[1]余渊,程杰△.玉米须总皂苷对非酒精性脂肪肝大鼠SREBP1c和ACCα的影响[J].西部中医药,2018,31(09):21-25.
　YU Yuan,CHENG Jie.Effects of Total Saponins Extracted from Stigmata Maydis on SREPB1c and ACCα of the Rats with Nonalcoholic Fatty Liver Disease[J].Western Journal of Traditional Chinese Medicine,2018,31(09):21-25.
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玉米须总皂苷对非酒精性脂肪肝大鼠SREBP1c和ACCα的影响()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年09期
页码:
21-25
栏目:
出版日期:
2018-09-15

文章信息/Info

Title:
Effects of Total Saponins Extracted from Stigmata Maydis on SREPB1c and ACCα of the Rats with Nonalcoholic Fatty Liver Disease
文章编号:
1004-6852(2018)09-0021-05
作者:
余渊1，程杰2△
1 鄂东医疗集团黄石市中心医院，湖北 黄石 435000； 2 黄石市爱康医院
Author(s):
YU Yuan1, CHENG Jie2△
1 Huangshi Central Hospital, EDong Healthcare, Huangshi 435000, China; 2 Huangshi Municipality Aikang Hospital
关键词:
非酒精性脂肪肝; 固醇调节元件结合蛋白1c; 乙酰辅酶羧化酶α; 玉米须总皂苷; 大鼠
Keywords:
nonalcoholic fatty liver disease;  SREBP1c;  ACCα;  total saponins extracted from stigmata maydis;  the rats
分类号:
R285
文献标志码:
A
摘要:
目的：探讨玉米须总皂苷对非酒精性脂肪肝(Nonalcoholic Fatty Liver Disease，NAFLD)大鼠肝脏固醇调节元件结合蛋白1c(Sterol Regulatory Element Binding Protein 1c，SREBP1c)和乙酰辅酶羧化酶α(Acetyl CoA carboxylase α，ACCα)表达的影响。方法：将雄性SD大鼠60只随机分为正常组，模型组和治疗组，每组20只，造模成功后，治疗组给予玉米须总皂苷4 mg/(kg·d)灌胃，正常组和模型组给生理盐水灌胃，于治疗4周和8周后分别处死大鼠各半，HE染色检测肝脏病理学，普通试剂盒检测血脂，酶联免疫吸附实验(ELISA)试剂盒检测肝功能谷草转氨酶(AST)和谷丙转氨酶(ALT)，同时采用RT-PCR以及蛋白免疫印迹法(Western Blot)检测肝脏组织SREBP1c和ACCα的表达。结果：与正常组相比，模型组大鼠肝功能指标AST和ALT，血脂指标甘油三酯(TG)和游离脂肪酸(FFA)升高(P＜0.05)，与模型组相比，玉米须总皂苷治疗4周后，其AST、ALT、TG以及FFA均下降(P＜0.05)；治疗8周后进一步降低(P＜0.05)；与正常组相比，模型组SREBP1c和ACCα表达升高(P＜0.05)，与模型组相比，玉米须总皂苷治疗4周后降低，治疗8周后进一步降低(P＜0.05)。结论：玉米须总皂苷对非酒精性脂肪肝病大鼠肝功能和血脂有改善作用，这可能与其可以降低肝脏脂肪酸α氧化关键酶SREBP1c和ACCα有关。
Abstract:
Objective: To discuss the effects of total saponins extracted from stigmata maydis (Zea mays L.) on the expressions of Acetyl CoA carboxylase α (ACCα) and sterol regulatory element-binding protein 1c (SREBP1c) in the liver of the rats with nonalcoholic fatty liver disease (NAFLD). Methods: Sixty rats were randomized into the normal group, the model group and the treatment group, 20 rats each group. After modeling successfully, the treatment group were drenched with total saponins extracted from stigmata maydis, 4 mg/(kg·d), the normal group and the model group accepted gastric lavage of physiological saline, the rats were sacrificed in four weeks and eight weeks after treating respectively half and half. Liver pathology was detected by HE staining, blood lipid measured by general Kit, AST and ALT by ELISA Kit, simultaneously, the expressions of ACCα and SREBP1c in the liver tissue were measured by RT-PCR method and Western Blot method. Results: Compared with the normal group, AST, ALT, TG and FFA raised in the rats of the model group(P＜0.05), in four weeks after treating by total saponins extracted from stigmata maydis, AST, ALT, TG and FFA lowered compared with the model group(P＜0.05); they lowered further in eight weeks after treating (P＜0.05); compared with the normal group, the expressions of ACCα and SREBP1c in the model group evaluated (P＜0.05), compared with the model group, the expressionsdecreased in four weeks after treating, and decreased further in eight weeks after treating(P＜0.05). Conclusion: Total saponins extracted from stigmata maydis could improve liver function and blood lipid of NAFLD rats, which might be related to that it could decrease the levels of ACCα and SREBP1c in the liver tissue.

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备注/Memo

备注/Memo:
收稿日期：2017-10-04 *基金项目：2015年湖北省科技计划项目(编号2014HS048)。 作者简介：余渊(1981—)，女，硕士学位，主治医师。研究方向：内分泌疾病的中西医结合治疗。 △通讯作者：程杰(1974—)，男，博士学位，副主任医师。研究方向：内分泌疾病的诊治。
更新日期/Last Update: 2018-09-15