[1]陈二林,黄艳辉,刘效栓,等.养阴生肌散中龙胆苦苷的HPLC测定[J].西部中医药,2013,26(10):24-26.
 CHEN Erlin,HUANG Yanhui,LIU Xiaoshuan,et al.Determination of Gentiamarin in YangYin ShengJi Powder by HPLC[J].Western Journal of Traditional Chinese Medicine,2013,26(10):24-26.
点击复制

养阴生肌散中龙胆苦苷的HPLC测定
分享到:

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年10期
页码:
24-26
栏目:
方药·质量分析
出版日期:
2013-10-15

文章信息/Info

Title:
Determination of Gentiamarin in YangYin ShengJi Powder by HPLC
文章编号:
1004-6852(2013)10-0024-03
作者:
陈二林1黄艳辉2刘效栓1李喜香1
1 甘肃省中医院,甘肃 兰州 730050; 2 甘肃省肿瘤医院
Author(s):
CHEN Erlin1 HUANG Yanhui2 LIU Xiaoshuan1 LI Xixiang1
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu Province Cancer Hospital
关键词:
高效液相色谱法养阴生肌散龙胆苦苷
Keywords:
HPLC YangYin ShengJi powder gentiamarin
分类号:
R282
文献标志码:
A
摘要:
目的:建立养阴生肌散中龙胆苦苷的含量测定方法。方法:采用色谱柱为Waters Symmetry Shield-C18柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%磷酸溶液(25∶75);流速为1.0 mL/min,柱温为室温,检测波长为265 nm。结果:龙胆苦苷在0.52~2.60 μg范围内呈良好的线性关系,相关系数r=0.999 4,平均回收率为99.06%,RSD为1.85%(n=6)。结论:该测定方法简便快速、准确可靠,可用于该制剂的质量控制。
Abstract:
Objective: To establish the content determination method of gentiamarin in YangYin ShengJi powder. Method: Chromatographic column was Waters Symmetry Shield-C18 column(4.6 mm×250 mm, 5 μm), mobile phase was acentonitrile-0.2% phosphoric acid(25:75); the flow rate was 1.0mL/min, the column temperature was room temperature, detection wavelength was 265nm. Result: Gentiamarin showed a good linear relationship in the range between 0.52 and 2.60 μg, r=0.999 4, average recovery rate was 99.06%, RSD=1.85% (n=6). Conclusion: The determination method, simple, rapid, accurate and reliable, could be used for quality control of the preparation.

相似文献/References:

[1]邢福军,王小萍,乔登嫣,等.养阴生肌散对患者压疮部位微生物的抑菌作用探讨*[J].西部中医药,2012,25(12):65.
 XING Fu-jun,WANG Xiao-ping,QIAO Deng-yan,et al.Bacteriostatic Activity of YangYin ShengJiSan on Pressure Sore of Patients[J].Western Journal of Traditional Chinese Medicine,2012,25(10):65.
[2]张涛,景明△.归芪通便颗粒的质量标准研究*[J].西部中医药,2013,26(03):31.
 ZHANG Tao,JING Ming,Study on Quality Standard of GuiQi TongBian Granules[J].Western Journal of Traditional Chinese Medicine,2013,26(10):31.
[3]梁云,李彩东△,张伟,等.不同产地珠子草药材中有效成分咖啡酸含量的测定*[J].西部中医药,2013,26(05):22.
 LIANG Yun,LI Caidong,ZHANG Wei,et al.Determination of Caffeic Acid in ZhuZiCao from Different Producing Areas[J].Western Journal of Traditional Chinese Medicine,2013,26(10):22.
[4]戴晓雁,王玉洁,魏学兵.升血颗粒质量标准的研究[J].西部中医药,2013,26(09):31.
 DAI Xiaoyan,WANG Yujie,WEI Xuebing.Study on Quality Standard of ShengXue Granules[J].Western Journal of Traditional Chinese Medicine,2013,26(10):31.
[5]李喜香,刘效栓,张小华,等.养阴生肌散急性毒性及全身过敏性实验研究*[J].西部中医药,2014,27(01):29.
 LI Xixiang,LIU Xiaoshuan,ZHANG Xiaohua,et al.Acute Toxicity Testing and Systemic Allergic Experiment of YangYin ShengJi Powder[J].Western Journal of Traditional Chinese Medicine,2014,27(10):29.
[6]戴晓雁,王玉洁,魏学兵.升血颗粒质量标准研究[J].西部中医药,2014,27(01):31.
 DAI Xiaoyan,WANG Yujie,WEI Xuebing.Study on Quality Standard of ShengXue Granules[J].Western Journal of Traditional Chinese Medicine,2014,27(10):31.
[7]吴志成,张泽国,徐福菊,等.当归配方颗粒质量分析研究*[J].西部中医药,2014,27(04):13.
 WU Zhicheng,ZHANG Zeguo,XU Fuju,et al.Study on the Quality of DangGui Granules[J].Western Journal of Traditional Chinese Medicine,2014,27(10):13.
[8]王林凤,魏国玲,康毅.蒲地兰颗粒的质量控制[J].西部中医药,2014,27(04):17.
 WANG Linfeng,WEI Guoling,KANG Yi.Quality Control of PuDiLan Granules[J].Western Journal of Traditional Chinese Medicine,2014,27(10):17.
[9]刘其平,强生毅,周钢.吴紫糊中左旋紫草素的含量测定*[J].西部中医药,2014,27(08):14.
 LIU Qiping,QIANG Shengyi,ZHOU Gang.Content Determination of Shikonin in WuZi Cataplasm[J].Western Journal of Traditional Chinese Medicine,2014,27(10):14.
[10]张承军,王建良,李川国,等.甘肃产党参中党参炔苷含量的动态研究[J].西部中医药,2014,27(09):7.

备注/Memo

备注/Memo:
收稿日期:2013-03-18 作者简介:陈二林(1979—),男,硕士学位,主管药师。研究方向:中药及制剂质量控制研究。
更新日期/Last Update: 2013-10-15