；4.81～48.05 μg/mL，R2=0.9999；平均加样回收率分别为：102.47%（RSD=1.88%），97.78%（RSD=2.70%）。 结论优化的玄参提取工艺稳定可行，质量控制方法简单，重复性好，可为玄参配方颗粒的质量控制提供参考。
Abstract:
ObjectiveTo prepare Xuanshen (Scrophulariae Radix) dispensing granules and study quality standard. MethodsThe orthogonal design was used to optimize the extraction process, wet granulation to prepare Xuanshen dispensing granules, to establish TLC qualitative identification method, UV-HPLC to detect the contents of harpagide and harpagoside. ResultsThe optimal extraction process was as follows: the slices of Xuanshen were boiled three times with water for 30 minutes each time, ten, eight and eight times of water were added each time; established TLC method showed that chromatographic spots were clear with good resolution; the study on the methodology of content determination displayed that the linear ranges of harpagide and harpagoside ranged between 8.91 and 89.06 μg/mL, R2=1.000; 4.81 and 48.05 μg/mL,R2=0.9999; average recovery rate were 102.47% (RSD = 1.88%) and 97.78% (RSD = 2.70%) respectively. ConclusionThe optimized extraction process is stable and feasible, the quality control method is simple and has good repeatability, it could provide the reference for quality control of Xuanshen dispensing granules.

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备注/Memo

备注/Memo:
许洪波（1987—），男，博士学位，讲师。研究方向：中药药效物质研究。国家自然科学基金（81603284）；陕西省教育厅自然科学研究项目（18JK0222）。
更新日期/Last Update: 2021-02-15