[1]于妍,王硕仁△.参附注射液对肥大心肌细胞能量代谢变化的影响*[J].西部中医药,2013,26(12):11-14.
　YU Yan,WANG Shuoren.Effects of ShenFu Injection on Energy Metabolism of Hypertrophic Myocardial Cells[J].Western Journal of Traditional Chinese Medicine,2013,26(12):11-14.
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参附注射液对肥大心肌细胞能量代谢变化的影响*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年12期
页码:
11-14
栏目:
出版日期:
2013-12-15

文章信息/Info

Title:
Effects of ShenFu Injection on Energy Metabolism of Hypertrophic Myocardial Cells
文章编号:
1004-6852(2013)12-0011-04
作者:
于妍1，王硕仁2△
1 甘肃省中医院心血管科，甘肃 兰州 730050； 2 北京中医药大学东直门医院
Author(s):
YU Yan1, WANG Shuoren2△
1 Cardiovascular Department of Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Dongzhimen Hospital of Beijing University of Chinese Medicine
关键词:
参附注射液; 心肌肥大; 能量代谢
Keywords:
ShenFu injection;  myocardial hypertrophy;  energy metabolism
分类号:
R285.5
文献标志码:
A
摘要:
目的：探讨心肌肥大过程中有关心肌细胞能量代谢的病理改变以及参附注射液对其的药理作用。方法：提取原代心肌细胞与血管紧张素Ⅱ(AngⅡ)共培养，BCA法检测细胞总蛋白含量、倒置显微镜拍摄并测量细胞直径，高效液相色谱法检测心肌细胞内ATP、ADP、AMP含量并计算腺苷酸含量及能荷水平。在此基础上给予参附注射液，观察参附注射液对心肌细胞能量代谢的药理作用。结果：在心肌细胞肥大过程中，心肌细胞ATP、ADP含量、总腺苷酸含量、能荷水平显著降低(P＜0.01)，AMP含量显著增加(P＜0.01)。参附注射液组较同期模型组，心肌细胞总蛋白含量和心肌细胞直径减少(P＜0.05)，心肌细胞ATP、ADP含量著提高(P＜0.05)，AMP含量明显降低(P＜0.05)，心肌细胞总腺苷酸含量明显升高(P＜0.05)，心肌细胞能荷水平显著提高(P＜0.01)。结论：在心肌肥大过程中，存在能量代谢的紊乱。参附注射液在抑制心肌细胞肥大过程中对能量代谢有益。
Abstract:
Objective: To explore pathological changes about energy metabolism during the course of myocardial hypertrophy and pharmacological action of ShenFu injection for myocardial cells. Method: Neonatal myocardial cells were abstracted and cultured with AngⅡ, total protein content was detected with BCA method, cellular diameter measured with inverted microscope, the contents of ATP, ADP and AMP of myocardial cells by HPLC, the content of adenosine acid and energy charge were calculated. Pharmacological effects of ShenFu injection on energy metabolism were observed when ShenFu injection was administered to myocardial cells. Result: During the process of myocardial hypertrophy, the contents of ATP, ADP and total adenosine acid as well as energy charge were decreased significantly(P＜0.01), the contents of AMP were increased remarkably (P＜0.01). The contents of total protein of myocardial cells and cellular diameter were lowered when ShenFu injection group was compared with the model group(P＜0.05), the contents of ATP and ADP were significantly increased(P＜0.05), AMP contents were lowered obviously (P＜0.05), the contents of total adenosine acid were raised remarkably (P＜0.05), the level of energy charge were improved notably (P＜0.01). Conclusion: There is the disorder of energy metabolism in the process of myocardial hypertrophy. ShenFu injection is helpful to energy metabolism in the course of inhibiting myocardial hypertrophy.

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备注/Memo

备注/Memo:
收稿日期：2013-02-12。*基金项目：北京中医药大学自主选题在读研究生资助项目(编号JYB22-XS073)。作者简介：于妍(1983—)，女，博士学位，主治医师。研究方向：心血管疾病的中西医结合诊治。△通讯作者：王硕仁(1947—)，男，博士研究生导师，主任医师。研究方向：心血管疾病的中西医结合诊治。
更新日期/Last Update: 2013-12-15