[1]刘光华,于艳,王思源,等.HPLC法同时测定甘露消毒丹中3种成分含量[J].西部中医药,2018,31(09):42-45.
 LIU Guanghua,YU Yan,WANG Siyuan,et al.The Contents of Three Ingredients in GanLu XiaoDu Pills Determined by HPLC Method Simultaneously[J].Western Journal of Traditional Chinese Medicine,2018,31(09):42-45.
点击复制

HPLC法同时测定甘露消毒丹中3种成分含量()
分享到:

《西部中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
31
期数:
2018年09期
页码:
42-45
栏目:
方药·质量分析
出版日期:
2018-09-15

文章信息/Info

Title:
The Contents of Three Ingredients in GanLu XiaoDu Pills Determined by HPLC Method Simultaneously
文章编号:
1004-6852(2018)09-0042-04
作者:
刘光华1于艳2王思源2王贵帮3吴振起2刘娟3
1 辽宁中医药大学基础医学院,辽宁 沈阳 110847; 2 辽宁中医药大学附属医院; 3 辽宁中医药大学研究生院
Author(s):
LIU Guanghua1 YU Yan2 WANG Siyuan2 WANG Guibang3 WU Zhenqi2 LIU Juan3
1 School of Basic Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China; 2 The First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine; 3 Graduate School, Liaoning University of Traditional Chinese Medicine
关键词:
甘露消毒丹绿原酸黄芩苷连翘苷含量测定
Keywords:
GanLu XiaoDu pills chlorogenic acid baicalin phillyrin content determination
分类号:
R284.2
文献标志码:
A
摘要:
目的:建立高效液相色谱法对甘露消毒丹中3种成分(绿原酸、黄芩苷、连翘苷)的分析方法。方法:采用Agilent TC-C18(4.6 mm×250 mm,5 μm)色谱柱,以乙腈(A)-0.1%磷酸水(B)为流动相,梯度洗脱,流速为1.0 mL/min,检测波长为327 nm,0~20分钟后测定绿原酸含量;20分钟以后,230 nm测定黄芩苷和连翘苷含量。结果:甘露消毒丹中3种成分分别为绿原酸在0.0791~1.9775 μg(r=0.999 7),黄芩苷在0.3827~9.5675 μg(r=0.999 9),连翘苷在0.0652~1.630 0 μg(r=0.999 9)范围内线性关系良好;平均回收率分别为98.66%、97.49%和98.52%;平均含量分别为2.389、28.362、4.354 mg/g。结论:该方法准确可靠,分离效果理想,重复性好,可用于甘露消毒丹质量控制。
Abstract:
Objective: To establish the determination method for three ingredients (chlorogenic acid, baicalin and phillyrin) in GanLu XiaoDu pills by HPLC. Methods: Agilent TC-C18 (4.6 mm×250 mm, 5 μm) chromatographic column was used, acetonitrile (A)-0.1% phosphoric acid (B) were taken as the mobile phase, gradient elution, flow rate was 1.0 mL/min, detection wavelength was 327nm, the contents of chlorogenic acid were measured in 0 and 20 minutes, the contents of baicalin and phillyrin were detected in 20 minutes when the detection wavelength was 230nm. Results: The three ingredients of GanLu XiaoDu pills were in the good linear relationship when chlorogenic acid, baicalin and phillyrin were in the ranges of 0.0791~1.9775 μg(r=0.999 7), 0.3827~9.5675 μg(r=0.999 9) and 0.065 2~1.630 0 μg(r=0.999 9); average recovery rate were 98.66%, 97.49% and 98.52% respectively, average contents were 2.389 mg/g, 28.362 mg/g and 4.354 mg/g respectively. Conclusion: The method, accurate, reliable and reproducible, could be used for quality control of GanLu XiaoDu pills.

相似文献/References:

[1]任一杰,张 华△,张喜民,等.复方金银花滴丸质量标准的研究[J].西部中医药,2014,27(06):16.
 REN Yijie,ZHANG Hua,ZHANG Ximin,et al.Study on Quality Standard for Compound JinYinHua Dripping Pills[J].Western Journal of Traditional Chinese Medicine,2014,27(09):16.
[2]鱼光强,高晔珩△.强力感冒片指纹图谱的初步研究[J].西部中医药,2015,28(01):23.
[3]魏小果.甘露消毒丹对四氯化碳所致急性肝损伤大鼠的影响[J].西部中医药,2015,28(07):17.
[4]朱成姣,陈飞,姚梅悦,等.金银木不同部位绿原酸的含量测定[J].西部中医药,2016,29(03):35.
 ZHU Chengjiao,CHEN Fei,YAO Meiyue,et al.Content Determination of Chlorogenic Acid Contained in Different Parts of Amur Honeysuckle[J].Western Journal of Traditional Chinese Medicine,2016,29(09):35.
[5]王晓琳,张丽平,展锐,等.肤宁洁搽剂醇提工艺研究[J].西部中医药,2016,29(04):38.
 WANG Xiaolin,ZHANG Liping,et al.Study on Alcohol Extraction Process of FuNingJie Liniment[J].Western Journal of Traditional Chinese Medicine,2016,29(09):38.
[6]姜华,郭敏,徐世红,等.HPLC法测定忍冬藤痛风颗粒中绿原酸的含量[J].西部中医药,2017,30(04):25.
 JIANG Hua,GUO Min,XU Shihong,et al.Determination of Chlorogenic Acid in RenDongTeng Gouty Granules by HPLC[J].Western Journal of Traditional Chinese Medicine,2017,30(09):25.
[7]刘萍,段雅彬,陈红凯,等.昆仑雪菊中原花青素B2、绿原酸含量测定及对小鼠血糖的影响[J].西部中医药,2018,31(09):13.
 LIU Ping,DUAN Yabin,CHEN Hongkai,et al.Content Determination of Procyanidine B2 and Chlorogenic Acid Contained in Kunlun Chrysanthemum and the Influence on the Levels of Blood Glucose of the Mice[J].Western Journal of Traditional Chinese Medicine,2018,31(09):13.
[8]熊刚,邹华,胡承莲,等.绿原酸基于PI3K/Akt信号通路对结肠癌细胞增殖和凋亡的调控作用[J].西部中医药,2020,33(05):71.[doi:10.12174/j.issn.1004-6852.2020.05.20]
 XIONG Gang,ZOU Hua,HU Chenglian,et al.Effects of Chlorogenic Acid on the Proliferation and Apoptosis of Colon Cancer Cells Mediated by P13k/Akt Signaling Pathway[J].Western Journal of Traditional Chinese Medicine,2020,33(09):71.[doi:10.12174/j.issn.1004-6852.2020.05.20]

备注/Memo

备注/Memo:
收稿日期:2018-02-10 *基金项目:国家自然科学基金青年项目(编号81403293)。 作者简介:刘光华(1974—),女,硕士研究生导师,博士学位,副教授。研究方向:中医药防治感染性疾病。
更新日期/Last Update: 2018-09-15