[1]白万富,石松利△,郑倩男,等.不同产地蒙古扁桃叶中槲皮素含量的比较研究[J].西部中医药,2016,29(03):30-32.
　BAI Wanfu,SHI Songli,ZHENG Qiannan,et al.Comparisons of the Contents of Quercetin in Mongolian Almond Leaf from Different Producing Areas[J].Western Journal of Traditional Chinese Medicine,2016,29(03):30-32.
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不同产地蒙古扁桃叶中槲皮素含量的比较研究()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
29
期数:
2016年03期
页码:
30-32
栏目:
出版日期:
2016-03-15

文章信息/Info

Title:
Comparisons of the Contents of Quercetin in Mongolian Almond Leaf from Different Producing Areas
文章编号:
1004-6852(2016)03-0030-03
作者:
白万富，石松利△，郑倩男，吴培赛
内蒙古科技大学包头医学院药学院，内蒙古 包头 014040
Author(s):
BAI Wanfu, SHI Songli△, ZHENG Qiannan, WU Peisai
Pharmacy School of Baotou Medical College of Inner Mongolia University of Science & Technology,Baotou 014040, China
关键词:
蒙古扁桃; 槲皮素; 高效液相色谱法; 含量测定
Keywords:
Mongolian almond;  quercetin;  HPLC;  content determination
分类号:
R917
文献标志码:
A
摘要:
目的：建立蒙古扁桃叶中槲皮素含量测定的方法，对不同产地蒙古扁桃叶中槲皮素的含量进行比较。方法：采用HPLC法,Shim-Pack VP-ODS C18色谱柱(4.6 mm×250 mm，5 μm)；流动相甲醇-0.2%磷酸(50∶50)；柱温30℃；流速1.0 mL/min；检测波长360 nm；进样量20 μL。结果：线性关系良好，r=0.999 9,回归方程为Y=86130X-6471.9，线性范围为2.5～50 μg/mL，平均加样回收率为100.5%，RSD为1.9%(n=6)。不同产地蒙古扁桃叶中槲皮素的含量存在差异，内蒙古固阳地区的含量最高，贺兰山哈拉乌沟地区的含量最低。结论：本实验首次测定了蒙古扁桃叶中槲皮素的含量并对不同出产地进行比较，方法准确、可靠，可作为蒙古扁桃药材质量评价的指标及资源开发的依据。
Abstract:
Objective: To compare the contents of quercetin contained in Mongolian almond leaf [Amygdalus mongolica(Maxim.) Ricker] form different producing areas by establishing content determination method of quercetin. Methods: HPLC method was adopted, Shim-Pack VP-ODS C18 chromatographic column(4.6 mm×250 mm, 5 μm); mobile phase: methanol-0.2% phosphoric acid(50:50); column temperature 30℃; flow rate was 1.0mL/min; detection wavelength was 360 nm; the sample size was 20 μL. Results: Linear relationship was better, r=0.999 9, regression equation was Y=86130X-6471.9, linear range was from 2.5 to 50 μg/mL, average recovery rate was 100.5%, RSD was 1.9% (n=6). The difference was existed in the comparison of the contents of quercetin of Mongolian almond leaf from different producing areas, the contents of quercetin contained in Mongolian almond from Guyang district were the highest, while these contained in the Mongolian almond from Halawugou district of Helan mountains were the lowest. Conclusion: The contents of quercetin in Mongolian almond leaf are initially detected, and different producing areas are compared in the experiment, the method, accurate and reliable, could be taken as the index of quality evaluation of Mongolian almond and the reference of resource development.

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备注/Memo

备注/Memo:
*基金项目：国家自然科学基金项目(编号81102760)；内蒙古自治区自然科学基金项目(编号2015MS0808)。作者简介：白万富(1982—)，男，硕士学位，讲师。研究方向：中、蒙药及其制剂的化学成分及质量标准研究。△通讯作者：石松利(1981—)，女，博士学位，教授。研究方向：中、蒙药成分及资源开发利用研究。
更新日期/Last Update: 2016-03-15